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Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)
[Shi, Yan; Zhu, Xinping; Chen, Kunci] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China; [Zhao, Zhe] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Zhang, Qiya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; zhuxinping_1964@yahoo.com.cn
2013
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume34Issue:1Pages:273-279
AbstractGRIM-19 is a nuclear encoded subunit of complex I that has been implicated in apoptosis. The protein participates in multiple functions including the innate immune response. GRIM-19 has been studied in humans and other mammals; however, fish GRIM-19 has not been well characterized. In this study, a new GRIM-19 gene, EcGRIM-19, was isolated from the orange-spotted grouper (Epinephelus coioides) cDNA library, which was constructed following LPS treatment. EcGRIM-19 is a 582-bp gene that encodes a 144-amino acid protein. The gene is a true ortholog of mammalian GRIM-19. EcGRIM-19 exhibits ubiquitous and constitutive expression in the different tissues of the orange-spotted grouper. The expression levels of EcGRIM-19 are altered in the gill, spleen, kidney and liver after induction with LPS. The subcellular localization analysis demonstrated that the EcGRIM-19 protein is localized predominantly in the mitochondria. In addition, amino acids 30-50 of the protein are responsible for the mitochondrial localization of EcGRIM-19. The caspase assay demonstrated that the overexpression of GRIM-19 enhanced the cellular sensitivity to interferon(IFN)-beta- and retinoic acid (RA)-induced death in HeLa cells. The data presented in this study are important for further understanding the EcGRIM-19 gene function in fish. (c) 2012 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordGrim-19 Epinephelus Coioides Tissue Distribution Subcellular Localization Overexpression
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationThe fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research. ; The fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research. ; The fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research. ; The fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research.
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Language英语
Funding OrganizationThe fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research. ; The fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research. ; The fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research. ; The fund from the Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences (LMB091006); the fund from the State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences (2010FB09); the fund from National Natural Science Foundation of China (41276163 and 30901117) and the Fundamental Research Funds from CAFS (2012A0403) supported this research.
WOS IDWOS:000314743600032
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11078
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorzhuxinping_1964@yahoo.com.cn
Recommended Citation
GB/T 7714
[Shi, Yan,Zhu, Xinping,Chen, Kunci] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China,et al. Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):273-279.
APA [Shi, Yan,Zhu, Xinping,Chen, Kunci] Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Minist Agr, Key Lab Trop & Subtrop Fishery Resource Applicat, Guangzhou 510380, Guangdong, Peoples R China,[Zhao, Zhe] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China,[Zhang, Qiya] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China,&zhuxinping_1964@yahoo.com.cn.(2013).Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides).FISH & SHELLFISH IMMUNOLOGY,34(1),273-279.
MLA [Shi, Yan,et al."Expression and functional characterization of a gene associated with retinoid-interferon-induced mortality 19 (GRIM-19) from orange-spotted grouper (Epinephelus coioides)".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):273-279.
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