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Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121
Li, Lizhen1; Yang, Jian1; Li, Jie1; Long, Lijuan1; Xiao, Yunzhu1; Tian, Xinpeng1; Wang, Fazuo1; Zhang, Si1; yangjian@scsio.ac.cn; zhsimd@scsio.ac.cn
2015
Source PublicationBIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume38Issue:5Pages:871-879
Abstractalpha-Amylases from Bacillus licheniformis (BLA) and Bacillus amyloliquefaciens (BAA) are both important industrial enzymes with high similarity in structure but significant differences in thermostability. The mechanisms underlying this discrepancy are still poorly understood. Here, we investigated the role of two amino acids' insertion on the thermostability of these two group amylases. A newly obtained thermophilic amylase AMY121 was found much closer to BLA in both primary structure and enzymological properties. Two amino acids' insertion widespread among BAA group alpha-amylases was identified as one of the key factors leading to the thermostability differences, since thermostability of insertion mutants (AMY121-EG and AMY121-AA) from AMY121 significantly decreased, while that of deletion mutant from BAA increased. Moreover, we proposed that conformational disturbance caused by insertion mutation might weaken the calcium-binding affinity and consequently decrease the enzyme thermostability.
DepartmentLMB
KeywordAlpha-amylases Thermal Stability Site-directed Mutagenesis Calcium-binding Sites Deep Sea Bacterium
Subject AreaBiotechnology & Applied Microbiology ; Engineering
URL查看原文
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14839
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authoryangjian@scsio.ac.cn; zhsimd@scsio.ac.cn
Affiliation1.[Li, Lizhen
2.Yang, Jian
3.Li, Jie
4.Long, Lijuan
5.Xiao, Yunzhu
6.Tian, Xinpeng
7.Wang, Fazuo
8.Zhang, Si] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, RNAM Ctr Marine Microbiol,South China Sea Inst Oc, Guangzhou 510301, Guangdong, Peoples R China
9.[Li, Lizhen
10.Xiao, Yunzhu] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Li, Lizhen,Yang, Jian,Li, Jie,et al. Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(5):871-879.
APA Li, Lizhen.,Yang, Jian.,Li, Jie.,Long, Lijuan.,Xiao, Yunzhu.,...&zhsimd@scsio.ac.cn.(2015).Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121.BIOPROCESS AND BIOSYSTEMS ENGINEERING,38(5),871-879.
MLA Li, Lizhen,et al."Role of two amino acid residues' insertion on thermal stability of thermophilic alpha-amylase AMY121 from a deep sea bacterium Bacillus sp SCSIO 15121".BIOPROCESS AND BIOSYSTEMS ENGINEERING 38.5(2015):871-879.
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