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学科主题: Biotechnology & Applied Microbiology ; Virology
题名: Medaka haploid embryonic stem cells are susceptible to Singapore grouper iridovirus as well as to other viruses of aquaculture fish species
作者: [Yuan, Yongming ; Zhang, Lei ; Zhu, Yi ; Hong, Yunhan] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore ; [Huang, Xiaohong ; Huang, Youhua ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China
通讯作者: qinqw@scsio.ac.cn ; dbshyh@nus.edu.sg
刊名: JOURNAL OF GENERAL VIROLOGY
发表日期: 2013
卷: 94, 页:2352-2359
部门归属: LMB
资助者: We thank J. Deng for fish breeding and C. M. Foong for laboratory management. This work was supported by the National Research Foundation Singapore (NRF-CRP7-2010-03) and the National Basic Research Program of China (973 Program: 2012CB114402).
摘要: Viral infection is a challenge in high-density aquaculture, as it leads to various diseases and causes massive or even complete loss. The identification and disruption of host factors that viruses utilize for infection offer a novel approach to generate viral-resistant seed stocks for cost-efficient and sustainable aquaculture. Genetic screening in haploid cell cultures represents an ideal tool for host factor identification. We have recently generated haploid embryonic stem (ES) cells in the laboratory fish medaka. Here, we report that HX1, one of the three established medaka haploid ES cell lines, was susceptible to the viruses tested and is thus suitable for genetic screening to identify host factors. HX1 cells displayed a cytopathic effect and massive death upon inoculation with three highly infectious and notifiable fish viruses, namely Singapore grouper iridovirus (SGIV), spring viremia of carp virus (SVCV) and red-spotted grouper nervous necrosis virus (RGNNV). Reverse transcription-PCR and Western blot analyses revealed the expression of virus genes. SGIV infection in HX1 cells elicited a host immune response and apoptosis. Viral replication kinetics were determined from a virus growth curve, and electron microscopy revealed propagation, assembly and release of infectious SGIV particles in HX1 cells. Our results demonstrate that medaka haploid ES cells are susceptible to SGIV, as well as to SVCV and RGNNV, offering a unique opportunity for the identification of host factors by genetic screening.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000326215000021
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/10981
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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[Yuan, Yongming; Zhang, Lei; Zhu, Yi; Hong, Yunhan] Natl Univ Singapore, Dept Biol Sci, Singapore 117548, Singapore; [Huang, Xiaohong; Huang, Youhua; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China.Medaka haploid embryonic stem cells are susceptible to Singapore grouper iridovirus as well as to other viruses of aquaculture fish species,JOURNAL OF GENERAL VIROLOGY,2013,94():2352-2359
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