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Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)
Luo, Xing1; Chen, Ting1; Zhong, Ming1; Jiang, Xiao1; Zhang, Lvping1; Ren, Chunhua1; Hu, Chaoqun1; luoxingzky@163.com; chan1010@scsio.ac.cn; michell.mzhong@hotmail.com; jiangxiao4367@126.com; lpzhang@scsio.ac.cn; rosemary166@sina.com; cqhu@scsio.ac.cn
2015
Source PublicationPEPTIDES
Volume68Pages:58-63
AbstractMolt-inhibiting hormone (MIH), a peptide member of the crustacean hyperglycemic hormone (CHH) family, is commonly considered as a negative regulator during the molt cycle in crustaceans. Phylogenetic analysis of CHH family peptides in penaeidae shrimps suggested that there is no significant differentiation between MIH and vitellogenesis-inhibiting hormone (VIH, another peptide member of CHH family), by far the most potent negative regulator of crustacean vitellogenesis known. Thus, MIH may also play a role in regulating vitellogenesis. In this study, two previously reported putative MIHs (LivMIH1 and LivMIH2) in the Pacific white shrimp (Litopenaeus vannamei) were expressed in Escherichia coli, purified by immobilized metal ion affinity chromatography (IMAC) and further confirmed by western blot. Regulation of vitellogenin (VTG) mRNA expression by recombinant LivMIH1 and LivMIH2 challenge was performed by both in vitro hepatopancreatic primary cells culture and in vivo injection approaches. In in vitro primary culture of shrimp hepatopancreatic cells, only LivMIH2 but not LivMIH1 administration could improve the mRNA expression of VTG. In in vivo injection experiments, similarly, only LivMIH2 but not LivMIH1 could stimulate hepatopancreatic VTG gene expression and induce ovary maturation. Our study may provide evidence for one isoform of MIH (MIH2 in L. vannamei) may serve as one of the mediators of the physiological progress of molting and vitellogenesis. Our study may also give new insight in CHH family peptides regulating reproduction in crustaceans, in particular penaeidae shrimps. (C) 2014 Elsevier Inc. All rights reserved.
DepartmentLMB
KeywordMih Vih Litopenaeus Vannamei Vitellogenesis Reproduction Hepatopancreas
Subject AreaBiochemistry & Molecular Biology ; Pharmacology & Pharmacy
URL查看原文
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14828
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorluoxingzky@163.com; chan1010@scsio.ac.cn; michell.mzhong@hotmail.com; jiangxiao4367@126.com; lpzhang@scsio.ac.cn; rosemary166@sina.com; cqhu@scsio.ac.cn
Affiliation1.[Luo, Xing
2.Chen, Ting
3.Zhong, Ming
4.Jiang, Xiao
5.Zhang, Lvping
6.Ren, Chunhua
7.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou, Guangdong, Peoples R China
8.[Luo, Xing
9.Zhong, Ming] Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Luo, Xing,Chen, Ting,Zhong, Ming,et al. Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)[J]. PEPTIDES,2015,68:58-63.
APA Luo, Xing.,Chen, Ting.,Zhong, Ming.,Jiang, Xiao.,Zhang, Lvping.,...&cqhu@scsio.ac.cn.(2015).Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei).PEPTIDES,68,58-63.
MLA Luo, Xing,et al."Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)".PEPTIDES 68(2015):58-63.
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