Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats
[Liu, Guodong; Dong, Jun; Huang, Qiang; Lan, Qing] Soochow Univ, Affiliated Hosp 2, Dept Neurosurg, Suzhou 215004, Peoples R China; [Mao, Jinning] Soochow Univ, Affiliated Hosp 2, Cardiovasc Dept, Suzhou 215004, Peoples R China; [Jiang, Zirong] Changyi Peoples Hosp, Changyi, Peoples R China; [Sun, Tao] Xian Jiaotong Liverpool Univ, Dept Biol Sci, Suzhou, Peoples R China; [Hu, Yunfeng] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; [Jiang, Zhen; Zhang, Caiyuan] Soochow Univ, Affiliated Hosp 2, Dept Radiol, Suzhou 215004, Peoples R China; lanqingsci@163.com
2013
发表期刊CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
ISSN1084-9785
卷号28期号:9页码:691-696
摘要Doxorubicin (Dox) is widely used for the treatment of solid tumors but its clinical utility on glioma is limited. In this study, we developed a novel nano-scale drug delivery system employing biodegradable nanoparticle (NP) as carriers to load Dox. Transferrin (Tf) was conjugated to the surface of NP to specifically target the NP to glioma. Tf-NP-Dox was prepared via emulsification-solvent evaporation method, and characterized for the size, Drug loading capacity (DLC), entrapment efficiency, and Tf number on the surface. The antitumor efficiency in vitro was evaluated via CCK-8 assay. The transmembrane transportation was evaluated via HPLC assay. The antitumor efficiency in vivo was assessed in C6 glioma intracranial implant rat model. The average diameter of Tf-NP-Dox was 100nm with approximate to 32 Tf molecules on the surface. DLC was 4.4%. CCK-8 assay demonstrated much stronger cytotoxicity of Tf-NP-Dox to C6 glioma cells compared to NP-Dox or Dox. HPLC assay showed that Tf-NP-Dox transported Dox into C6 cells with high efficiency. In vivo, Tf-NP-Dox could transport Dox into tumors compare to contralateral part, with tumor inhibitory ratio and survival higher than NP-Dox or Dox. Taken together, our results suggest that Tf-NP-Dox exhibits better therapeutic effects against glioma both in vitro and in vivo, and is a potential nano-scale drug delivery system for glioma chemotherapy.
部门归属LMB
关键词Chemotherapy Doxorubicin Glioma Nanoparticle Transferrin
学科领域Oncology ; Medicine, Research & Experimental ; Pharmacology & Pharmacy ; Radiology, Nuclear Medicine & Medical Imaging
资助者This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554).
URL查看原文
语种英语
资助者This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554). ; This work was supported by the University Graduate Students' Scientific Research Innovative Program of Jiangsu Province, China (CX10B-0502) and the National Science Foundation of People's Republic of China (81272799, 81271554).
WOS记录号WOS:000325267700007
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10972
专题中科院海洋生物资源可持续利用重点实验室
通讯作者lanqingsci@163.com
推荐引用方式
GB/T 7714
[Liu, Guodong,Dong, Jun,Huang, Qiang,et al. Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats[J]. CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,2013,28(9):691-696.
APA [Liu, Guodong.,Dong, Jun.,Huang, Qiang.,Lan, Qing] Soochow Univ, Affiliated Hosp 2, Dept Neurosurg, Suzhou 215004, Peoples R China.,[Mao, Jinning] Soochow Univ, Affiliated Hosp 2, Cardiovasc Dept, Suzhou 215004, Peoples R China.,...&lanqingsci@163.com.(2013).Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats.CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS,28(9),691-696.
MLA [Liu, Guodong,et al."Transferrin-Modified Doxorubicin-Loaded Biodegradable Nanoparticles Exhibit Enhanced Efficacy in Treating Brain Glioma-Bearing Rats".CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS 28.9(2013):691-696.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Transferrin-Modified(339KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。