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Real-time Imaging of Rabies Virus Entry into Living Vero cells
Xu, Haijiao1; Hao, Xian1; Wang, Shaowen1; Wang, Zhiyong1; Cai, Mingjun1; Jiang, Junguang1; Qin, Qiwei1; Zhang, Maolin1; Wang, Hongda1;;
AbstractUnderstanding the mechanism of rabies virus (RABV) infection is vital for prevention and therapy of virulent rabies. However, the infection mechanism remains largely uncharacterized due to the limited methods and viral models. Herein, we utilized a powerful single-virus tracking technique to dynamically and globally visualize the infection process of the live attenuated rabies vaccine strain-SRV9 in living Vero cells. Firstly, it was found that the actin-enriched filopodia is in favor of virus reaching to the cell body. Furthermore, by carrying out drug perturbation experiments, we confirmed that RABV internalization into Vero cells proceeds via classical dynamin-dependent clathrin-mediated endocytosis with requirement for intact actin, but caveolae-dependent endocytosis is not involved. Then, our real-time imaging results unambiguously uncover the characteristics of viral internalization and cellular transport dynamics. In addition, our results directly and quantitatively reveal that the intracellular motility of internalized RABV particles is largely microtubule-dependent. Collectively, our work is crucial for understanding the initial steps of RABV infection, and elucidating the mechanisms of post-infection. Significantly, the results provide profound insight into development of novel and effective antiviral targets.
Subject AreaScience & Technology - Other Topics
Document Type期刊论文
Affiliation1.[Xu, Haijiao
2.Hao, Xian
3.Wang, Zhiyong
4.Cai, Mingjun
5.Jiang, Junguang
6.Wang, Hongda] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
7.[Wang, Shaowen
8.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou, Guangdong, Peoples R China
9.[Wang, Shaowen
10.Qin, Qiwei
11.Wang, Hongda] Univ Chinese Acad Sci, Beijing, Peoples R China
12.[Zhang, Maolin] Jilin Univ, Minist Educ, Inst Zoonosis, Key Lab Zoonosis, Changchun 130023, Jilin, Peoples R China
Recommended Citation
GB/T 7714
Xu, Haijiao,Hao, Xian,Wang, Shaowen,et al. Real-time Imaging of Rabies Virus Entry into Living Vero cells[J]. SCIENTIFIC REPORTS,2015,5:11753-.
APA Xu, Haijiao.,Hao, Xian.,Wang, Shaowen.,Wang, Zhiyong.,Cai, Mingjun.,...& Imaging of Rabies Virus Entry into Living Vero cells.SCIENTIFIC REPORTS,5,11753-.
MLA Xu, Haijiao,et al."Real-time Imaging of Rabies Virus Entry into Living Vero cells".SCIENTIFIC REPORTS 5(2015):11753-.
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