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Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, Danio rerio
Qi, Lin1; Xiang, Zhiming1; zhimingxiang@scsio.ac.cn
2015
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume45Issue:1Pages:59-66
AbstractThe death domain-associated protein Daxx exerts many functions including the induction and inhibition of apoptosis, regulation of chromatin remodeling and gene transcription. In this report, we have cloned and characterized a Daxx ortholog from the zebrafish, Danio rerio. The bioinformatics analysis results indicated that the open reading frame (ORF) of zebrafish Daxx is 2,151bp long and encodes a putative protein of 716 amino acids containing Daxx domain. Though quantitative PCR analyses, Daxx mRNA was detected in embryonic development from 6 h to 120 h and in all 11 selected zebrafish tissues, and the expression of Daxx was increased first and then decreased during megalocytivirus infectious spleen and kidney necrosis virus (ISKNV) infection. Fluorescence microscopy indicated that the full-length protein was located in the nuclei of the tested Hela cells uniformly but punctiform distribution in HEK293T. In the luciferase report assays, the GAL4-Daxx fusion protein inhibited the transcriptional activity of L8G5-Luc reporter gene showed that Daxx might act as a transcriptional repressor, following the overexpression in HEK293T, the activation of NF-kappa B-Luc and p53/p21-Luc reporter genes were repressed by the protein. These results suggested that Daxx might play definite role in apoptosis and innate immunity in zebrafish. (C) 2015 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordZebrafish Apoptosis Innate Immunity Isknv Daxx
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14811
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorzhimingxiang@scsio.ac.cn
Affiliation1.[Qi, Lin] Railway Police Coll, Zhengzhou 450053, Peoples R China
2.[Qi, Lin
3.Xiang, Zhiming] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Qi, Lin,Xiang, Zhiming,zhimingxiang@scsio.ac.cn. Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, Danio rerio[J]. FISH & SHELLFISH IMMUNOLOGY,2015,45(1):59-66.
APA Qi, Lin,Xiang, Zhiming,&zhimingxiang@scsio.ac.cn.(2015).Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, Danio rerio.FISH & SHELLFISH IMMUNOLOGY,45(1),59-66.
MLA Qi, Lin,et al."Molecular cloning and expression analysis of an apoptosis-associated gene Daxx from zebrafish, Danio rerio".FISH & SHELLFISH IMMUNOLOGY 45.1(2015):59-66.
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