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Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana
[Campbell, Douglas A.; Cockshutt, Amanda M.; Zhaxybayeva, Olga; Wu, Hongyan; Li, Gang] Mt Allison Univ, Dept Chem & Biochem, Sackville, NB E4L 1G7, Canada; [Hossain, Zakir; Cockshutt, Amanda M.] Environm Prote NB Inc, Sackville, NB E4L 3M7, Canada; [Zhaxybayeva, Olga] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA; [Wu, Hongyan] Hubei Univ Technol, Coll Biol Engn, Wuhan 430068, Hubei, Peoples R China; [Li, Gang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; dcampbell@mta.ca
2013
Source PublicationPHOTOSYNTHESIS RESEARCH
ISSN0166-8595
Volume115Issue:1Pages:43-54
AbstractAll oxygenic photoautotrophs suffer photoinactivation of their Photosystem II complexes, at a rate driven by the instantaneous light level. To maintain photosynthesis, PsbA subunits are proteolytically removed from photoinactivated Photosystem II complexes, primarily by a membrane-bound FtsH protease. Diatoms thrive in environments with fluctuating light, such as coastal regions, in part because they enjoy a low susceptibility to photoinactivation of Photosystem II. In a coastal strain of the diatom Thalassiosira pseudonana growing across a range of light levels, active Photosystem II represents only about 42 % of the total Photosystem II protein, with the remainder attributable to photoinactivated Photosystem II awaiting recycling. The rate constant for removal of PsbA protein increases with growth light, in parallel with an increasing content of the FtsH protease relative to the substrate PsbA. An offshore strain of Thalassiosira pseudonana, originating from a more stable light environment, had a lower content of FtsH and slower rate constants for removal of PsbA. We used this data to generate the first estimates for in vivo proteolytic degradation of photoinactivated PsbA per FtsH(6) protease, at similar to 3.9 x 10(-2) s(-1), which proved consistent across growth lights and across the onshore and offshore strains.
DepartmentLMB
KeywordDiatom D1 D2 Ftsh Photoinactivation Photosystem Ii Psba Psbd
Subject AreaPlant Sciences
Funding OrganizationWe thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts.
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Language英语
Funding OrganizationWe thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts. ; We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts.
WOS IDWOS:000319016700003
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11034
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authordcampbell@mta.ca
Recommended Citation
GB/T 7714
[Campbell, Douglas A.,Cockshutt, Amanda M.,Zhaxybayeva, Olga,et al. Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana[J]. PHOTOSYNTHESIS RESEARCH,2013,115(1):43-54.
APA [Campbell, Douglas A..,Cockshutt, Amanda M..,Zhaxybayeva, Olga.,Wu, Hongyan.,Li, Gang] Mt Allison Univ, Dept Chem & Biochem, Sackville, NB E4L 1G7, Canada.,...&dcampbell@mta.ca.(2013).Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana.PHOTOSYNTHESIS RESEARCH,115(1),43-54.
MLA [Campbell, Douglas A.,et al."Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana".PHOTOSYNTHESIS RESEARCH 115.1(2013):43-54.
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