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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata
作者: [Huang, Xian-De ; Liu, Wen-Guang ; Wang, Qi ; Zhao, Mi ; Wu, Shan-Zeng ; Guan, Yun-Yan ; Shi, Yu ; He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
通讯作者: hmx@scsio.ac.cn
关键词: Pearl oyster ; PfIRF-2 ; Gene cloning ; Immune response
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2013
卷: 34, 期:5, 页:1279-1286
部门归属: LMB
资助者: The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001).
摘要: Interferon regulatory factors (IRFs) control many facets of the innate and adaptive immune responses, regulate the development of the immune system itself and involve in reproduction and morphogenesis. In the present study, the IRF-2 homology gene, PfIRF-2 from pearl oyster Pinctada fucata was cloned and its genomic structure and promoter were analyzed. PfIRF-2 encodes a putative protein of 350 amino acids, and contains a highly conserved N-terminal DNA-binding domain and a variable C-terminal regulatory domain. Comparison and phylogenetic analysis revealed that PfIRF-2 shared a relatively higher identity with other mollusk but relatively lower identity with vertebrate IRF-2, and was clustered with IRF-1 subfamily composed of IRF-2 and IRF-1. Furthermore, gene expression analysis revealed that PfIRF-2 involved in the immune response to LPS and poly(I:C) stimulation. Immunofluorescence assay showed that the expressed PfIRF-2 was translocated into the nucleus and dual-luciferase reporter assays indicated that PfIRF-2 could involved and activate interferon signaling or NF-kappa B signal pathway in HEK293 cells. The study of PfIRF-2 may help better understand the innate immune in mollusk. (C) 2013 Elsevier Ltd. All rights reserved.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000317870600032
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11040
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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[Huang, Xian-De; Liu, Wen-Guang; Wang, Qi; Zhao, Mi; Wu, Shan-Zeng; Guan, Yun-Yan; Shi, Yu; He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata,FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1279-1286
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文件名: Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata.pdf
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