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Characteristic and Functional Analysis of Toll-like Receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, Reveals Ancient Origin of TLR-Mediated Innate Immunity
[Zhang, Yang; Yu, Feng; Xiang, Zhiming; Li, Jun; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China; [He, Xiaocui] Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Inst Immunol, Greifswald, Germany; [Thorpe, Karen L.] Univ Portsmouth, Inst Marine Sci, Portsmouth, Hants, England; carlzyu@scsio.ac.cn
2013
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:10Pages:-e76464
AbstractThe evolution of TLR-mediated innate immunity is a fundamental question in immunology. Here, we report the characterization and functional analysis of four TLR members in the lophotrochozoans Crassostrea gigas (CgTLRs). All CgTLRs bear a conserved domain organization and have a close relationship with TLRs in ancient non-vertebrate chordates. In HEK293 cells, every CgTLR could constitutively activate NF-kappa B responsive reporter, but none of the PAMPs tested could stimulate CgTLR-activated NF-kappa B induction. Subcellular localization showed that CgTLR members have similar and dual distribution on late endosomes and plasma membranes. Moreover, CgTLRs and CgMyD88 mRNA show a consistent response to multiple PAMP challenges in oyster hemocytes. As CgTLR-mediated NF-kappa B activation is dependent on CgMyD88, we designed a blocking peptide for CgTLR signaling that would inhibit CgTLR-CgMyD88 dependent NF-kappa B activation. This was used to demonstrate that a Vibrio parahaemolyticus infection-induced enhancement of degranulation and increase of cytokines TNF mRNA in hemocytes, could be inhibited by blocking CgTLR signaling. In summary, our study characterized the primitive TLRs in the lophotrocozoan C. gigas and demonstrated a fundamental role of TLR signaling in infection-induced hemocyte activation. This provides further evidence for an ancient origin of TLR-mediated innate immunity.
DepartmentLMB
Subject AreaMultidisciplinary Sciences
Funding OrganizationThis work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Language英语
Funding OrganizationThis work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by the National Basic Research Program of China (No. 2010CB126404), the National Natural Science Foundation of China (No. 41176150 and 31001130) and Joint Funds of NSFC-Guangdong of China (U1201215). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS IDWOS:000319646100189
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10983
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcarlzyu@scsio.ac.cn
Recommended Citation
GB/T 7714
[Zhang, Yang,Yu, Feng,Xiang, Zhiming,et al. Characteristic and Functional Analysis of Toll-like Receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, Reveals Ancient Origin of TLR-Mediated Innate Immunity[J]. PLOS ONE,2013,8(10):-e76464.
APA [Zhang, Yang.,Yu, Feng.,Xiang, Zhiming.,Li, Jun.,Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou, Guangdong, Peoples R China.,...&carlzyu@scsio.ac.cn.(2013).Characteristic and Functional Analysis of Toll-like Receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, Reveals Ancient Origin of TLR-Mediated Innate Immunity.PLOS ONE,8(10),-e76464.
MLA [Zhang, Yang,et al."Characteristic and Functional Analysis of Toll-like Receptors (TLRs) in the lophotrocozoan, Crassostrea gigas, Reveals Ancient Origin of TLR-Mediated Innate Immunity".PLOS ONE 8.10(2013):-e76464.
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