Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata
[Huang, Xian-De; Liu, Wen-Guang; Wang, Qi; Zhao, Mi; Wu, Shan-Zeng; Guan, Yun-Yan; Shi, Yu; He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; hmx@scsio.ac.cn
2013
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号34期号:5页码:1279-1286
摘要Interferon regulatory factors (IRFs) control many facets of the innate and adaptive immune responses, regulate the development of the immune system itself and involve in reproduction and morphogenesis. In the present study, the IRF-2 homology gene, PfIRF-2 from pearl oyster Pinctada fucata was cloned and its genomic structure and promoter were analyzed. PfIRF-2 encodes a putative protein of 350 amino acids, and contains a highly conserved N-terminal DNA-binding domain and a variable C-terminal regulatory domain. Comparison and phylogenetic analysis revealed that PfIRF-2 shared a relatively higher identity with other mollusk but relatively lower identity with vertebrate IRF-2, and was clustered with IRF-1 subfamily composed of IRF-2 and IRF-1. Furthermore, gene expression analysis revealed that PfIRF-2 involved in the immune response to LPS and poly(I:C) stimulation. Immunofluorescence assay showed that the expressed PfIRF-2 was translocated into the nucleus and dual-luciferase reporter assays indicated that PfIRF-2 could involved and activate interferon signaling or NF-kappa B signal pathway in HEK293 cells. The study of PfIRF-2 may help better understand the innate immune in mollusk. (C) 2013 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Pearl Oyster Pfirf-2 Gene Cloning Immune Response
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001).
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语种英语
资助者The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the Funds of Knowledge Innovation Program of Chinese Academy of Sciences (ZCX2-EW-Q21), the National Science and Technology Program of China (2012AA10A410), the National Natural Science Foundation of China (41176145) and Joint program of NSFC-Guangdong (U0831001).
WOS记录号WOS:000317870600032
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11040
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx@scsio.ac.cn
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[Huang, Xian-De,Liu, Wen-Guang,Wang, Qi,et al. Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1279-1286.
APA [Huang, Xian-De.,Liu, Wen-Guang.,Wang, Qi.,Zhao, Mi.,Wu, Shan-Zeng.,...&hmx@scsio.ac.cn.(2013).Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata.FISH & SHELLFISH IMMUNOLOGY,34(5),1279-1286.
MLA [Huang, Xian-De,et al."Molecular characterization of interferon regulatory factor 2 (IRF-2) homolog in pearl oyster Pinctada fucata".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1279-1286.
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