Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana
[Campbell, Douglas A.; Cockshutt, Amanda M.; Zhaxybayeva, Olga; Wu, Hongyan; Li, Gang] Mt Allison Univ, Dept Chem & Biochem, Sackville, NB E4L 1G7, Canada; [Hossain, Zakir; Cockshutt, Amanda M.] Environm Prote NB Inc, Sackville, NB E4L 3M7, Canada; [Zhaxybayeva, Olga] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA; [Wu, Hongyan] Hubei Univ Technol, Coll Biol Engn, Wuhan 430068, Hubei, Peoples R China; [Li, Gang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; dcampbell@mta.ca
2013
发表期刊PHOTOSYNTHESIS RESEARCH
ISSN0166-8595
卷号115期号:1页码:43-54
摘要All oxygenic photoautotrophs suffer photoinactivation of their Photosystem II complexes, at a rate driven by the instantaneous light level. To maintain photosynthesis, PsbA subunits are proteolytically removed from photoinactivated Photosystem II complexes, primarily by a membrane-bound FtsH protease. Diatoms thrive in environments with fluctuating light, such as coastal regions, in part because they enjoy a low susceptibility to photoinactivation of Photosystem II. In a coastal strain of the diatom Thalassiosira pseudonana growing across a range of light levels, active Photosystem II represents only about 42 % of the total Photosystem II protein, with the remainder attributable to photoinactivated Photosystem II awaiting recycling. The rate constant for removal of PsbA protein increases with growth light, in parallel with an increasing content of the FtsH protease relative to the substrate PsbA. An offshore strain of Thalassiosira pseudonana, originating from a more stable light environment, had a lower content of FtsH and slower rate constants for removal of PsbA. We used this data to generate the first estimates for in vivo proteolytic degradation of photoinactivated PsbA per FtsH(6) protease, at similar to 3.9 x 10(-2) s(-1), which proved consistent across growth lights and across the onshore and offshore strains.
部门归属LMB
关键词Diatom D1 D2 Ftsh Photoinactivation Photosystem Ii Psba Psbd
学科领域Plant Sciences
资助者We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts.
URL查看原文
语种英语
资助者We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts.
WOS记录号WOS:000319016700003
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11034
专题中科院海洋生物资源可持续利用重点实验室
通讯作者dcampbell@mta.ca
推荐引用方式
GB/T 7714
[Campbell, Douglas A.,Cockshutt, Amanda M.,Zhaxybayeva, Olga,et al. Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana[J]. PHOTOSYNTHESIS RESEARCH,2013,115(1):43-54.
APA [Campbell, Douglas A..,Cockshutt, Amanda M..,Zhaxybayeva, Olga.,Wu, Hongyan.,Li, Gang] Mt Allison Univ, Dept Chem & Biochem, Sackville, NB E4L 1G7, Canada.,...&dcampbell@mta.ca.(2013).Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana.PHOTOSYNTHESIS RESEARCH,115(1),43-54.
MLA [Campbell, Douglas A.,et al."Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana".PHOTOSYNTHESIS RESEARCH 115.1(2013):43-54.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Photosystem II prote(435KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。