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学科主题: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Cell Biology
题名: Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems
作者: [Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS LMB, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
通讯作者: cqhu@scsio.ac.cn
关键词: Vibrio alginolyticus ; Challenge ; Pathogenicity ; Virulence associated genes ; Detect ; Relationship
刊名: MOLECULAR AND CELLULAR PROBES
发表日期: 2013
卷: 27, 期:41702, 页:164-168
部门归属: LMB
资助者: This study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02).
摘要: Vibrio alginolyticus has been confirmed as an important pathogen for aquatic animals. However, the pathogenic mechanism of V. alginolyticus is not completely understood. A total of 31 isolates of V. alginolyticus from sea water, fish and shrimp on the mariculture systems were fingerprinted by pulsed-field gel electrophoresis. The pathogenicity of these isolates was tested by challenge and the 21 genes associated with the virulence of Vibrio cholerae or Vibrio parahaemolyticus were examined in V. alginolyticus using PCR. The results showed that the 31 V. alginolyticus isolates belonged to 26 PFGE genotypes and the isolates from different source had different genotypes. Nine of the 31 isolates were confirmed as pathogenic strains by challenge. Moreover, 12 vibrio virulence genes were detected in this study. Of the detected genes, VCtoxR, VCtoxS, hlyA, VPtoxR and tlh were found in both pathogenic and non-pathogenic isolates. However, the other 7 virulence genes, ctxB, zot, tagA, stn, sto, tdh and trh, were only present in pathogenic isolates. Analysis of the relationship between virulence associated genes and pathogenicity of V. alginolyticus provides a possible explanation that the pathogenic mechanism of V. alginolyticus might be similar to that of V. parahaemolyticus instead of V. cholerae. (c) 2013 Elsevier Ltd. All rights reserved.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000318961500011
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11024
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[Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS LMB, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems,MOLECULAR AND CELLULAR PROBES,2013,27(41702):164-168
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