Characterization of a thermostable and alkali-stable alpha-amylase from deep-sea bacterium Flammeovirga pacifica
Zhou, Guilan1; Jin, Min1; Cai, Yaping1; Zeng, Runying1; zeng@tio.org.cn
2015
发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷号80页码:676-682
摘要A thermostable a-amylase (designated as Amy16) has been previously identified in Flammeovirga pacifica isolated from deep-sea sediments. The DNA sequence of Amy16 exhibited no significant similarity with those of any known protein, including the glycoside hydrolases. Amino acid sequence analysis revealed that Amy16 belonged to GH13 family and possessed a conserved DXEXD motif, which was essential for its hydrolysis activities. The recombinant Amyl 6 purified with Ni+ affinity column after its heterologous expression in Escherichia coli cells was most active at 50 degrees C and retained more than 81% of its initial activity after incubation at 60 degrees C for 20 min. The optimal pH for Amy16 was determined to be 6.5, and a good tolerance to alkaline environment was observed. Low concentration of Mg2+, Sr2+, Na+ and K+ slightly increased the activity of Amy16. Results of thin layer chromatography experiments revealed that Amyl 6 was able to hydrolyse starch into maltose in a time-dependent manner, suggesting that Amyl 6 is a liquid-type endoenzyme with starch hydrolysis activities. Therefore, our study presented thermostable and alkali-stable Amyl 6, which may be suitable for use as an additive in detergents. (C) 2015 Elsevier B.V. All rights reserved.
部门归属LMB
关键词Alpha-amylase Thermostable Alkali-stable Deep-sea Flammeovirga Pacifica
学科领域Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14780
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zeng@tio.org.cn
作者单位1.[Zhou, Guilan
2.Jin, Min
3.Zeng, Runying] SOA, State Key Lab Breeding Base Marine Genet Resource, Inst Oceanog 3, Xiamen 361005, Peoples R China
4.[Cai, Yaping] Xiamen Entry Exit Inspect & Quarantine Bur IQTC, Inspect & Quarantine Technol Ctr, Xiamen 361100, Peoples R China
5.[Zeng, Runying] South China Sea Bioresource Exploitat & Utilizat, Guangzhou 510000, Guangdong, Peoples R China
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Zhou, Guilan,Jin, Min,Cai, Yaping,et al. Characterization of a thermostable and alkali-stable alpha-amylase from deep-sea bacterium Flammeovirga pacifica[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2015,80:676-682.
APA Zhou, Guilan,Jin, Min,Cai, Yaping,Zeng, Runying,&zeng@tio.org.cn.(2015).Characterization of a thermostable and alkali-stable alpha-amylase from deep-sea bacterium Flammeovirga pacifica.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,80,676-682.
MLA Zhou, Guilan,et al."Characterization of a thermostable and alkali-stable alpha-amylase from deep-sea bacterium Flammeovirga pacifica".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 80(2015):676-682.
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