Vibrio zhuhaiensis sp nov., isolated from a Japanese prawn (Marsupenaeus japonicus)
[Jin, Chunying] Huaqiao Univ, Coll Chem Engn, Xiamen 361021, Peoples R China; [Luo, Peng] South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Zuo, Huali; Chen, Jianming; Chen, Mingliang; Wang, Wei] State Ocean Adm, Key Lab Marine Biogenet Resources, Inst Oceanog 3, Xiamen 361005, Peoples R China; jcying@hqu.edu.cn; wangw.sz@gmail.com
2013
发表期刊ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN0003-6072
卷号103期号:5页码:989-996
摘要A Gram-negative, oxidase-positive, facultatively anaerobic bacterium, designated strain E20121, was isolated from the digestive tract of a Japanese prawn (Marsupenaeus japonicus) collected from the coastal sea water area of Zhuhai, Guangdong province, China. The new isolate was determined to be closely related to Vibrio ponticus DSM 16217(T), having 97.6 % 16S rRNA gene sequence similarity. Phylogenetic analysis based on recA, pyrH and rpoA also showed low levels of sequence similarities (72.6-96.6 %) with all species of the genus Vibrio. A multigene phylogenetic tree using concatenated sequences of the four genes (16S rRNA, rpoA, recA and pyrH) clearly showed that the new isolate is different from the currently known Vibrio species. DNA-DNA hybridization experiments revealed similarity values below 70 % with the closest related species V. ponticus DSM 16217(T). Several phenotypic traits enabled the differentiation of strain E20121 from the closest phylogenetic neighbours. The DNA G+C content of strain E20121 was determined to be 47.6 mol % and the major fatty acid components identified were C-16:1 omega 7c and/or C-16:1 omega 6c (39.8 %), C-18:1 omega 7c (13.6 %) and C-16:0 (9.6 %). Based on genotypic, phenotypic, chemotaxonomic, phylogenetic and DNA-DNA hybridization analyses, strain E20121 is proposed to represent a novel species of the genus Vibrio for which the name Vibrio zhuhaiensis sp. nov. is proposed. The type strain is E20121(T) (=DSM25602(T) = CCTCC AB 2011174(T)).
部门归属LMB
关键词Vibrio Zhuhaiensis Sp Nov. Polyphasic Taxonomy 16s Rrna
学科领域Microbiology
资助者This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance. ; This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance. ; This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance. ; This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance.
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语种英语
资助者This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance. ; This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance. ; This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance. ; This investigation was supported by Natural Science Foundation of China (31272684), China Ocean Mineral Resources R & D Association (DY-125-15-T-08), Natural Science Foundation of Fujian Province (2010J05074), the National Key Technology. R&D Program (2009BAB44B02) and the Frontier Key Program for Youngsters in SCSIO (SQ200801). We are grateful to Ms Li Gu for her excellent technical assistance.
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11033
专题中科院海洋生物资源可持续利用重点实验室
通讯作者jcying@hqu.edu.cn; wangw.sz@gmail.com
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[Jin, Chunying] Huaqiao Univ, Coll Chem Engn, Xiamen 361021, Peoples R China,[Luo, Peng] South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,[Zuo, Huali,et al. Vibrio zhuhaiensis sp nov., isolated from a Japanese prawn (Marsupenaeus japonicus)[J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,2013,103(5):989-996.
APA [Jin, Chunying] Huaqiao Univ, Coll Chem Engn, Xiamen 361021, Peoples R China.,[Luo, Peng] South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.,[Zuo, Huali.,Chen, Jianming.,Chen, Mingliang.,...&wangw.sz@gmail.com.(2013).Vibrio zhuhaiensis sp nov., isolated from a Japanese prawn (Marsupenaeus japonicus).ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,103(5),989-996.
MLA [Jin, Chunying] Huaqiao Univ, Coll Chem Engn, Xiamen 361021, Peoples R China,et al."Vibrio zhuhaiensis sp nov., isolated from a Japanese prawn (Marsupenaeus japonicus)".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 103.5(2013):989-996.
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