The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system
[Yin, Hao; Zhang, Si; Long, Lijuan; Tian, Xinpeng; Luo, Xiongming; He, Sha] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; He, Sha] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Yin, Hang] Guiyang Med Coll, Affiliated Hosp, Guiyang 550001, Guizhou, Peoples R China; [Nan, Haihan] Wenzhou Med Coll, Sch Life Sci, Dept Marine Sci, Wenzhou 325035, Zhejiang, Peoples R China; yinhao@scsio.ac.cn
2013
发表期刊JOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
卷号1315页码:80-85
摘要The mangrove plant Pongamia pinnata (Leguminosae) is well known as a plant pesticide. Previous studies have indicated that the flavonoids are responsible of the biological activities of the plant. A new high-speed counter-current chromatography (HSCCC) method for the separation of three flavonoids, karanjin (1), pinnatin (2), and pongaflavone (3), from P. pinnata was developed in the present study. The lower and intermediate phase (LP and IP) of a new three-phase solvent system, n-hexane-acetonitrile-dichloromethane-water, at a volume ratio of 5:5:1:5, were used as the stationary phases, while the upper phase (UP) was used as the mobile phase, and the volume ratio between the stationary phases in the CCC column could be tuned by varying the initial pumped volume ratio of the stationary phases. The CCC columns containing all three phases of the solvent system were considered combination columns. According to the theories of combination column, it is possible to optimize the retention time of the target compounds by varying the volume ratio of the stationary phases in the HSCCC combination columns, as well as the suitable volume ratios of the stationary phases for the separation of the target compounds were predicted from the partition coefficients of the compounds in the three-phase solvent system. Then, three HSCCC separations using the combination columns with initial pumped LP:IP volume ratios of 1:0, 0.9:0.1, and 0.7:0.3 were performed separately based on the prediction. Three target compounds were prepared with high purity when the initial pumped volume ratio of the stationary phases was 0.9:0.1. The baseline separation of compounds 2 and 3 was achieved on the combination column with an initial pumped volume ratio of 0.7:0.3. Furthermore, the three experiments clearly demonstrated that the retentions and resolutions of the target compounds increased with an increasing volume ratio of IP, which is consistent with the prediction for the retention times for the solutes on combination columns. The method proposed here reduces the need for solvent selection compared with the conventional method and may have broad potential applicability in the preparation of natural products. (C) 2013 Elsevier B.V. All rights reserved.
部门归属LMB
关键词High-speed Counter-current Chromatography (Hsccc) Three-phase Solvent System Combination Column Pongamia Pinnata
学科领域Biochemical Research Methods ; Chemistry, Analytical
资助者This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018). ; This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018). ; This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018). ; This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018).
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资助者This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018). ; This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018). ; This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018). ; This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018).
WOS记录号WOS:000326258000011
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条目标识符http://ir.scsio.ac.cn/handle/344004/10964
专题中科院海洋生物资源可持续利用重点实验室
通讯作者yinhao@scsio.ac.cn
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[Yin, Hao,Zhang, Si,Long, Lijuan,et al. The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1315:80-85.
APA [Yin, Hao.,Zhang, Si.,Long, Lijuan.,Tian, Xinpeng.,Luo, Xiongming.,...&yinhao@scsio.ac.cn.(2013).The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system.JOURNAL OF CHROMATOGRAPHY A,1315,80-85.
MLA [Yin, Hao,et al."The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system".JOURNAL OF CHROMATOGRAPHY A 1315(2013):80-85.
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