Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas
[Zhang, Yang; Li, Jun; Yu, Feng; He, Xiaocui; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; carlzyu@scsio.ac.cn
2013
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号34期号:5页码:1071-1077
摘要Allograft inflammatory factor-1 (AIF-1) is a calcium-binding cytokine associated with immune cell activation and inflammatory response. Presently, we have identified and characterized an AIF-1 in a marine bivalve mollusk, Crassostrea gigas, and designated it as CgAIF-1. The full-length CgAIF-1 cDNA is 794 bp, encoding a protein of 149 amino acids with two conserved EF hand Ca2+-binding motifs. CgAIF-1 is constitutively expressed in various tissues with enriched expression in hemocytes. Moreover, CgAIF-1 transcription is induced by multiple Pathogen-Associated Molecular Patterns (PAMPs), including poly (I: C), LPS, PGN, HKLM and HKVA, but is limited by 1,3-beta-glucan. Furthermore, recombinant CgAIF-1 can specifically stimulate phagocytic ability of granulocytes, but not of intermediate cells and hyalinocytes. CgAIF-1 also enhances mRNA levels of MIF, TNF and IL-17. These results provide the first functional evidence that CgAIF-1 is involved in hemocyte activation in C gigas, revealing conserved functions of AIF-1 in host defense from mollusks to mammals. (C) 2013 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Crassostrea Gigas Aif-1 Hemocyte Activation Phagocytosis Inflammatory Cytokine
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript.
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资助者This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript.
WOS记录号WOS:000317870600007
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被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11038
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
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GB/T 7714
[Zhang, Yang,Li, Jun,Yu, Feng,et al. Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1071-1077.
APA [Zhang, Yang,Li, Jun,Yu, Feng,He, Xiaocui,Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,&carlzyu@scsio.ac.cn.(2013).Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,34(5),1071-1077.
MLA [Zhang, Yang,et al."Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1071-1077.
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