Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete
[Mai, Zhimao; Yang, Jian; Tian, Xinpeng; Li, Jie; Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China; Zhang, Si] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; zhsimd@scsio.ac.cn
2013
发表期刊APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
卷号169期号:5页码:1512-1522
摘要The gene BglNH encoding a beta-glucosidase was cloned from a marine streptomycete. Sequence analysis revealed that BglNH encoded a 456-aa peptide with a calculated mass of 51 kDa. The deduced amino acid sequence of BglNH showed the highest identities of 61 % with known beta-glucosidases and contained a catalytic domain which belonged to the glycoside hydrolase family 1. The gene BglNH was expressed in Escherichia coli and the recombinant enzyme (r-BglNH) was purified. The optimum pH and temperature of r-BglNH were pH 6.0 and 45 A degrees C, respectively. The r-BglNH displayed the typical salt-tolerant and glucose-enhanced characteristics. Its activity was remarkably enhanced in the presence of 0.5 M NaCl (rose more than 1.6-fold) and 0.1 M glucose (rose more than 1.4-fold). Moreover, r-BglNH displayed good pH stability and metal tolerance. It remained stable after incubating with buffers from pH 4.0 to 10.0, and most metal ions had no significant inhibition on its activity. These properties indicate that r-BglNH is an ideal candidate for further research and industrial applications.
部门归属LMB
关键词Beta-glucosidase Cellulase Salt-tolerant Glucose-enhanced Streptomyces Sp
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
资助者This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104).
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资助者This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104).
WOS记录号WOS:000316116500004
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11060
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zhsimd@scsio.ac.cn
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[Mai, Zhimao,Yang, Jian,Tian, Xinpeng,et al. Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,169(5):1512-1522.
APA [Mai, Zhimao.,Yang, Jian.,Tian, Xinpeng.,Li, Jie.,Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China.,...&zhsimd@scsio.ac.cn.(2013).Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,169(5),1512-1522.
MLA [Mai, Zhimao,et al."Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 169.5(2013):1512-1522.
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