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Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge
Cheng, Chang-Hong1; Yang, Fang-Fang1; Liao, Shao-An1; Miao, Yu-Tao1; Ye, Chao-Xia1; Wang, An-Li1; Liu, Jin-Chang1; Liu, Li-Wei1; chengchanghong09@163.com; chaoxia_ye@yahoo.com.cn; wanganl@scnu.edu.cn
2015
Source PublicationFISH PHYSIOLOGY AND BIOCHEMISTRY
Volume41Issue:4Pages:1053-1064
AbstractApoptosis plays a crucial role in many biological processes, including development, cellular homeostasis and immune responses. The BCL-2 family is a key regulator of the mitochondrial response to apoptotic signals in the intrinsic pathway. In this study, we identified and characterized the cDNA and expression pattern of pufferfish BCL-2 (PfBCL-2). The full-length cDNA of PfBCL-2 was 1412 bp with an open reading frame of 657 bp encoding a putative protein of 219 amino acids (Accession no: KP898414). The calculated molecular mass of the PfBCL-2 was 24.2 kDa with a predicted isoelectric point of 5.27. The deduced PfBCL-2 protein exhibited four highly conserved BCL-2 homology domains, suggesting that PfBCL-2 may play a similar role in the apoptotic-signaling pathway as in other species. Real-time PCR results showed that PfBCL-2 transcript was expressed in a wide range of tissues but exhibited the greatest level of expression in blood. Transcriptional responses of PfBCL-2 exhibited different spatial and temporal expression profiles in liver and blood after bacterial infection. PfBcl-2 transcript was significantly up-regulated in liver at 6, 12, 24 and 48 h (with maximum induction at 48 h) and was up-regulated in blood at 3, 6, 12 and 24 h (with maximum induction at 12 h). Meanwhile, recombinant PfBCL-2 fused with His(6) tag was efficiently expressed in Escherichia coli BL21 (DE3) and purified using Ni-nitrilotriacetic acid resin. Western blot analysis indicated that its protein level appeared to be elevated during the initial bacterial infection. These results suggest that PfBCL-2 plays important roles in immune responses against bacteria challenge.
DepartmentLMB
KeywordTakifugu Obscurus Apoptosis Bcl-2 Immune Response Gene Expression
Subject AreaBiochemistry & Molecular Biology ; Fisheries ; Physiology
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14798
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorchengchanghong09@163.com; chaoxia_ye@yahoo.com.cn; wanganl@scnu.edu.cn
Affiliation1.[Cheng, Chang-Hong
2.Liao, Shao-An
3.Miao, Yu-Tao
4.Ye, Chao-Xia
5.Wang, An-Li
6.Liu, Jin-Chang
7.Liu, Li-Wei] S China Normal Univ, Guangdong Higher Educ Inst, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Key Lab Ecol & Environm Sci,Sch Life Sci, Guangzhou 510631, Guangdong, Peoples R China
8.[Yang, Fang-Fang] Chinese Acad Sci, S China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Cheng, Chang-Hong,Yang, Fang-Fang,Liao, Shao-An,et al. Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2015,41(4):1053-1064.
APA Cheng, Chang-Hong.,Yang, Fang-Fang.,Liao, Shao-An.,Miao, Yu-Tao.,Ye, Chao-Xia.,...&wanganl@scnu.edu.cn.(2015).Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge.FISH PHYSIOLOGY AND BIOCHEMISTRY,41(4),1053-1064.
MLA Cheng, Chang-Hong,et al."Identification, characterization and functional analysis of anti-apoptotic protein BCL-2-like gene from pufferfish, Takifugu obscurus, responding to bacterial challenge".FISH PHYSIOLOGY AND BIOCHEMISTRY 41.4(2015):1053-1064.
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