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Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis
[Yin, Jianhua; Sun, Linlin; Dong, Yangyang; Chi, Xun; Gao, Haichun] Zhejiang Univ, Inst Microbiol, Hangzhou 310003, Zhejiang, Peoples R China; Gao, Haichun] Zhejiang Univ, Coll Life Sci, Hangzhou 310003, Zhejiang, Peoples R China; [Zhu, Weiming] Ocean Univ China, Sch Med & Pharm, Qingdao, Shandong, Peoples R China; [Qi, Shu-hua] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China; haichung@zju.edu.cn
2013
Source PublicationPLOS ONE
ISSN1932-6203
Volume8Issue:3Pages:-E-5275
AbstractShewanella oneidensis is a facultative anaerobic gamma-proteobacterium possessing remarkably diverse respiratory capacities for reducing various organic and inorganic substrates. As a veteran research model for investigating redox transformations of environmental contaminants the bacterium is well known to be a naturally ampicillin-resistant microorganism. However, in this study we discovered that ampicillin has a significant impact on growth of S. oneidensis. Particularly, cell lysis occurred only with ampicillin at levels ranging from 0.49 to 6.25 mu g/ml but not at 50 mu g/ml. This phenotype is attributable to insufficient expression of the beta-lactamase BlaA. The subsequent analysis revealed that the blaA gene is strongly induced by ampicillin at high (50 mu g/ml), but not at low levels (2.5 mu g/ml). In addition, we demonstrated that penicillin binding protein 5 (PBP5), the most abundant low molecular weight PBP (LMW PBP), is the only one relevant to beta-lactam resistance under the tested conditions. This nonessential PBP, largely resembling its Escherichia coli counterpart in functionality, mediates expression of the blaA gene.
DepartmentLMB
Subject AreaMultidisciplinary Sciences
Funding OrganizationThis research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Language英语
Funding OrganizationThis research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. ; This research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS IDWOS:000317262200117
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11050
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhaichung@zju.edu.cn
Recommended Citation
GB/T 7714
[Yin, Jianhua,Sun, Linlin,Dong, Yangyang,et al. Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis[J]. PLOS ONE,2013,8(3):-E-5275.
APA [Yin, Jianhua.,Sun, Linlin.,Dong, Yangyang.,Chi, Xun.,Gao, Haichun] Zhejiang Univ, Inst Microbiol, Hangzhou 310003, Zhejiang, Peoples R China.,...&haichung@zju.edu.cn.(2013).Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis.PLOS ONE,8(3),-E-5275.
MLA [Yin, Jianhua,et al."Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis".PLOS ONE 8.3(2013):-E-5275.
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