First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea
[Xie Zhenyu; Ke Shaowen; Zhu Zhixiong; Wang Shifeng; Zhou Yongcan] Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou, Peoples R China; Zhou Yongcan] Hainan Univ, Hainan Prov Key Lab Trop Hydrobiol & Biotechnol, Haikou, Peoples R China; [Hu Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Marine Bioresources Sustainable Utili, Guangzhou, Guangdong, Peoples R China; zychnu@163.com
2013
发表期刊PLOS ONE
ISSN1932-6203
卷号8期号:9页码:-e75425
摘要Background: White syndrome, a term for scleractinian coral disease with progressive tissue loss, is known to cause depressed growth and increased morality of coral reefs in the major oceans around the world, and the occurrence of this disease has been frequently reported in the past few decades. Investigations during April to September in both 2010 and 2011 identified widespread Porites andrewsi White syndrome (PAWS) in Xisha Archipelago, South China Sea. However, the causes and etiology of PAWS have been unknown. Methodology/Principal Findings: A transmission experiment was performed on P. andrewsi in the Qilianyu Subgroup (QLY). The results showed that there was a significant (P <= 0.05) difference between test and control groups after 28 days if the invalid replicates were excluded. Rates of tissue loss ranged from 0.90-10.76 cm(2) d(-1) with a mean of 5.40 +/- 3.34 cm(2) d(-1) (mean +/- SD). Bacterial strains were isolated from the PAWS corals at the disease outbreak sites in QLY of the Xisha Archipelago, South China Sea, and included in laboratory-based infection trials to satisfy Koch's postulates for establishing causality. Following exposure to bacterial concentrations of 10(5) cells mL(-1), the infected colonies exhibited similar signs to those observed in the field. Using phylogenetic 16S rRNA gene analysis, classical phenotypic trait comparison, Biolog automatic identification system, MALDI-TOF mass spectrometry and MALDI Biotyper method, two pathogenic strains were identified as Vibrio alginolyticus. Conclusion/Significance: This is the first report of V. alginolyticus as a pathogenic agent of PAWS in the South China Sea. Our results point out an urgent need to develop sensitive detection methods for V. alginolyticus virulence strains and robust diagnostics for coral disease caused by this and Vibrio pathogenic bacterium in the South China Sea.
部门归属LMB
学科领域Multidisciplinary Sciences
资助者This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
URL查看原文
语种英语
资助者This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This work was supported by grants from the National Science and Technology Support Program (no. 2009BAB44B02); the National Natural Science Foundations of China (no. 41066003, no. 31060360 and no. 30760190); and the Project of Fok Ying Tong Education Foundation (no. 121030). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS记录号WOS:000325025200042
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/10984
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zychnu@163.com
推荐引用方式
GB/T 7714
[Xie Zhenyu,Ke Shaowen,Zhu Zhixiong,et al. First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea[J]. PLOS ONE,2013,8(9):-e75425.
APA [Xie Zhenyu.,Ke Shaowen.,Zhu Zhixiong.,Wang Shifeng.,Zhou Yongcan] Hainan Univ, Hainan Key Lab Sustainable Utilizat Trop Bioresou, Haikou, Peoples R China.,...&zychnu@163.com.(2013).First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea.PLOS ONE,8(9),-e75425.
MLA [Xie Zhenyu,et al."First Characterization of Bacterial Pathogen, Vibrio alginolyticus, for Porites andrewsi White Syndrome in the South China Sea".PLOS ONE 8.9(2013):-e75425.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
First Characterizati(1385KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。