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Development of 25 Novel Microsatellite Loci and Genetic Variation Analysis in Breeding Populations of the Pearl Oyster, Pinctada fucata
[Wu, Shanzeng; Guan, Yunyan; Huang, Xiande; He, Maoxian] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Wu, Shanzeng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2013
Source PublicationJOURNAL OF THE WORLD AQUACULTURE SOCIETY
ISSN0893-8849
Volume44Issue:4Pages:600-609
AbstractIn this study, we screened 103,050 gene sequences of Pinctada fucata and identified 1450 simple sequence repeats (SSRs) distributing in 1355 sequences, in which 97 have sufficient flanking sequences for primer design, and 25 novel microsatellite loci were stable and polymorphic with Na which varied from 2 to 6. Six microsatellite loci were found in the open reading frame of their corresponding sequences. With the 25 SSR loci, five breeding populations and a wild population, a total of 240 individuals from wild population (W), three consecutive selected populations (G1, G2, and G3), and two backcross populations (Do, Dr) (40 individuals per population) were assayed. In these populations, we detected 108 alleles of 111 bp to 362 bp. Each population had mean Ho of 0.5718-0.7366 and mean He of 0.5830-0.6954. Except the Fst = 0.0697 (W vs. G3), pairwise Fst values ranged from 0.0131 to 0.0473, the genetic identity coefficients varied from 0.8688 to 0.9663, and genetic distance ranged from 0.0343 to 0.1307, all of these suggested that the breeding populations had genetic differentiation but at a low level generally. Besides, all the genetic index values, backcross populations (Do, Dr) were higher than corresponding G3 values, indicating that backcross could make the offspring incline to the recurrent parents.
DepartmentLMB
Subject AreaFisheries
Funding OrganizationThe pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05).
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Language英语
Funding OrganizationThe pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was financially supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and Marine fishery science and technology promotion program of Guangdong Province, China (A201201A05).
WOS IDWOS:000322597200014
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11004
Collection中科院海洋生物资源可持续利用重点实验室
Recommended Citation
GB/T 7714
[Wu, Shanzeng,Guan, Yunyan,Huang, Xiande,et al. Development of 25 Novel Microsatellite Loci and Genetic Variation Analysis in Breeding Populations of the Pearl Oyster, Pinctada fucata[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2013,44(4):600-609.
APA [Wu, Shanzeng,Guan, Yunyan,Huang, Xiande,He, Maoxian] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China,&[Wu, Shanzeng] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.(2013).Development of 25 Novel Microsatellite Loci and Genetic Variation Analysis in Breeding Populations of the Pearl Oyster, Pinctada fucata.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,44(4),600-609.
MLA [Wu, Shanzeng,et al."Development of 25 Novel Microsatellite Loci and Genetic Variation Analysis in Breeding Populations of the Pearl Oyster, Pinctada fucata".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 44.4(2013):600-609.
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