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Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii
[Li, Jun; Chen, Jinhui; Zhang, Yang; Yu, Ziniu] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Lab Appl Marine Biol, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; carlzyu@scsio.ac.cn
2013
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume34Issue:1Pages:365-371
AbstractAllograft inflammatory factor-1 (AIF-1), an interferon (IFN)-gamma-inducible calcium-binding cytokine, is associated with the inflammatory response and defense. We cloned and analyzed the expression pattern of the AIF-1 gene of the pearl oyster Pinctada martensii, hereafter designated PmAIF-1. The full-length PmAIF-1 cDNA is 946 bp in length and consists of a 5'-untranslated region (UTR) of 120 bp, a 3'-UTR of 376 bp, and an open reading frame (ORF) of 450 bp encoding a polypeptide of 149 amino acids with an estimated molecular mass of 17 kDa. Sequence analysis reveals that PmAIF-1 contains two EF hand Ca+2-binding motifs like those in previously characterized AIF-1s while alignment with known AIF-1 protein sequences reveals higher similarity to invertebrate orthologs than to those of vertebrates. Quantitative PCR analysis reveals that PmAIF-1 is constitutively expressed, with the highest expression detected in hemocytes, and the expression level of PmAIF-1 mRNA was significantly up-regulated in hemocytes, gill, digestive gland under bacterial challenge and tissue injury. After challenged by gram-negative bacteria Vibrio alginolyticus and Vibrio parahaemolyticus, gram-positive bacteria Bacillus subtills, the expression level of this gene in hemocytes were all up-regulated and reached the maximum point at 12 h (5.80 folds, p < 0.01), 6 h (5.02 folds, P < 0.01) and 12 h (5.49 folds, P < 0.01), respectively. Under shell damage and mantle injury, PmAIF-1 mRNA increased gradually in the first 3 h and reached a peak of expression at 6 h post-injury. These findings suggest that PmAIF-1 is an acute-response protein involved in the innate immune responses of pearl oysters, and provide general information about the mechanisms of innate immune defense against bacterial infection in pearl oysters. (c) 2012 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordPinctada Martensii Allograft Inflammatory Factor-1 Hemocytes Immune Challenge Tissue Injury
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
Funding OrganizationThis work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review. ; This work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review. ; This work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review. ; This work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review.
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Language英语
Funding OrganizationThis work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review. ; This work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review. ; This work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review. ; This work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review.
WOS IDWOS:000314743600043
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/11080
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcarlzyu@scsio.ac.cn
Recommended Citation
GB/T 7714
[Li, Jun,Chen, Jinhui,Zhang, Yang,et al. Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(1):365-371.
APA [Li, Jun,Chen, Jinhui,Zhang, Yang,Yu, Ziniu] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Lab Appl Marine Biol, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China,[Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China,&carlzyu@scsio.ac.cn.(2013).Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii.FISH & SHELLFISH IMMUNOLOGY,34(1),365-371.
MLA [Li, Jun,et al."Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii".FISH & SHELLFISH IMMUNOLOGY 34.1(2013):365-371.
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