iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin
[Han, Zhuang; He, Fei; Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; Zhang, Yu; Xu, Ying; Matsumura, Kiyotaka; He, Li-Sheng; Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China; [Han, Zhuang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Sun, Jin; Qiu, Jian-Wen] Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China; [Zhang, Yu] Shenzhen Univ, Coll Life Sci, Shenzhen Key Lab Marine Bioresource & Ecoenvironm, Shenzhen, Peoples R China; boqianpy@ust.hk
2013
发表期刊JOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
卷号12期号:5页码:2090-2100
摘要Marine biofouling refers to the unwanted accumulation of fouling organisms such as barnacles, on artificial surfaces, resulting in severe consequences for marine industries. Meleagrin is a potential nontoxic antifoulant that is isolated from the fungus Penicillium sp.; however, its mechanistic effect mode of action On larval settlement remains unknown. Here, we applied iTRAQ coupled with 2D LC-MS/MS proteomic analysis to investigate the effect of meleagrin on the proteomic expression profile of cyprid development and aging in the barnacle Balanus amphitrite. Fifty proteins were differentially expressed in response to treatment with meleagrin, among which 26 proteins were associated with cyprid development/aging and 24 were specifically associated with the meleagrin treatment. The 66 proteins that were associated with aging only remained unaltered during exposure to meleagrin. Using KEGG analysis, those proteins were assigned to several groups, including metabolic pathways, ECM-receptor interactions, and the regulation of the actin cytoskeleton. Among the 24 proteins that were not related to the development/aging process, expression of the cyprid major protein (CMP), a vitellogenin-like protein, increased after the meleagrin treatment, which suggested that meleagrin might affect the endocrine system and prevent the larval molting cycle. With the exception of the chitin binding protein that mediates the molting process and ATPase-mediated energy processes, the majority of proteins with significant effects in previous studies in response to cyprid treatment with butenolide and polyether B remained unchanged in the present study, suggesting that meleagrin may exhibit a different mechanism.
部门归属LMB
关键词Barnacle Larval Settlement Antifouling Meleagrin Itraq Proteome Biofouling
学科领域Biochemical Research Methods
资助者This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian.
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语种英语
资助者This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian. ; This work was supported by a grant (DY125-15-T-02) from the China Ocean Mineral Resources Research and Development Association, a joint research grant from the Research Grants Council of the HKSAR and the National Natural Science Foundation of China (N_HKUST602/09), and award (SA-C0040/UK-C0016) from King Abdullah University of Science and Technology to P.Y. Qian.
WOS记录号WOS:000318589000008
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11035
专题中科院海洋生物资源可持续利用重点实验室
通讯作者boqianpy@ust.hk
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GB/T 7714
[Han, Zhuang,He, Fei,Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,et al. iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin[J]. JOURNAL OF PROTEOME RESEARCH,2013,12(5):2090-2100.
APA [Han, Zhuang.,He, Fei.,Qi, Shu-Hua] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.,Zhang, Yu.,Xu, Ying.,...&boqianpy@ust.hk.(2013).iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin.JOURNAL OF PROTEOME RESEARCH,12(5),2090-2100.
MLA [Han, Zhuang,et al."iTRAQ-Based Proteomic Profiling of the Barnacle Balanus amphitrite in Response to the Antifouling Compound Meleagrin".JOURNAL OF PROTEOME RESEARCH 12.5(2013):2090-2100.
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