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Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems
[Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS LMB, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; cqhu@scsio.ac.cn
2013
Source PublicationMOLECULAR AND CELLULAR PROBES
ISSN0890-8508
Volume27Issue:41702Pages:164-168
AbstractVibrio alginolyticus has been confirmed as an important pathogen for aquatic animals. However, the pathogenic mechanism of V. alginolyticus is not completely understood. A total of 31 isolates of V. alginolyticus from sea water, fish and shrimp on the mariculture systems were fingerprinted by pulsed-field gel electrophoresis. The pathogenicity of these isolates was tested by challenge and the 21 genes associated with the virulence of Vibrio cholerae or Vibrio parahaemolyticus were examined in V. alginolyticus using PCR. The results showed that the 31 V. alginolyticus isolates belonged to 26 PFGE genotypes and the isolates from different source had different genotypes. Nine of the 31 isolates were confirmed as pathogenic strains by challenge. Moreover, 12 vibrio virulence genes were detected in this study. Of the detected genes, VCtoxR, VCtoxS, hlyA, VPtoxR and tlh were found in both pathogenic and non-pathogenic isolates. However, the other 7 virulence genes, ctxB, zot, tagA, stn, sto, tdh and trh, were only present in pathogenic isolates. Analysis of the relationship between virulence associated genes and pathogenicity of V. alginolyticus provides a possible explanation that the pathogenic mechanism of V. alginolyticus might be similar to that of V. parahaemolyticus instead of V. cholerae. (c) 2013 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordVibrio Alginolyticus Challenge Pathogenicity Virulence Associated Genes Detect Relationship
Subject AreaBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Cell Biology
Funding OrganizationThis study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02). ; This study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02). ; This study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02). ; This study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02).
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Language英语
Funding OrganizationThis study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02). ; This study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02). ; This study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02). ; This study was funded by Chinese Academy of Sciences Program (KSCX2-YW-N-089), Microbiological Network Lab of Chinese Academy of Sciences Program: epidemiology and control of the marine microbiology, and the National Key Technologies R&D Program (2009BAB44B02).
WOS IDWOS:000318961500011
Citation statistics
Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11024
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcqhu@scsio.ac.cn
Recommended Citation
GB/T 7714
[Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS LMB, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China,cqhu@scsio.ac.cn. Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems[J]. MOLECULAR AND CELLULAR PROBES,2013,27(41702):164-168.
APA [Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS LMB, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,Chinese Acad Sci, Chinese Acad Sci LAMB, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China,&cqhu@scsio.ac.cn.(2013).Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems.MOLECULAR AND CELLULAR PROBES,27(41702),164-168.
MLA [Hu, Chaoqun] Chinese Acad Sci, Key Lab Appl Marine Biol Guangdong Prov, CAS LMB, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,et al."Distribution and pathogenic relationship of virulence associated genes among Vibrio alginolyticus from the mariculture systems".MOLECULAR AND CELLULAR PROBES 27.41702(2013):164-168.
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