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Genomic insights into the taxonomic status of the Bacillus cereus group
Liu, Yang1; Lai, Qiliang1; Goeker, Markus1; Meier-Kolthoff, Jan P.1; Wang, Meng1; Sun, Yamin1; Wang, Lei1; Shao, Zongze1;
AbstractThe identification and phylogenetic relationships of bacteria within the Bacillus cereus group are controversial. This study aimed at determining the taxonomic affiliations of these strains using the whole-genome sequence-based Genome BLAST Distance Phylogeny (GBDP) approach. The GBDP analysis clearly separated 224 strains into 30 clusters, representing eleven known, partially merged species and accordingly 19-20 putative novel species. Additionally, 16S rRNA gene analysis, a novel variant of multi-locus sequence analysis (nMLSA) and screening of virulence genes were performed. The 16S rRNA gene sequence was not sufficient to differentiate the bacteria within this group due to its high conservation. The nMLSA results were consistent with GBDP. Moreover, a fast typing method was proposed using the pycA gene, and where necessary, the ccpA gene. The pXO plasmids and cry genes were widely distributed, suggesting little correlation with the phylogenetic positions of the host bacteria. This might explain why classifications based on virulence characteristics proved unsatisfactory in the past. In summary, this is the first large-scale and systematic study of the taxonomic status of the bacteria within the B. cereus group using whole-genome sequences, and is likely to contribute to further insights into their pathogenicity, phylogeny and adaptation to diverse environments.
Subject AreaScience & Technology - Other Topics
Document Type期刊论文
Affiliation1.[Liu, Yang
2.Lai, Qiliang
3.Shao, Zongze] State Key Lab Breeding Base Marine Genet Resource, Xiamen 361005, Peoples R China
4.[Liu, Yang
5.Lai, Qiliang
6.Shao, Zongze] SOA, Ins Oceanog 3, Key Lab Marine Genet Resources, Xiamen 361005, Peoples R China
7.[Liu, Yang
8.Lai, Qiliang
9.Shao, Zongze] South China Sea Bio Resource Exploitat & Utilizat, Xiamen 361005, Peoples R China
10.[Liu, Yang
11.Lai, Qiliang
12.Shao, Zongze] Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Xiamen 361005, Peoples R China
13.[Liu, Yang
14.Lai, Qiliang
15.Shao, Zongze] Key Lab Marine Genet Resources Fujian Prov, Xiamen 361005, Peoples R China
16.[Goeker, Markus
17.Meier-Kolthoff, Jan P.] Leibniz Inst DSMZ German Collect Microorganisms, D-38124 Braunschweig, Germany
18.[Goeker, Markus
19.Meier-Kolthoff, Jan P.] Cell Cultures GmbH, D-38124 Braunschweig, Germany
20.[Wang, Meng
21.Sun, Yamin
22.Wang, Lei] Nankai Univ, TEDA Sch Biol Sci & Biotechnol, Tianjin 300071, Peoples R China
Recommended Citation
GB/T 7714
Liu, Yang,Lai, Qiliang,Goeker, Markus,et al. Genomic insights into the taxonomic status of the Bacillus cereus group[J]. SCIENTIFIC REPORTS,2015,5:14082-.
APA Liu, Yang.,Lai, Qiliang.,Goeker, Markus.,Meier-Kolthoff, Jan P..,Wang, Meng.,...& insights into the taxonomic status of the Bacillus cereus group.SCIENTIFIC REPORTS,5,14082-.
MLA Liu, Yang,et al."Genomic insights into the taxonomic status of the Bacillus cereus group".SCIENTIFIC REPORTS 5(2015):14082-.
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