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学科主题: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
题名: Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete
作者: [Mai, Zhimao ; Yang, Jian ; Tian, Xinpeng ; Li, Jie ; Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China ; [Mai, Zhimao ; Yang, Jian ; Zhang, Si] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
通讯作者: zhsimd@scsio.ac.cn
关键词: beta-glucosidase ; Cellulase ; Salt-tolerant ; Glucose-enhanced ; Streptomyces sp
刊名: APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
发表日期: 2013
卷: 169, 期:5, 页:1512-1522
部门归属: LMB
资助者: This study was supported by the National Basic Research Program of China (No. 2010CB833801), the National High Technology Research and Development Program of China (863 Program, 2012AA092104).
摘要: The gene BglNH encoding a beta-glucosidase was cloned from a marine streptomycete. Sequence analysis revealed that BglNH encoded a 456-aa peptide with a calculated mass of 51 kDa. The deduced amino acid sequence of BglNH showed the highest identities of 61 % with known beta-glucosidases and contained a catalytic domain which belonged to the glycoside hydrolase family 1. The gene BglNH was expressed in Escherichia coli and the recombinant enzyme (r-BglNH) was purified. The optimum pH and temperature of r-BglNH were pH 6.0 and 45 A degrees C, respectively. The r-BglNH displayed the typical salt-tolerant and glucose-enhanced characteristics. Its activity was remarkably enhanced in the presence of 0.5 M NaCl (rose more than 1.6-fold) and 0.1 M glucose (rose more than 1.4-fold). Moreover, r-BglNH displayed good pH stability and metal tolerance. It remained stable after incubating with buffers from pH 4.0 to 10.0, and most metal ions had no significant inhibition on its activity. These properties indicate that r-BglNH is an ideal candidate for further research and industrial applications.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000316116500004
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11060
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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[Mai, Zhimao; Yang, Jian; Tian, Xinpeng; Li, Jie; Zhang, Si] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, RNAM Ctr Marine Microbiol, Guangdong Key Lab Marine Mat Med,South China Sea, Guangzhou 510301, Guangdong, Peoples R China; [Mai, Zhimao; Yang, Jian; Zhang, Si] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China.Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete,APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2013,169(5):1512-1522
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文件名: Gene Cloning and Characterization of a Novel Salt-Tolerant and Glucose-Enhanced beta-Glucosidase from a Marine Streptomycete.pdf
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