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iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin
[Han, Zhuang; He, Fei; Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; Zhang, Yu; Xu, Ying; Matsumura, Kiyotaka; He, Li-Sheng; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China; [Han, Zhuang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Sun, Jin; Qiu, Jian-Wen] Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China; [Zhang, Yu] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen, Peoples R China; boqianpy@ust.hk
2013
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume12Issue:5Pages:2090-2100
AbstractMarine biofouling refers to the unwanted accumulation of fouling organisms such as barnacles, on artificial surfaces, resulting in severe consequences for marine industries. Meleagrin is a potential nontoxic antifoulant that is isolated from the fungus Penicillium sp.; however, its mechanistic effect mode of action On larval settlement remains unknown. Here, we applied iTRAQ coupled with 2D LC-MS/MS proteomic analysis to investigate the effect of meleagrin on the proteomic expression profile of cyprid development and aging in the barnacle Balanus amphitrite. Fifty proteins were differentially expressed in response to treatment with meleagrin, among which 26 proteins were associated with cyprid development/aging and 24 were specifically associated with the meleagrin treatment. The 66 proteins that were associated with aging only remained unaltered during exposure to meleagrin. Using KEGG analysis, those proteins were assigned to several groups, including metabolic pathways, ECM-receptor interactions, and the regulation of the actin cytoskeleton. Among the 24 proteins that were not related to the development/aging process, expression of the cyprid major protein (CMP), a vitellogenin-like protein, increased after the meleagrin treatment, which suggested that meleagrin might affect the endocrine system and prevent the larval molting cycle. With the exception of the chitin binding protein that mediates the molting process and ATPase-mediated energy processes, the majority of proteins with significant effects in previous studies in response to cyprid treatment with butenolide and polyether B remained unchanged in the present study, suggesting that meleagrin may exhibit a different mechanism.
DepartmentLMB
KeywordBarnacle Larval Settlement Antifouling Meleagrin Itraq Proteome Biofouling
Subject AreaBiochemical Research Methods
Funding OrganizationThis work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian.
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Language英语
Funding OrganizationThis work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian.
WOS IDWOS:000318589000008
Citation statistics
Cited Times:31[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11035
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorboqianpy@ust.hk
Recommended Citation
GB/T 7714
[Han, Zhuang,He, Fei,Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,et al. iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(5):2090-2100.
APA [Han, Zhuang.,He, Fei.,Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.,Zhang, Yu.,Xu, Ying.,...&boqianpy@ust.hk.(2013).iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin.JOURNAL OF PROTEOME RESEARCH,12(5),2090-2100.
MLA [Han, Zhuang,et al."iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin".JOURNAL OF PROTEOME RESEARCH 12.5(2013):2090-2100.
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