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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides
作者: [Wei, Jingguang ; Guo, Minglan ; Yan, Yang ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
通讯作者: qinqw@scsio.ac.cn
关键词: Epinephelus coioides ; Singapore grouper iridovirus (SGIV) ; Thioredoxin-related protein of 14 kDa (Ec-TRP14) ; Antioxidant activity
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2013
卷: 35, 期:5, 页:1670-1676
部门归属: LMB
资助者: This work was supported by grants from National High Technology Development Program of China (863) (2012AA092201), National Basic Research Program of China (973 program) (2012CB114402), the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-Q213, KZCX2-YW-BR-08, SQ201203), the National Natural Science Foundation of China (30930070, 31001124, 30725027, 31202022), and the Guangdong Province Natural Science Foundation (S2011040000953, 2012A020603018).
摘要: Thioredoxin (abbreviated as Trx) is an important ubiquitous disulfide reductase, which can protect organisms against various oxidative stresses. In the present study, a thioredoxin-related protein of 14 kDa (named as Ec-TRP14) was identified from the marine fish grouper, Epinephelus coloides by RACE PCR. The full-length cDNA of Ec-TRP14 was comprised of 1066 bp with a 372 bp open reading frame that encodes a putative protein of 123 amino acids. Similar to most TRP145, Ec-TRP14 contained the conserved motif C-P-D-C. Ec-TRP14 mRNA is predominately expressed in liver, brain and muscle. The expression of Ec-TRP14 was up-regulated in the liver of grouper challenged with SGIV. Ec-TRP14 was recombined and expressed in Escherichia call BL21 (DE3), and the rEc-Ec-TRP14 fusion protein was demonstrated to possess the antioxidant activity. The grouper spleen (GS) cells were treated with a high concentration of rEc-TRP14 (8.3 mu g/ml), which significantly enhanced cells viability under damage caused by viral infection. These results together indicated that Ec-TRP14 could function as an important antioxidant in a physiological context, and might be involved in the responses to viral challenge. (C) 2013 Elsevier Ltd. All rights reserved.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000328006700038
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10968
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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[Wei, Jingguang; Guo, Minglan; Yan, Yang; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China.Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides,FISH & SHELLFISH IMMUNOLOGY,2013,35(5):1670-1676
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文件名: Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides.pdf
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