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Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata
[Huang, Xian-De; Liu, Wen-Guang; Wang, Qi; Zhao, Mi; Wu, Shan-Zeng; Guan, Yun-Yan; Shi, Yu; He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; hmx@scsio.ac.cn
2013
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume34Issue:5Pages:1279-1286
AbstractInterferon regulatory factors (IRFs) control many facets of the innate and adaptive immune responses, regulate the development of the immune system itself and involve in reproduction and morphogenesis. In the present study, the IRF-2 homology gene, PfIRF-2 from pearl oyster Pinctada fucata was cloned and its genomic structure and promoter were analyzed. PfIRF-2 encodes a putative protein of 350 amino acids, and contains a highly conserved N-terminal DNA-binding domain and a variable C-terminal regulatory domain. Comparison and phylogenetic analysis revealed that PfIRF-2 shared a relatively higher identity with other mollusk but relatively lower identity with vertebrate IRF-2, and was clustered with IRF-1 subfamily composed of IRF-2 and IRF-1. Furthermore, gene expression analysis revealed that PfIRF-2 involved in the immune response to LPS and poly(I:C) stimulation. Immunofluorescence assay showed that the expressed PfIRF-2 was translocated into the nucleus and dual-luciferase reporter assays indicated that PfIRF-2 could involved and activate interferon signaling or NF-kappa B signal pathway in HEK293 cells. The study of PfIRF-2 may help better understand the innate immune in mollusk. (C) 2013 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordPearl Oyster Pfirf-2 Gene Cloning Immune Response
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationThe pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001).
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Language英语
Funding OrganizationThe pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001).
WOS IDWOS:000317870600032
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11040
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhmx@scsio.ac.cn
Recommended Citation
GB/T 7714
[Huang, Xian-De,Liu, Wen-Guang,Wang, Qi,et al. Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1279-1286.
APA [Huang, Xian-De.,Liu, Wen-Guang.,Wang, Qi.,Zhao, Mi.,Wu, Shan-Zeng.,...&hmx@scsio.ac.cn.(2013).Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata.FISH & SHELLFISH IMMUNOLOGY,34(5),1279-1286.
MLA [Huang, Xian-De,et al."Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1279-1286.
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