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学科主题: Plant Sciences
题名: Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana
作者: [Campbell, Douglas A. ; Cockshutt, Amanda M. ; Zhaxybayeva, Olga ; Wu, Hongyan ; Li, Gang] Mt Allison Univ, Dept Chem & Biochem, Sackville, NB E4L 1G7, Canada ; [Hossain, Zakir ; Cockshutt, Amanda M.] Environm Prote NB Inc, Sackville, NB E4L 3M7, Canada ; [Zhaxybayeva, Olga] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA ; [Wu, Hongyan] Hubei Univ Technol, Coll Biol Engn, Wuhan 430068, Hubei, Peoples R China ; [Li, Gang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
通讯作者: dcampbell@mta.ca
关键词: Diatom D1 D2 ; FtsH ; Photoinactivation ; Photosystem II ; PsbA PsbD
刊名: PHOTOSYNTHESIS RESEARCH
发表日期: 2013
卷: 115, 期:1, 页:43-54
部门归属: LMB
资助者: We thank J. Jeans for assistance with protein quantitations and K. Dezeeuw for literature searches on FtsH. This study was supported by NSERC Discovery and Canada Research Chair funding to D. A. C; and by MITACS Post-doctoral support (O.Z.). Antibodies and protein standards were supplied by AgriSera AB and Environmental Proteomics NB. D. A. C. thanks Dr. Adrian K. Clarke for an invitation to a protease workshop in 2002 which inspired these efforts.
摘要: All oxygenic photoautotrophs suffer photoinactivation of their Photosystem II complexes, at a rate driven by the instantaneous light level. To maintain photosynthesis, PsbA subunits are proteolytically removed from photoinactivated Photosystem II complexes, primarily by a membrane-bound FtsH protease. Diatoms thrive in environments with fluctuating light, such as coastal regions, in part because they enjoy a low susceptibility to photoinactivation of Photosystem II. In a coastal strain of the diatom Thalassiosira pseudonana growing across a range of light levels, active Photosystem II represents only about 42 % of the total Photosystem II protein, with the remainder attributable to photoinactivated Photosystem II awaiting recycling. The rate constant for removal of PsbA protein increases with growth light, in parallel with an increasing content of the FtsH protease relative to the substrate PsbA. An offshore strain of Thalassiosira pseudonana, originating from a more stable light environment, had a lower content of FtsH and slower rate constants for removal of PsbA. We used this data to generate the first estimates for in vivo proteolytic degradation of photoinactivated PsbA per FtsH(6) protease, at similar to 3.9 x 10(-2) s(-1), which proved consistent across growth lights and across the onshore and offshore strains.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000319016700003
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11034
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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[Campbell, Douglas A.; Cockshutt, Amanda M.; Zhaxybayeva, Olga; Wu, Hongyan; Li, Gang] Mt Allison Univ, Dept Chem & Biochem, Sackville, NB E4L 1G7, Canada; [Hossain, Zakir; Cockshutt, Amanda M.] Environm Prote NB Inc, Sackville, NB E4L 3M7, Canada; [Zhaxybayeva, Olga] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA; [Wu, Hongyan] Hubei Univ Technol, Coll Biol Engn, Wuhan 430068, Hubei, Peoples R China; [Li, Gang] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China.Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana,PHOTOSYNTHESIS RESEARCH,2013,115(1):43-54
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