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学科主题: Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
题名: Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii
作者: [Li, Jun ; Chen, Jinhui ; Zhang, Yang ; Yu, Ziniu] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Lab Appl Marine Biol, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China ; [Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
通讯作者: carlzyu@scsio.ac.cn
关键词: Pinctada martensii ; Allograft inflammatory factor-1 ; Hemocytes ; Immune challenge ; Tissue injury
刊名: FISH & SHELLFISH IMMUNOLOGY
发表日期: 2013
卷: 34, 期:1, 页:365-371
部门归属: LMB
资助者: This work was supported by a National Basic Research Program of China (No. 2010CB126404), a National Science Foundation of China (No. 41176150) and the Knowledge Innovation Program of the Chinese Academy of Sciences (SQ200905). The authors thank Prof. Elizabeth De Stasio for her English review.
摘要: Allograft inflammatory factor-1 (AIF-1), an interferon (IFN)-gamma-inducible calcium-binding cytokine, is associated with the inflammatory response and defense. We cloned and analyzed the expression pattern of the AIF-1 gene of the pearl oyster Pinctada martensii, hereafter designated PmAIF-1. The full-length PmAIF-1 cDNA is 946 bp in length and consists of a 5'-untranslated region (UTR) of 120 bp, a 3'-UTR of 376 bp, and an open reading frame (ORF) of 450 bp encoding a polypeptide of 149 amino acids with an estimated molecular mass of 17 kDa. Sequence analysis reveals that PmAIF-1 contains two EF hand Ca+2-binding motifs like those in previously characterized AIF-1s while alignment with known AIF-1 protein sequences reveals higher similarity to invertebrate orthologs than to those of vertebrates. Quantitative PCR analysis reveals that PmAIF-1 is constitutively expressed, with the highest expression detected in hemocytes, and the expression level of PmAIF-1 mRNA was significantly up-regulated in hemocytes, gill, digestive gland under bacterial challenge and tissue injury. After challenged by gram-negative bacteria Vibrio alginolyticus and Vibrio parahaemolyticus, gram-positive bacteria Bacillus subtills, the expression level of this gene in hemocytes were all up-regulated and reached the maximum point at 12 h (5.80 folds, p < 0.01), 6 h (5.02 folds, P < 0.01) and 12 h (5.49 folds, P < 0.01), respectively. Under shell damage and mantle injury, PmAIF-1 mRNA increased gradually in the first 3 h and reached a peak of expression at 6 h post-injury. These findings suggest that PmAIF-1 is an acute-response protein involved in the innate immune responses of pearl oysters, and provide general information about the mechanisms of innate immune defense against bacterial infection in pearl oysters. (c) 2012 Elsevier Ltd. All rights reserved.
语种: 英语
原文出处: 查看原文
WOS记录号: WOS:000314743600043
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内容类型: 期刊论文
URI标识: http://ir.scsio.ac.cn/handle/344004/11080
Appears in Collections:中科院海洋生物资源可持续利用重点实验室_期刊论文

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Recommended Citation:
[Li, Jun; Chen, Jinhui; Zhang, Yang; Yu, Ziniu] Chinese Acad Sci, Key Lab Marine Bioresources Sustainable Utilizat, Lab Appl Marine Biol, S China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China; [Li, Jun] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China.Expression of allograft inflammatory factor-1 (AIF-1) in response to bacterial challenge and tissue injury in the pearl oyster, Pinctada martensii,FISH & SHELLFISH IMMUNOLOGY,2013,34(1):365-371
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