中国科学院南海海洋研究所机构知识库
Advanced  
SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室  > 期刊论文
学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas
作者: [Li, Jun ; Zhang, Yang ; Xiang, Zhiming ; Xiao, Shu ; Yu, Feng ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
通讯作者: carlzyu@scsio.ac.cnv
关键词: Crassostrea gigas ; HMGB1 ; NF-kappa B ; Sub-cellular localization ; Pro-inflammatory cytokine
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2013
卷: 35, 期:1, 页:63-70
部门归属: LMB
资助者: This work was supported by a National Basic Research Program of China (No. 2010CB126404) and National Science Foundation of China (No. 31001130 and 41176150). We also thank Prof. Elizabeth De Stasio for critical editing of the manuscript.
摘要: High-mobility group box 1 (HMGB1), a highly conserved DNA-binding protein, is involved in nucleosome formation and transcriptional regulation, and can also act as an extracellular cytokine to trigger inflammation and immune responses. In this study, we identified a HMGB1 gene (hereafter designated as CgHMGB1) in the Pacific oyster Crassostrea gigas. The full-length CgHMGB1 cDNA is 833 bp including 5' and 3'-untranslated regions (UTRs) of 145 and 79 bp, respectively, and an open reading frame (ORF) of 609 bp. The gene encodes a 202 amino acid polypeptide with an estimated molecular mass of 23.3 kDa. Sequence alignment shows that CgHMGB1 contains two basic HMG boxes and a highly acidic C-terminal domain. Recombinant CgHMGB1 proteins can enhance the mRNA level of various inflammatory cytokines in vivo. Typically, CgHMGB1 is localized in the nucleus, though lipopolysaccharide can induce its release to cytoplasm. Moreover, luciferase reporter assays reveal that CgHMGB1 cannot stimulate Nuclear Factor-kappa B reporter activity alone, but it can enhance Rel-dependent NF-kappa B activation in a dose-dependent manner. CgHMGB1 is highly expressed in hemocytes and its transcripts are significantly more abundant following bacterial challenge. Our results suggest that CgHMGB1 plays an essential role in innate defense by enhancing Rel-activated NF-kappa B activity and inducing the expression of inflammatory cytokines. (c) 2013 Elsevier Ltd. All rights reserved.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000320749700008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11015
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas.pdf(1213KB)----开放获取View Download

Recommended Citation:
[Li, Jun; Zhang, Yang; Xiang, Zhiming; Xiao, Shu; Yu, Feng; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas,FISH & SHELLFISH IMMUNOLOGY,2013,35(1):63-70
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[[Li, Jun]'s Articles
[Zhang, Yang]'s Articles
[Xiang, Zhiming]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[[Li, Jun]‘s Articles
[Zhang, Yang]‘s Articles
[Xiang, Zhiming]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: High mobility group box 1 can enhance NF-kappa B activation and act as a pro-inflammatory molecule in the Pacific oyster, Crassostrea gigas.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院南海海洋研究所 - Feedback
Powered by CSpace