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学科主题: Multidisciplinary Sciences
题名: Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis
作者: [Yin, Jianhua ; Sun, Linlin ; Dong, Yangyang ; Chi, Xun ; Gao, Haichun] Zhejiang Univ, Inst Microbiol, Hangzhou 310003, Zhejiang, Peoples R China ; [Yin, Jianhua ; Sun, Linlin ; Dong, Yangyang ; Chi, Xun ; Gao, Haichun] Zhejiang Univ, Coll Life Sci, Hangzhou 310003, Zhejiang, Peoples R China ; [Zhu, Weiming] Ocean Univ China, Sch Med & Pharm, Qingdao, Shandong, Peoples R China ; [Qi, Shu-hua] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
通讯作者: haichung@zju.edu.cn
刊名: PLOS ONE
发表日期: 2013
卷: 8, 期:3, 页:-E-5275
部门归属: LMB
资助者: This research was supported by Major State Basic Research Development Program (973 Program: 2010CB833803), National Natural Science Foundation of China (31270097), and Natural Science Foundation of Zhejiang province (R3110096) and Major Program of Science and Technology Department of Zhejiang (2009C12061) to HG. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
摘要: Shewanella oneidensis is a facultative anaerobic gamma-proteobacterium possessing remarkably diverse respiratory capacities for reducing various organic and inorganic substrates. As a veteran research model for investigating redox transformations of environmental contaminants the bacterium is well known to be a naturally ampicillin-resistant microorganism. However, in this study we discovered that ampicillin has a significant impact on growth of S. oneidensis. Particularly, cell lysis occurred only with ampicillin at levels ranging from 0.49 to 6.25 mu g/ml but not at 50 mu g/ml. This phenotype is attributable to insufficient expression of the beta-lactamase BlaA. The subsequent analysis revealed that the blaA gene is strongly induced by ampicillin at high (50 mu g/ml), but not at low levels (2.5 mu g/ml). In addition, we demonstrated that penicillin binding protein 5 (PBP5), the most abundant low molecular weight PBP (LMW PBP), is the only one relevant to beta-lactam resistance under the tested conditions. This nonessential PBP, largely resembling its Escherichia coli counterpart in functionality, mediates expression of the blaA gene.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000317262200117
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11050
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
[Yin, Jianhua; Sun, Linlin; Dong, Yangyang; Chi, Xun; Gao, Haichun] Zhejiang Univ, Inst Microbiol, Hangzhou 310003, Zhejiang, Peoples R China; [Yin, Jianhua; Sun, Linlin; Dong, Yangyang; Chi, Xun; Gao, Haichun] Zhejiang Univ, Coll Life Sci, Hangzhou 310003, Zhejiang, Peoples R China; [Zhu, Weiming] Ocean Univ China, Sch Med & Pharm, Qingdao, Shandong, Peoples R China; [Qi, Shu-hua] Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China.Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis,PLOS ONE,2013,8(3):-E-5275
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