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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Molecular cloning, characterization of one key molecule of teleost innate immunity from orange-spotted grouper (Epinephelus coioides): Serum amyloid A
作者: [Wei, Jingguang ; Guo, Minglan ; Qin, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China ; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
通讯作者: qinqw@scsio.ac.cn
关键词: Epinephelus coioides ; Singapore grouper iridovirus (SGIV) ; Serum amyloid A ; Molecular cloning ; Expression analysis
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2013
卷: 34, 期:1, 页:296-304
部门归属: LMB
资助者: This work was supported by grants from National High Technology Development Program of China (863)(2012AA092201), National Basic Research Program of China (973 program) (2012CB114402), the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-Q213, KZCX2-YW-BR-08), the National Natural Science Foundation of China (30930070, 31001124, 30725027, 31202022), and the Guangdong Province Natural Science Foundation (S2011040000953).
摘要: The orange-spotted grouper (Epinephelus coioides), a favorite marine food fish, is widely cultured in China and Southeast Asian countries. However, little is known about its acute phase response (APR) caused by viral diseases. Serum amyloid A (SAA) is a major acute phase protein (APP). In this study, a new SAA homologous (EcSAA) gene was cloned from grouper, E. coioides, by rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA sequence of SAA was 508 bp and contained a 363 bp open reading frame (ORF) coding for a protein of 121 aa. Similar to other fish known SAA genes, the EcSAA gene contained four exons and three introns. Quantitative real-time PCR analysis revealed that EcSAA mRNA is predominately expressed in liver and gill of grouper. Furthermore, the expression of EcSAA was differentially up-regulated in liver after infection with Staphyloccocus aureus, Vibrio vulnificus, Vibrio parahaemolyticus, Saccharomyces cerevisiae and Singapore grouper iridovirus (SGIV). Recombinant EcSAA (rEcSAA) was expressed in Escherichia BL21 (DE3) and purified for mouse anti-EcSAA serum preparation. The rEcSAA fusion protein was demonstrated to bind to all tested bacteria and yeast, and inhibit the replication of SGIV. Overexpression of EcSAA in grouper spleen (GS) cells could also inhibit the replication of SGIV. These results suggest that EcSAA may be an important molecule in the innate immunity of grouper. (c) 2012 Elsevier Ltd. All rights reserved.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000314743600035
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11079
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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[Wei, Jingguang; Guo, Minglan; Qin, Qiwei] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China.Molecular cloning, characterization of one key molecule of teleost innate immunity from orange-spotted grouper (Epinephelus coioides): Serum amyloid A,FISH & SHELLFISH IMMUNOLOGY,2013,34(1):296-304
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