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Halovulum dunhuangense gen. nov., sp nov., isolated from a saline terrestrial spring
Sun, Fengqin1; Du, Yaping1; Liu, Xiupian1; Lai, Qiliang1; Shao, Zongze1;
Abstractbacterial strain, YY0-30(T), isolated from a mixed water sand sediment sample collected from a terrestrial spring located in Dunhuang, China, was characterized with respect to its morphology, physiology and taxonomy. Cells of the strain were Gram-stain-negative, aerobic, oxidase- and catalase-positive, non-flagellated, oval to rod-shaped (0.5-1.0 gm wide and 1.1-6.6 mu m long) and divided by binary fission. Growth was observed in the presence of 0-10.0% (w/v) NaCl with optimal growth at 0-3.0%, at pH 6.0-9.0 (optimum pH 7.0-8.5) and at 10-45 degrees C (optimum 30-37 degrees C). The isolate could reduce nitrate to nitrite and hydrolyse aesculin and gelatin (weakly), but was unable to degrade Tween 80 or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YYQ-30(T) belongs to the family Rhodobacteraceae and forms a distinct lineage with the type strain of Albimonas donghaensis and forms a branch within a cluster constituted by the type strains of species of the genera Albimonas, Rhodovulum, Albidovulum, Haematobacter and Tropicimonas; levels of 16S rRNA gene sequence similarity between strain YY0-30T and members of related genera ranged from 94.1 to 89.7 %. Strain YYQ-30(T) contained Q-10 as the predominant ubiquinone and summed feature 8 (C-18:1 omega 7c and/or C-18 : 1 omega 6c; 70.0 %), C-18 : 0 (9.5 CYO, summed feature 2 (one or more of C-14 : 0 3-OH, iso-C-16 :1 I and C-12 : 0 aldehyde; 6.9 %) and 11-methyl C-18 : 1 omega 7c (6.0 %) as the principal fatty acids. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, two unidentified aminolipids and five unknown lipids. The pufLM gene was detected. The G+C content of the genomic DNA was 71.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain YYQ-30(T) is considered to represent a novel species in a new genus within the family Rhodobacteraceae, for which the name Halovulum dunhuangense gen. nov., sp. nov. is proposed. The type strain of Halovulum dunhuangense is YYQ-30(T) (=LMG 27418(T)=MCCC 1A06483(T)).
Subject AreaMicrobiology
Document Type期刊论文
Affiliation1.[Shao, Zongze] SOA, South China Sea Bioresource Exploitat & Utilizat, State Key Lab Breeding Base Marine Genet Resource, Key Lab Marine Biogenet Resources,Inst Oceanog 3, Xiamen 361005, Peoples R China
2.Key Lab Marine Genet Resources Fujian Prov, Xiamen 361005, Peoples R China
Recommended Citation
GB/T 7714
Sun, Fengqin,Du, Yaping,Liu, Xiupian,et al. Halovulum dunhuangense gen. nov., sp nov., isolated from a saline terrestrial spring[J]. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,2015,65:2810-2816.
APA Sun, Fengqin,Du, Yaping,Liu, Xiupian,Lai, Qiliang,Shao, Zongze,& dunhuangense gen. nov., sp nov., isolated from a saline terrestrial spring.INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,65,2810-2816.
MLA Sun, Fengqin,et al."Halovulum dunhuangense gen. nov., sp nov., isolated from a saline terrestrial spring".INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 65(2015):2810-2816.
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