SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization
[Wu, Shan-Zeng; Huang, Xian-De; Li, Qin; He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; hmx@scsio.ac.cn
2013
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume34Issue:5Pages:1050-1056
AbstractIL-17 from pearl oyster Pinctada fucata, one of mollusk, was identified and characterized, and its genomic structure and promoter were analyzed. The full-length cDNA of P. fucata IL-17 (PfIL-17) is 907 bp with an open reading frame of 585 bp encoding a putative protein of 194 amino acids. The deduced PfIL-17 contains a 19 amino acid signal peptide and a conserved IL-17 domain. Multiple sequence alignments and phylogenetic analysis revealed that PfIL-17 has lower similarity with other invertebrate IL-17 and was clustered with CgIL-17, but not clustered with other invertebrate IL-17. Gene expression analysis indicated that PfIL-17 took part in the immune response to LPS and poly(I:C) stimulation, and dual-luciferase reporter assays showed that PfIL-17 could active vertebrate target genes containing the NF-kappa B binding site and involve NF-kappa B signal pathway in HEK293 cells. Combined with the results mentioned above, it is suggested that PfIL-17 might involve and activate NF-kappa B signal pathway against extracellular pathogens. (C) 2013 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordPearl Oyster Pfil-17 Gene Cloning Immune Response
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationThe pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145).
URL查看原文
Language英语
Funding OrganizationThe pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145).
WOS IDWOS:000317870600004
Citation statistics
Cited Times:28[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11037
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhmx@scsio.ac.cn
Recommended Citation
GB/T 7714
[Wu, Shan-Zeng,Huang, Xian-De,Li, Qin,et al. Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1050-1056.
APA [Wu, Shan-Zeng,Huang, Xian-De,Li, Qin,He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,&hmx@scsio.ac.cn.(2013).Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization.FISH & SHELLFISH IMMUNOLOGY,34(5),1050-1056.
MLA [Wu, Shan-Zeng,et al."Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1050-1056.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.