Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)
Luo, Xing1; Chen, Ting1; Zhong, Ming1; Jiang, Xiao1; Zhang, Lvping1; Ren, Chunhua1; Hu, Chaoqun1; luoxingzky@163.com; chan1010@scsio.ac.cn; michell.mzhong@hotmail.com; jiangxiao4367@126.com; lpzhang@scsio.ac.cn; rosemary166@sina.com; cqhu@scsio.ac.cn
2015
发表期刊PEPTIDES
卷号68页码:58-63
摘要Molt-inhibiting hormone (MIH), a peptide member of the crustacean hyperglycemic hormone (CHH) family, is commonly considered as a negative regulator during the molt cycle in crustaceans. Phylogenetic analysis of CHH family peptides in penaeidae shrimps suggested that there is no significant differentiation between MIH and vitellogenesis-inhibiting hormone (VIH, another peptide member of CHH family), by far the most potent negative regulator of crustacean vitellogenesis known. Thus, MIH may also play a role in regulating vitellogenesis. In this study, two previously reported putative MIHs (LivMIH1 and LivMIH2) in the Pacific white shrimp (Litopenaeus vannamei) were expressed in Escherichia coli, purified by immobilized metal ion affinity chromatography (IMAC) and further confirmed by western blot. Regulation of vitellogenin (VTG) mRNA expression by recombinant LivMIH1 and LivMIH2 challenge was performed by both in vitro hepatopancreatic primary cells culture and in vivo injection approaches. In in vitro primary culture of shrimp hepatopancreatic cells, only LivMIH2 but not LivMIH1 administration could improve the mRNA expression of VTG. In in vivo injection experiments, similarly, only LivMIH2 but not LivMIH1 could stimulate hepatopancreatic VTG gene expression and induce ovary maturation. Our study may provide evidence for one isoform of MIH (MIH2 in L. vannamei) may serve as one of the mediators of the physiological progress of molting and vitellogenesis. Our study may also give new insight in CHH family peptides regulating reproduction in crustaceans, in particular penaeidae shrimps. (C) 2014 Elsevier Inc. All rights reserved.
部门归属LMB
关键词Mih Vih Litopenaeus Vannamei Vitellogenesis Reproduction Hepatopancreas
学科领域Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14828
专题中科院海洋生物资源可持续利用重点实验室
通讯作者luoxingzky@163.com; chan1010@scsio.ac.cn; michell.mzhong@hotmail.com; jiangxiao4367@126.com; lpzhang@scsio.ac.cn; rosemary166@sina.com; cqhu@scsio.ac.cn
作者单位1.[Luo, Xing
2.Chen, Ting
3.Zhong, Ming
4.Jiang, Xiao
5.Zhang, Lvping
6.Ren, Chunhua
7.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol LAMB, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou, Guangdong, Peoples R China
8.[Luo, Xing
9.Zhong, Ming] Univ Chinese Acad Sci, Beijing, Peoples R China
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Luo, Xing,Chen, Ting,Zhong, Ming,et al. Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)[J]. PEPTIDES,2015,68:58-63.
APA Luo, Xing.,Chen, Ting.,Zhong, Ming.,Jiang, Xiao.,Zhang, Lvping.,...&cqhu@scsio.ac.cn.(2015).Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei).PEPTIDES,68,58-63.
MLA Luo, Xing,et al."Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)".PEPTIDES 68(2015):58-63.
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