Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization
[Wu, Shan-Zeng; Huang, Xian-De; Li, Qin; He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; hmx@scsio.ac.cn
2013
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号34期号:5页码:1050-1056
摘要IL-17 from pearl oyster Pinctada fucata, one of mollusk, was identified and characterized, and its genomic structure and promoter were analyzed. The full-length cDNA of P. fucata IL-17 (PfIL-17) is 907 bp with an open reading frame of 585 bp encoding a putative protein of 194 amino acids. The deduced PfIL-17 contains a 19 amino acid signal peptide and a conserved IL-17 domain. Multiple sequence alignments and phylogenetic analysis revealed that PfIL-17 has lower similarity with other invertebrate IL-17 and was clustered with CgIL-17, but not clustered with other invertebrate IL-17. Gene expression analysis indicated that PfIL-17 took part in the immune response to LPS and poly(I:C) stimulation, and dual-luciferase reporter assays showed that PfIL-17 could active vertebrate target genes containing the NF-kappa B binding site and involve NF-kappa B signal pathway in HEK293 cells. Combined with the results mentioned above, it is suggested that PfIL-17 might involve and activate NF-kappa B signal pathway against extracellular pathogens. (C) 2013 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Pearl Oyster Pfil-17 Gene Cloning Immune Response
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145).
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语种英语
资助者The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145).
WOS记录号WOS:000317870600004
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被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11037
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx@scsio.ac.cn
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GB/T 7714
[Wu, Shan-Zeng,Huang, Xian-De,Li, Qin,et al. Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1050-1056.
APA [Wu, Shan-Zeng,Huang, Xian-De,Li, Qin,He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,&hmx@scsio.ac.cn.(2013).Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization.FISH & SHELLFISH IMMUNOLOGY,34(5),1050-1056.
MLA [Wu, Shan-Zeng,et al."Interleukin-17 in pearl oyster (Pinctada fucata): Molecular cloning and functional characterization".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1050-1056.
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