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Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats
[Liu, Guodong; Dong, Jun; Huang, Qiang; Lan, Qing] Soochow Univ, Affiliated Hosp 2, Dept Neurosurg, Suzhou 215004, Peoples R China; [Mao, Jinning] Soochow Univ, Affiliated Hosp 2, Cardiovasc Dept, Suzhou 215004, Peoples R China; [Jiang, Zirong] Changyi Peoples Hosp, Changyi, Peoples R China; [Sun, Tao] Xian Jiaotong Liverpool Univ, Dept Biol Sci, Suzhou, Peoples R China; [Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Jiang, Zhen; Zhang, Caiyuan] Soochow Univ, Affiliated Hosp 2, Dept Radiol, Suzhou 215004, Peoples R China; lanqingsci@163.com
2013
Source PublicationCANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
ISSN1084-9785
Volume28Issue:9Pages:691-696
AbstractDoxorubicin (Dox) is widely used for the treatment of solid tumors but its clinical utility on glioma is limited. In this study, we developed a novel nano-scale drug delivery system employing biodegradable nanoparticle (NP) as carriers to load Dox. Transferrin (Tf) was conjugated to the surface of NP to specifically target the NP to glioma. Tf-NP-Dox was prepared via emulsification-solvent evaporation method, and characterized for the size, Drug loading capacity (DLC), entrapment efficiency, and Tf number on the surface. The antitumor efficiency in vitro was evaluated via CCK-8 assay. The transmembrane transportation was evaluated via HPLC assay. The antitumor efficiency in vivo was assessed in C6 glioma intracranial implant rat model. The average diameter of Tf-NP-Dox was 100nm with approximate to 32 Tf molecules on the surface. DLC was 4.4%. CCK-8 assay demonstrated much stronger cytotoxicity of Tf-NP-Dox to C6 glioma cells compared to NP-Dox or Dox. HPLC assay showed that Tf-NP-Dox transported Dox into C6 cells with high efficiency. In vivo, Tf-NP-Dox could transport Dox into tumors compare to contralateral part, with tumor inhibitory ratio and survival higher than NP-Dox or Dox. Taken together, our results suggest that Tf-NP-Dox exhibits better therapeutic effects against glioma both in vitro and in vivo, and is a potential nano-scale drug delivery system for glioma chemotherapy.
DepartmentLMB
KeywordChemotherapy Doxorubicin Glioma Nanoparticle Transferrin
Subject AreaOncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
Funding OrganizationThis work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554).
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Language英语
Funding OrganizationThis work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554).
WOS IDWOS:000325267700007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10972
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorlanqingsci@163.com
Recommended Citation
GB/T 7714
[Liu, Guodong,Dong, Jun,Huang, Qiang,et al. Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2013,28(9):691-696.
APA [Liu, Guodong.,Dong, Jun.,Huang, Qiang.,Lan, Qing] Soochow Univ, Affiliated Hosp 2, Dept Neurosurg, Suzhou 215004, Peoples R China.,[Mao, Jinning] Soochow Univ, Affiliated Hosp 2, Cardiovasc Dept, Suzhou 215004, Peoples R China.,...&lanqingsci@163.com.(2013).Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,28(9),691-696.
MLA [Liu, Guodong,et al."Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 28.9(2013):691-696.
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