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First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea
[Xie Zhenyu; Ke Shaowen; Zhu Zhixiong; Wang Shifeng; Zhou Yongcan] Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou, Peoples R China; Zhou Yongcan] Hainan Univ, Hainan Prov Key Lab Trop Hydrobiol & Biotechnol, Haikou, Peoples R China; [Hu Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Marine Bioresources Sustainable Utili, Guangzhou, Guangdong, Peoples R China; zychnu@163.com
2013
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:9Pages:-e75425
AbstractBackground: White syndrome, a term for scleractinian coral disease with progressive tissue loss, is known to cause depressed growth and increased morality of coral reefs in the major oceans around the world, and the occurrence of this disease has been frequently reported in the past few decades. Investigations during April to September in both 2010 and 2011 identified widespread Porites andrewsi White syndrome (PAWS) in Xisha Archipelago, South China Sea. However, the causes and etiology of PAWS have been unknown. Methodology/Principal Findings: A transmission experiment was performed on P. andrewsi in the Qilianyu Subgroup (QLY). The results showed that there was a significant (P <= 0.05) difference between test and control groups after 28 days if the invalid replicates were excluded. Rates of tissue loss ranged from 0.90-10.76 cm(2) d(-1) with a mean of 5.40 +/- 3.34 cm(2) d(-1) (mean +/- SD). Bacterial strains were isolated from the PAWS corals at the disease outbreak sites in QLY of the Xisha Archipelago, South China Sea, and included in laboratory-based infection trials to satisfy Koch's postulates for establishing causality. Following exposure to bacterial concentrations of 10(5) cells mL(-1), the infected colonies exhibited similar signs to those observed in the field. Using phylogenetic 16S rRNA gene analysis, classical phenotypic trait comparison, Biolog automatic identification system, MALDI-TOF mass spectrometry and MALDI Biotyper method, two pathogenic strains were identified as Vibrio alginolyticus. Conclusion/Significance: This is the first report of V. alginolyticus as a pathogenic agent of PAWS in the South China Sea. Our results point out an urgent need to develop sensitive detection methods for V. alginolyticus virulence strains and robust diagnostics for coral disease caused by this and Vibrio pathogenic bacterium in the South China Sea.
DepartmentLMB
Subject AreaMultidisciplinary Sciences
Funding OrganizationThis work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Language英语
Funding OrganizationThis work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS IDWOS:000325025200042
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/10984
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorzychnu@163.com
Recommended Citation
GB/T 7714
[Xie Zhenyu,Ke Shaowen,Zhu Zhixiong,et al. First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea[J]. PLOS ONE,2013,8(9):-e75425.
APA [Xie Zhenyu.,Ke Shaowen.,Zhu Zhixiong.,Wang Shifeng.,Zhou Yongcan] Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou, Peoples R China.,...&zychnu@163.com.(2013).First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea.PLOS ONE,8(9),-e75425.
MLA [Xie Zhenyu,et al."First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea".PLOS ONE 8.9(2013):-e75425.
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