Identification and characterization of a HEPN-MNT family type II toxin-antitoxin in Shewanella oneidensis
Yao, Jianyun1; Guo, Yunxue1; Zeng, Zhenshun1; Liu, Xiaoxiao1; Shi, Fei1; Wang, Xiaoxue1; xxwang@scsio.ac.cn
2015
发表期刊MICROBIAL BIOTECHNOLOGY
卷号8期号:6页码:961-973
摘要Toxin-antitoxin (TA) systems are prevalent in bacteria and archaea. However, related studies in the ecologically and bioelectrochemically important strain Shewanella oneidensis are limited. Here, we show that SO_3166, a member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) superfamily, strongly inhibited cell growth in S. oneidensis and Escherichia coli. SO_3165, a putative minimal nucleotidyltransferase (MNT), neutralized the toxicity of SO_3166. Gene SO_3165 lies upstream of SO_3166, and they are co-transcribed. Moreover, the SO_3165 and SO_3166 proteins interact with each other directly in vivo, and antitoxin SO_3165 bound to the promoter of the TA operon and repressed its activity. Finally, the conserved Rx4-6H domain in HEPN family was identified in SO_3166. Mutating either the R or H abolished SO_3166 toxicity, confirming that Rx4-6H domain is critical for SO_3166 activity. Taken together, these results demonstrate that SO_3166 and SO_3165 in S. oneidensis form a typical type II TA pair. This TA pair plays a critical role in regulating bacterial functions because its disruption led to impaired cell motility in S. oneidensis. Thus, we demonstrated for the first time that HEPN-MNT can function as a TA system, thereby providing important insights into the understanding of the function and regulation of HEPNs and MNTs in prokaryotes.
部门归属LMB
学科领域Biotechnology & Applied Microbiology ; Microbiology
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14778
专题中科院海洋生物资源可持续利用重点实验室
通讯作者xxwang@scsio.ac.cn
作者单位1.[Yao, Jianyun
2.Guo, Yunxue
3.Zeng, Zhenshun
4.Liu, Xiaoxiao
5.Shi, Fei
6.Wang, Xiaoxue] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China
7.[Yao, Jianyun
8.Zeng, Zhenshun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Yao, Jianyun,Guo, Yunxue,Zeng, Zhenshun,et al. Identification and characterization of a HEPN-MNT family type II toxin-antitoxin in Shewanella oneidensis[J]. MICROBIAL BIOTECHNOLOGY,2015,8(6):961-973.
APA Yao, Jianyun.,Guo, Yunxue.,Zeng, Zhenshun.,Liu, Xiaoxiao.,Shi, Fei.,...&xxwang@scsio.ac.cn.(2015).Identification and characterization of a HEPN-MNT family type II toxin-antitoxin in Shewanella oneidensis.MICROBIAL BIOTECHNOLOGY,8(6),961-973.
MLA Yao, Jianyun,et al."Identification and characterization of a HEPN-MNT family type II toxin-antitoxin in Shewanella oneidensis".MICROBIAL BIOTECHNOLOGY 8.6(2015):961-973.
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