中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Biochemical Research Methods ; Chemistry, Analytical
题名: The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system
作者: [Yin, Hao ; Zhang, Si ; Long, Lijuan ; Tian, Xinpeng ; Luo, Xiongming ; He, Sha] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Yin, Hao ; Zhang, Si ; Long, Lijuan ; Tian, Xinpeng ; Luo, Xiongming ; He, Sha] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China ; [Yin, Hang] Guiyang Med Coll, Affiliated Hosp, Guiyang 550001, Guizhou, Peoples R China ; [Nan, Haihan] Wenzhou Med Coll, Sch Life Sci, Dept Marine Sci, Wenzhou 325035, Zhejiang, Peoples R China
通讯作者: yinhao@scsio.ac.cn
关键词: High-speed counter-current ; chromatography (HSCCC) ; Three-phase solvent system ; Combination column ; Pongamia pinnata
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013
卷: 1315, 页:80-85
部门归属: LMB
资助者: This work was financially supported by Projects 41176149 and 41230962 supported by the National Natural Science Foundation of China, the Public Science and Technology Ocean Research Funds Projects (grant: 2013418018), the National Basic Research Program of China (973 Program, grant: 2010CB833800), and the Public Science and Technology Ocean Research Funds Projects (grant: 201305018).
摘要: The mangrove plant Pongamia pinnata (Leguminosae) is well known as a plant pesticide. Previous studies have indicated that the flavonoids are responsible of the biological activities of the plant. A new high-speed counter-current chromatography (HSCCC) method for the separation of three flavonoids, karanjin (1), pinnatin (2), and pongaflavone (3), from P. pinnata was developed in the present study. The lower and intermediate phase (LP and IP) of a new three-phase solvent system, n-hexane-acetonitrile-dichloromethane-water, at a volume ratio of 5:5:1:5, were used as the stationary phases, while the upper phase (UP) was used as the mobile phase, and the volume ratio between the stationary phases in the CCC column could be tuned by varying the initial pumped volume ratio of the stationary phases. The CCC columns containing all three phases of the solvent system were considered combination columns. According to the theories of combination column, it is possible to optimize the retention time of the target compounds by varying the volume ratio of the stationary phases in the HSCCC combination columns, as well as the suitable volume ratios of the stationary phases for the separation of the target compounds were predicted from the partition coefficients of the compounds in the three-phase solvent system. Then, three HSCCC separations using the combination columns with initial pumped LP:IP volume ratios of 1:0, 0.9:0.1, and 0.7:0.3 were performed separately based on the prediction. Three target compounds were prepared with high purity when the initial pumped volume ratio of the stationary phases was 0.9:0.1. The baseline separation of compounds 2 and 3 was achieved on the combination column with an initial pumped volume ratio of 0.7:0.3. Furthermore, the three experiments clearly demonstrated that the retentions and resolutions of the target compounds increased with an increasing volume ratio of IP, which is consistent with the prediction for the retention times for the solutes on combination columns. The method proposed here reduces the need for solvent selection compared with the conventional method and may have broad potential applicability in the preparation of natural products. (C) 2013 Elsevier B.V. All rights reserved.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000326258000011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10964
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system.pdf(828KB)----开放获取View Download

Recommended Citation:
[Yin, Hao; Zhang, Si; Long, Lijuan; Tian, Xinpeng; Luo, Xiongming; He, Sha] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Yin, Hao; Zhang, Si; Long, Lijuan; Tian, Xinpeng; Luo, Xiongming; He, Sha] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Guangdong, Peoples R China; [Yin, Hang] Guiyang Med Coll, Affiliated Hosp, Guiyang 550001, Guizhou, Peoples R China; [Nan, Haihan] Wenzhou Med Coll, Sch Life Sci, Dept Marine Sci, Wenzhou 325035, Zhejiang, Peoples R China.The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system,JOURNAL OF CHROMATOGRAPHY A,2013,1315():80-85
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[[Yin, Hao]'s Articles
[Zhang, Si]'s Articles
[Long, Lijuan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[[Yin, Hao]‘s Articles
[Zhang, Si]‘s Articles
[Long, Lijuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace