SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete
[Mai, Zhimao; Yang, Jian; Tian, Xinpeng; Li, Jie; Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China; Zhang, Si] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; zhsimd@scsio.ac.cn
2013
Source PublicationAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
ISSN0273-2289
Volume169Issue:5Pages:1512-1522
AbstractThe gene BglNH encoding a beta-glucosidase was cloned from a marine streptomycete. Sequence analysis revealed that BglNH encoded a 456-aa peptide with a calculated mass of 51 kDa. The deduced amino acid sequence of BglNH showed the highest identities of 61 % with known beta-glucosidases and contained a catalytic domain which belonged to the glycoside hydrolase family 1. The gene BglNH was expressed in Escherichia coli and the recombinant enzyme (r-BglNH) was purified. The optimum pH and temperature of r-BglNH were pH 6.0 and 45 A degrees C, respectively. The r-BglNH displayed the typical salt-tolerant and glucose-enhanced characteristics. Its activity was remarkably enhanced in the presence of 0.5 M NaCl (rose more than 1.6-fold) and 0.1 M glucose (rose more than 1.4-fold). Moreover, r-BglNH displayed good pH stability and metal tolerance. It remained stable after incubating with buffers from pH 4.0 to 10.0, and most metal ions had no significant inhibition on its activity. These properties indicate that r-BglNH is an ideal candidate for further research and industrial applications.
DepartmentLMB
KeywordBeta-glucosidase Cellulase Salt-tolerant Glucose-enhanced Streptomyces Sp
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
Funding OrganizationThis study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104).
URL查看原文
Language英语
Funding OrganizationThis study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104). ; This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104).
WOS IDWOS:000316116500004
Citation statistics
Cited Times:18[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11060
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorzhsimd@scsio.ac.cn
Recommended Citation
GB/T 7714
[Mai, Zhimao,Yang, Jian,Tian, Xinpeng,et al. Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,169(5):1512-1522.
APA [Mai, Zhimao.,Yang, Jian.,Tian, Xinpeng.,Li, Jie.,Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China.,...&zhsimd@scsio.ac.cn.(2013).Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,169(5),1512-1522.
MLA [Mai, Zhimao,et al."Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 169.5(2013):1512-1522.
Files in This Item:
File Name/Size DocType Version Access License
Gene Cloning and Cha(550KB) 开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.