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学科主题: Biochemistry & Molecular Biology
题名: Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress
作者: [Wang, Yanhong ; Luo, Peng ; Zhang, Lvping ; Hu, Chaoqu ; Ren, Chunhua ; Xia, Jianjun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
通讯作者: cqhu@scsio.ac.cn
关键词: Cloning ; Litopenaeus vannamei ; Quantitative real-time RT-PCR ; Rapid amplification of cDNA ends ; Sarco/endoplasmic reticulum Ca2+-ATPase
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2013
卷: 40, 期:11, 页:6213-6221
部门归属: LMB
资助者: This work was supported by a grant from the National High Technology Research & Development Program of China (863 Program) (2012AA10A404-4); grants from the Science & Technology Promoting Project (Grant no, A201101B01 and A201201B02) from Oceanic & Fishery Bureau of Guangdong Province and Guangdong Province & CAS Cooperation Program (2012B091100270).
摘要: Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) is an intracellular membrane bound enzyme that utilizes the free energy of ATP to transport Ca2+ against a concentration gradient. In the present study, a new SERCA gene (LvSERCA) from white shrimp (Litopenaeus vannamei) was cloned using suppression subtractive hybridization and rapid amplification of cDNA ends. The full-length cDNA of LvSERCA contained an open reading frame of 3,009 bp coding for 1,002 amino acids with a calculated molecular weight of approximately 109.8 kDa. The identity analysis of the amino acid sequence of LvSERCA showed that it is highly conserved with 10 transmembrane alpha-helices, one P-domain, one A-domain and one N-domain. The phylogenetic analysis revealed that LvSERCA is similar to other Arthropoda SERCA proteins. The mRNA levels of LvSERCA under salinity stress (3 and 40 g L-1) were analyzed by reverse transcription PCR and quantitative real-time PCR. The results showed that LvSERCA was expressed in all tissues detected. LvSERCA mRNA levels were significantly higher under hyper-salinity than hypo-salinity. These results highlight that Ga2+-ATPase plays an essential role in adjustment salinity stress, which may be useful for selective breeding of L. vannamei.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000325948900018
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10971
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
[Wang, Yanhong; Luo, Peng; Zhang, Lvping; Hu, Chaoqu; Ren, Chunhua; Xia, Jianjun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.Cloning of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) gene from white shrimp, Litopenaeus vannamei and its expression level analysis under salinity stress,MOLECULAR BIOLOGY REPORTS,2013,40(11):6213-6221
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