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Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas
[Zhang, Yang; Li, Jun; Yu, Feng; He, Xiaocui; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; carlzyu@scsio.ac.cn
2013
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume34Issue:5Pages:1071-1077
AbstractAllograft inflammatory factor-1 (AIF-1) is a calcium-binding cytokine associated with immune cell activation and inflammatory response. Presently, we have identified and characterized an AIF-1 in a marine bivalve mollusk, Crassostrea gigas, and designated it as CgAIF-1. The full-length CgAIF-1 cDNA is 794 bp, encoding a protein of 149 amino acids with two conserved EF hand Ca2+-binding motifs. CgAIF-1 is constitutively expressed in various tissues with enriched expression in hemocytes. Moreover, CgAIF-1 transcription is induced by multiple Pathogen-Associated Molecular Patterns (PAMPs), including poly (I: C), LPS, PGN, HKLM and HKVA, but is limited by 1,3-beta-glucan. Furthermore, recombinant CgAIF-1 can specifically stimulate phagocytic ability of granulocytes, but not of intermediate cells and hyalinocytes. CgAIF-1 also enhances mRNA levels of MIF, TNF and IL-17. These results provide the first functional evidence that CgAIF-1 is involved in hemocyte activation in C gigas, revealing conserved functions of AIF-1 in host defense from mollusks to mammals. (C) 2013 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordCrassostrea Gigas Aif-1 Hemocyte Activation Phagocytosis Inflammatory Cytokine
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationThis work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript.
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Language英语
Funding OrganizationThis work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript. ; This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript.
WOS IDWOS:000317870600007
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11038
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcarlzyu@scsio.ac.cn
Recommended Citation
GB/T 7714
[Zhang, Yang,Li, Jun,Yu, Feng,et al. Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1071-1077.
APA [Zhang, Yang,Li, Jun,Yu, Feng,He, Xiaocui,Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,&carlzyu@scsio.ac.cn.(2013).Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,34(5),1071-1077.
MLA [Zhang, Yang,et al."Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1071-1077.
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