Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress
[Wang, Yanhong; Luo, Peng; Zhang, Lvping; Hu, Chaoqu; Ren, Chunhua; Xia, Jianjun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; cqhu@scsio.ac.cn
2013
发表期刊MOLECULAR BIOLOGY REPORTS
ISSN0301-4851
卷号40期号:11页码:6213-6221
摘要Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) is an intracellular membrane bound enzyme that utilizes the free energy of ATP to transport Ca2+ against a concentration gradient. In the present study, a new SERCA gene (LvSERCA) from white shrimp (Litopenaeus vannamei) was cloned using suppression subtractive hybridization and rapid amplification of cDNA ends. The full-length cDNA of LvSERCA contained an open reading frame of 3,009 bp coding for 1,002 amino acids with a calculated molecular weight of approximately 109.8 kDa. The identity analysis of the amino acid sequence of LvSERCA showed that it is highly conserved with 10 transmembrane alpha-helices, one P-domain, one A-domain and one N-domain. The phylogenetic analysis revealed that LvSERCA is similar to other Arthropoda SERCA proteins. The mRNA levels of LvSERCA under salinity stress (3 and 40 g L-1) were analyzed by reverse transcription PCR and quantitative real-time PCR. The results showed that LvSERCA was expressed in all tissues detected. LvSERCA mRNA levels were significantly higher under hyper-salinity than hypo-salinity. These results highlight that Ga2+-ATPase plays an essential role in adjustment salinity stress, which may be useful for selective breeding of L. vannamei.
部门归属LMB
关键词Cloning Litopenaeus Vannamei Quantitative Real-time Rt-pcr Rapid Amplification Of Cdna Ends Sarco/endoplasmic Reticulum ca2+-atpase
学科领域Biochemistry & Molecular Biology
资助者This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270). ; This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270). ; This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270). ; This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270).
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语种英语
资助者This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270). ; This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270). ; This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270). ; This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270).
WOS记录号WOS:000325948900018
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10971
专题中科院海洋生物资源可持续利用重点实验室
通讯作者cqhu@scsio.ac.cn
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[Wang, Yanhong,Luo, Peng,Zhang, Lvping,et al. Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress[J]. MOLECULAR BIOLOGY REPORTS,2013,40(11):6213-6221.
APA [Wang, Yanhong.,Luo, Peng.,Zhang, Lvping.,Hu, Chaoqu.,Ren, Chunhua.,...&cqhu@scsio.ac.cn.(2013).Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress.MOLECULAR BIOLOGY REPORTS,40(11),6213-6221.
MLA [Wang, Yanhong,et al."Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress".MOLECULAR BIOLOGY REPORTS 40.11(2013):6213-6221.
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