中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Biology
题名: Sensitivity of ventilation and brain metabolism to ammonia exposure in rainbow trout, Oncorhynchus mykiss
作者: [Zhang, Li ; Nawata, C. Michele ; Wood, Chris M.] McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada ; [Zhang, Li] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Nawata, C. Michele] Univ Arizona, Dept Physiol, Tucson, AZ 85724 USA
通讯作者: zhangli@scsio.ac.cn
关键词: Rh protein ; ammonia ; brain ; fish ; glutamine synthetase ; ventilation
刊名: JOURNAL OF EXPERIMENTAL BIOLOGY
发表日期: 2013
卷: 216, 期:21, 页:4025-4037
部门归属: LMB
资助者: This work was supported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant and a Canada Foundation for Innovation (CFI) award to C. M. W., who is also supported by the Canada Research Chairs Program.
摘要: Ammonia has been documented as a respiratory gas that stimulates ventilation, and is sensed by peripheral neuroepithelial cells (NECs) in the gills in ammoniotelic rainbow trout. However, the hyperventilatory response is abolished in trout chronically exposed (1+ months) to high environmental ammonia [HEA; 250 mu mol l(-1) (NH4)(2)SO4]. This study investigates whether the brain is involved in the acute sensitivity of ventilation to ammonia, and whether changes in brain metabolism are related to the loss of hyperventilatory responses in trout chronically exposed to HEA ('HEA trout'). Hyperventilation (via increased ventilatory amplitude rather than rate) and increased total ammonia concentration ([T-Amm]) in brain tissue were induced in parallel by acute HEA exposure in control trout in a concentration-series experiment [500, 750 and 1000 mu mol l(-1) (NH4)(2)SO4], but these inductions were abolished in HEA trout. Ventilation was correlated more closely to [T-Amm] in brain rather than to [T-Amm] in plasma or cerebrospinal fluid. The close correlation of hyperventilation and increased brain [T-Amm] also occurred in control trout acutely exposed to HEA in a time-series analysis [500 mu mol l(-1) (NH4)(2)SO4; 15, 30, 45 and 60 min], as well as in a methionine sulfoxamine (MSOX) pre-injection experiment [to inhibit glutamine synthetase (GSase)]. These correlations consistently suggest that brain [T-Amm] is involved in the hyperventilatory responses to ammonia in trout. The MSOX treatments, together with measurements of GSase activity, T-Amm, glutamine and glutamate concentrations in brain tissue, were conducted in both the control and HEA trout. These experiments revealed that GSase plays an important role in transferring ammonia to glutamate to make glutamine in trout brain, thereby attenuating the elevation of brain [T-Amm] following HEA exposure, and that glutamate concentration is reduced in HEA trout. The mRNAs for the ammonia channel proteins Rhbg, Rhcg1 and Rhcg2 were expressed in trout brain, and the expression of Rhbg and Rhcg2 increased in HEA trout, potentially as a mechanism to facilitate the efflux of ammonia. In summary, the brain appears to be involved in the sensitivity of ventilation to ammonia, and brain ammonia levels are regulated metabolically in trout.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000325806300018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10975
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Sensitivity of ventilation and brain metabolism to ammonia exposure in rainbow trout, Oncorhynchus mykiss.pdf(577KB)----开放获取View Download

Recommended Citation:
[Zhang, Li; Nawata, C. Michele; Wood, Chris M.] McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada; [Zhang, Li] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Nawata, C. Michele] Univ Arizona, Dept Physiol, Tucson, AZ 85724 USA.Sensitivity of ventilation and brain metabolism to ammonia exposure in rainbow trout, Oncorhynchus mykiss,JOURNAL OF EXPERIMENTAL BIOLOGY,2013,216(21):4025-4037
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[[Zhang, Li]'s Articles
[Nawata, C. Michele]'s Articles
[Wood, Chris M.] McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[[Zhang, Li]‘s Articles
[Nawata, C. Michele]‘s Articles
[Wood, Chris M.] McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Sensitivity of ventilation and brain metabolism to ammonia exposure in rainbow trout, Oncorhynchus mykiss.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace