Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane
Pan, Yangang1; Wang, Shaowen1; Shan, Yuping1; Zhang, Dinglin1; Gao, Jing1; Zhang, Min1; Liu, Shuheng1; Cai, Mingjun1; Xu, Haijiao1; Li, Guohui1; Qin, Qiwei1; Wang, Hongda1; ghli@dicp.ac.cn; qinqw@scsio.ac.cn; hdwang@ciac.ac.cn
2015
发表期刊SMALL
卷号11期号:23页码:2782-2788
摘要The first step in most viral infections is the penetration of the cell membrane via endocytosis. However, the underlying mechanism of this important process has not been quantitatively characterized; for example, the velocity and force of a single virion during invagination remain unknown. Here, the endocytosis of a single live virion (Singapore grouper iridovirus, SGIV) through the apical membranes of a host cell is monitored by developing and using a novel ultrafast (at the microsecond level) tracking technique: force tracing. For the first time, these results unambiguously reveal that the maximum velocity during the cell entry of a single SGIV by membrane invagination is approximately 200 nm s(-1), the endocytic force is approximately 60.8 +/- 18.5 pN, and the binding energy density increases with the engulfment depth. This report utilizing high temporospatial resolution (subnanometer and microsecond levels) approaches provides new insight into the dynamic process of viral infection via endocytosis and the mechanism of membrane invagination at the single-particle level.
部门归属LMB
学科领域Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
URL查看原文
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14813
专题中科院海洋生物资源可持续利用重点实验室
通讯作者ghli@dicp.ac.cn; qinqw@scsio.ac.cn; hdwang@ciac.ac.cn
作者单位1.[Pan, Yangang
2.Shan, Yuping
3.Gao, Jing
4.Zhang, Min
5.Liu, Shuheng
6.Cai, Mingjun
7.Xu, Haijiao
8.Wang, Hongda] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Electroanalyt Chem, Changchun 130022, Jilin, Peoples R China
9.[Pan, Yangang
10.Wang, Shaowen
11.Gao, Jing
12.Zhang, Min
13.Wang, Hongda] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
14.[Wang, Shaowen
15.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
16.[Shan, Yuping] Univ S Carolina, Dept Chem & Biochem, Columbia, SC 29208 USA
17.[Zhang, Dinglin
18.Li, Guohui] Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Liaoning, Peoples R China
19.[Shan, Yuping] Sch Chem & Life Sci, Adv Inst Mat Sci, Changchun 130012, Peoples R China
推荐引用方式
GB/T 7714
Pan, Yangang,Wang, Shaowen,Shan, Yuping,et al. Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane[J]. SMALL,2015,11(23):2782-2788.
APA Pan, Yangang.,Wang, Shaowen.,Shan, Yuping.,Zhang, Dinglin.,Gao, Jing.,...&hdwang@ciac.ac.cn.(2015).Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane.SMALL,11(23),2782-2788.
MLA Pan, Yangang,et al."Ultrafast Tracking of a Single Live Virion During the Invagination of a Cell Membrane".SMALL 11.23(2015):2782-2788.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Ultrafast Tracking o(812KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。