Gene cloning and function analysis of cytokine-induced suppressor of cytokine signaling (SOCS) from pearl oyster Pinctada fucata
[Liu, Wen-Guang; Huang, Xian-De; Wang, Qi; Zhao, Mi; Wu, Shan-Zeng; He, Mao-Xian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; hmx@scsio.ac.cn
2013
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
卷号34期号:3页码:849-854
摘要Cytokine-induced suppressor of cytokine signaling (SOCS) family acts as a negative regulator of cytokine receptor signaling to control excessive cytokine effects and inhibit a variety of signal transduction pathways, particularly the Janus kinases/signal transducers and activators of transcription (JAK/STAT) pathway. In present study, SOCS-2 homolog (PfSOCS-2) from pearl oyster Pinctada fucata was cloned and its gene has no intron. Multiple sequence alignments and phylogenetic analysis showed that PfSOCS-2 was clustered with other mollusk SOCS-2. LPS or polyI:C challenge and gene expression analysis revealed that PfSOCS-2 involved the innate immune response against bacterial and viral infections and that induction of PfSOCS-2 was varied with the different challenge stimulations. Furthermore, Dual-luciferase reporter assays showed that PfSOCS-2 involved in the regulation of vertebrate target genes containing the IFN-stimulated response element or NF-kappa B binding site in vitro. These results indicated that SOCS-2 from P. fucata plays a regulatory role against the stimulation. (c) 2013 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Pearl Oyster Pfsocs-2 Gene Cloning Immune Response
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助者The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145).
URL查看原文
语种英语
资助者The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145). ; The pearl oysters used in the study were reared at Marine Biology Research Station at Daya Bay, Chinese Academy of Sciences, Shenzhen, China. This study was supported by the National Science and technology program of China (2012AA10A410), the Funds of Knowledge Innovation program of Chinese Academy of Sciences (ZCX2-EW-Q21), and the National Natural Science Foundation of China (41176145).
WOS记录号WOS:000316523300013
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/11064
专题中科院海洋生物资源可持续利用重点实验室
通讯作者hmx@scsio.ac.cn
推荐引用方式
GB/T 7714
[Liu, Wen-Guang,Huang, Xian-De,Wang, Qi,et al. Gene cloning and function analysis of cytokine-induced suppressor of cytokine signaling (SOCS) from pearl oyster Pinctada fucata[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(3):849-854.
APA [Liu, Wen-Guang.,Huang, Xian-De.,Wang, Qi.,Zhao, Mi.,Wu, Shan-Zeng.,...&hmx@scsio.ac.cn.(2013).Gene cloning and function analysis of cytokine-induced suppressor of cytokine signaling (SOCS) from pearl oyster Pinctada fucata.FISH & SHELLFISH IMMUNOLOGY,34(3),849-854.
MLA [Liu, Wen-Guang,et al."Gene cloning and function analysis of cytokine-induced suppressor of cytokine signaling (SOCS) from pearl oyster Pinctada fucata".FISH & SHELLFISH IMMUNOLOGY 34.3(2013):849-854.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Gene cloning and fun(1047KB) 开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。