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Kinetic study of alkaline protease 894 for the hydrolysis of the pearl oyster Pinctada martensii
[Chen Xin; Liu Qingqin] Foshan Univ, Sch Sci, Foshan 528000, Peoples R China; [Chen Hua; Cai Bingna; Sun Huili] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Cai Bingna; Sun Huili] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; shl@scsio.ac.cn
2013
Source PublicationCHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN0254-4059
Volume31Issue:3Pages:591-597
AbstractA new enzyme (alkaline protease 894) obtained from the marine extremophile Flavobacterium yellowsea (YS-80-122) has exhibited strong substrate-binding and catalytic activity, even at low temperature, but the characteristics of the hydrolysis with this enzyme are still unclear. The pearl oyster Pinctada martensii was used in this study as the raw material to illustrate the kinetic properties of protease 894. After investigating the intrinsic relationship between the degree of hydrolysis and several factors, including initial reaction pH, temperature, substrate concentration, enzyme concentration, and hydrolysis time, the kinetics model was established. This study showed that the optimal conditions for the enzymatic hydrolysis were an initial reaction pH of 5.0, temperature of 30A degrees C, substrate concentration of 10% (w/v), enzyme concentration of 2 500 U/g, and hydrolysis time of 160 min. The kinetic characteristics of the protease for the hydrolysis of P. martensii were obtained. The inactivation constant was found to be 15.16/min, and the average relative error between the derived kinetics model and the actual measurement was only 3.04%, which indicated a high degree of fitness. Therefore, this study provides a basis for the investigation of the concrete kinetic characteristics of the new protease, which has potential applications in the food industry.
DepartmentLMB
KeywordAlkaline Protease 894 Pinctada Martensii Kinetics Model Inactivation Constant Proteolysis Rate Degree Of Hydrolysis
Subject AreaLimnology ; Oceanography
Funding OrganizationSupported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214) ; Supported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214) ; Supported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214) ; Supported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214)
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Language英语
Funding OrganizationSupported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214) ; Supported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214) ; Supported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214) ; Supported by the Comprehensive Strategic Cooperation Programs between Guangdong Province and Chinese Academy of Sciences (No. 2011A090100008), and the Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-EW-Q214)
WOS IDWOS:000318719700013
Citation statistics
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11029
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorshl@scsio.ac.cn
Recommended Citation
GB/T 7714
[Chen Xin,Liu Qingqin] Foshan Univ, Sch Sci, Foshan 528000, Peoples R China,[Chen Hua,et al. Kinetic study of alkaline protease 894 for the hydrolysis of the pearl oyster Pinctada martensii[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2013,31(3):591-597.
APA [Chen Xin.,Liu Qingqin] Foshan Univ, Sch Sci, Foshan 528000, Peoples R China.,[Chen Hua.,Cai Bingna.,Sun Huili] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.,...&shl@scsio.ac.cn.(2013).Kinetic study of alkaline protease 894 for the hydrolysis of the pearl oyster Pinctada martensii.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,31(3),591-597.
MLA [Chen Xin,et al."Kinetic study of alkaline protease 894 for the hydrolysis of the pearl oyster Pinctada martensii".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 31.3(2013):591-597.
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