Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda
Ge, Qianqian1; Liang, Junping1; Li, Jitao1; Li, Jian1; Duan, Yafei1; Zhao, Fazhen1; Ren, Hai1; ge2682@126.com; lijian@ysfri.ac.cn
2015
发表期刊FISHERIES SCIENCE
卷号81期号:4页码:699-711
摘要Rel/NF-kappa B transcription factors play critical roles in induction and regulation of innate immune response in organisms. In this study, the full length of a Relish homolog cDNA from Exopalaemon carinicauda named EcRelish was 2141 bp encoding a 660 amino-acid polypeptide. EcRelish cDNA contained a conserved Rel homology domain and two nucleus localization signals. Sequence analysis indicated that the deduced amino acid sequence of the EcRelish showed high similarities to that of other crustaceans. Real time RT-PCR analysis showed that EcRelish mRNA expressed with different levels in tested tissues, and the highest expression was observed in the hemocytes. With longer infection time, the cumulative mortality rates increased gradually followed by the proliferation of Vibrio anguillarum and WSSV. The expression profiles of EcRelish gene were analyzed after V. a nguillarum, white spot syndrome virus (WSSV) challenge, and ammonia-N stress. The results showed that the expression levels of EcRelish mRNA in the hemocytes were up-regulated at 1-24 h after V. a nguillarum challenge. Meanwhile, the expression levels of EcRelish mRNA were up-regulated at 3 h after WSSV challenge. The expression of EcRelish in hemocytes was down-regulated significantly under ammonia-N stress during the experimental time. The results indicated that EcRelish might be involved in immune defense against pathogens and ammonia-N stress in E. c arinicauda.
部门归属LMB
关键词Exopalaemon Carinicauda Relish Cloning Vibrio Anguillarum Wssv Ammonia-n Stress Expression
学科领域Fisheries
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14809
专题中科院海洋生物资源可持续利用重点实验室
通讯作者ge2682@126.com; lijian@ysfri.ac.cn
作者单位1.[Ge, Qianqian] Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China
2.[Ge, Qianqian
3.Li, Jitao
4.Li, Jian
5.Zhao, Fazhen
6.Ren, Hai] Chinese Acad Fishery Sci, Key Lab Sustainable Utilizat Marine Fisheries Res, Minist Agr, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
7.[Liang, Junping] Henan Normal Univ, Coll Fisheries, Xinxiang 453007, Peoples R China
8.[Duan, Yafei] Chinese Acad Fishery Sci, Key Lab South China Sea Fishery Resources Exploit, Minist Agr, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
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Ge, Qianqian,Liang, Junping,Li, Jitao,et al. Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda[J]. FISHERIES SCIENCE,2015,81(4):699-711.
APA Ge, Qianqian.,Liang, Junping.,Li, Jitao.,Li, Jian.,Duan, Yafei.,...&lijian@ysfri.ac.cn.(2015).Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda.FISHERIES SCIENCE,81(4),699-711.
MLA Ge, Qianqian,et al."Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda".FISHERIES SCIENCE 81.4(2015):699-711.
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