Genomic insights into the taxonomic status of the Bacillus cereus group
Liu, Yang1; Lai, Qiliang1; Goeker, Markus1; Meier-Kolthoff, Jan P.1; Wang, Meng1; Sun, Yamin1; Wang, Lei1; Shao, Zongze1; shaozz@163.com
2015
发表期刊SCIENTIFIC REPORTS
卷号5页码:14082-
摘要The identification and phylogenetic relationships of bacteria within the Bacillus cereus group are controversial. This study aimed at determining the taxonomic affiliations of these strains using the whole-genome sequence-based Genome BLAST Distance Phylogeny (GBDP) approach. The GBDP analysis clearly separated 224 strains into 30 clusters, representing eleven known, partially merged species and accordingly 19-20 putative novel species. Additionally, 16S rRNA gene analysis, a novel variant of multi-locus sequence analysis (nMLSA) and screening of virulence genes were performed. The 16S rRNA gene sequence was not sufficient to differentiate the bacteria within this group due to its high conservation. The nMLSA results were consistent with GBDP. Moreover, a fast typing method was proposed using the pycA gene, and where necessary, the ccpA gene. The pXO plasmids and cry genes were widely distributed, suggesting little correlation with the phylogenetic positions of the host bacteria. This might explain why classifications based on virulence characteristics proved unsatisfactory in the past. In summary, this is the first large-scale and systematic study of the taxonomic status of the bacteria within the B. cereus group using whole-genome sequences, and is likely to contribute to further insights into their pathogenicity, phylogeny and adaptation to diverse environments.
部门归属LMB
学科领域Science & Technology - Other Topics
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14772
专题中科院海洋生物资源可持续利用重点实验室
通讯作者shaozz@163.com
作者单位1.[Liu, Yang
2.Lai, Qiliang
3.Shao, Zongze] State Key Lab Breeding Base Marine Genet Resource, Xiamen 361005, Peoples R China
4.[Liu, Yang
5.Lai, Qiliang
6.Shao, Zongze] SOA, Ins Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China
7.[Liu, Yang
8.Lai, Qiliang
9.Shao, Zongze] South China Sea Bio Resource Exploitat & Utilizat, Xiamen 361005, Peoples R China
10.[Liu, Yang
11.Lai, Qiliang
12.Shao, Zongze] Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Xiamen 361005, Peoples R China
13.[Liu, Yang
14.Lai, Qiliang
15.Shao, Zongze] Key Lab Marine Genet Resources Fujian Prov, Xiamen 361005, Peoples R China
16.[Goeker, Markus
17.Meier-Kolthoff, Jan P.] Leibniz Inst DSMZ German Collect Microorganisms, D-38124 Braunschweig, Germany
18.[Goeker, Markus
19.Meier-Kolthoff, Jan P.] Cell Cultures GmbH, D-38124 Braunschweig, Germany
20.[Wang, Meng
21.Sun, Yamin
22.Wang, Lei] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, Tianjin 300071, Peoples R China
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GB/T 7714
Liu, Yang,Lai, Qiliang,Goeker, Markus,et al. Genomic insights into the taxonomic status of the Bacillus cereus group[J]. SCIENTIFIC REPORTS,2015,5:14082-.
APA Liu, Yang.,Lai, Qiliang.,Goeker, Markus.,Meier-Kolthoff, Jan P..,Wang, Meng.,...&shaozz@163.com.(2015).Genomic insights into the taxonomic status of the Bacillus cereus group.SCIENTIFIC REPORTS,5,14082-.
MLA Liu, Yang,et al."Genomic insights into the taxonomic status of the Bacillus cereus group".SCIENTIFIC REPORTS 5(2015):14082-.
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