Molecular cloning, characterization and expression analysis of PPAR gamma in the orange-spotted grouper (Epinephelus coioides) after the Vibrio algirtolyticus challenge
Luo, Shengwei1; Huang, Youhua1; Xie, Fuxing1; Huang, Xiaohong1; Liu, Yuan1; Wang, Weina1; Qin, Qiwei1; wangwn@scnu.edu.cn; qinqw@scsio.ac.cn
2015
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号43期号:2页码:310-324
摘要PPAR gamma was a key nuclear receptor, playing an important role in the immune defense and the anti-inflammatory mechanism. In this study, the full-length PPAR gamma (EcPPAR gamma) was obtained, containing a 5'UTR of 133 bp, an ORF of 1602 bp and a 3'UTR of 26 bp besides the poly (A) tail. The EcPPAR gamma gene encoded a protein of 533 amino acids with an estimated molecular mass of 60.02 KDa and a predicted isoelectric point (pI) of 6.26. The deduced amino acid sequence showed that EcPPAR gamma consisted of the conserved residues and the domains known to be critical for the PPAR gamma function. The quantitative real-time PCR analysis revealed that EcPPAR gamma transcript was expressed in all the examined tissue, while the strong expression was observed in intestine, followed by the expression in liver, gill, spleen heart, kidney and muscle. Vibrio challenge could stimulate the inflammatory response in grouper and induce a sharp increase of pro-inflammatory cytokines expression, lipid peroxidation and DNA damage, while the up-regulation of vibrio-induced inflammation could also increase the non-specific immune defense. The groupers challenged with Vibrio alginolyticus showed a sharp increase of EcPPAR gamma transcript in immune tissues. Subcellular localization analysis revealed that EcPPAR gamma was distributed in the nucleus. Furthermore, overexpression of EcPPAR gamma could down-regulated the expression of ILlb, IL6, TNF1 and TNF2. In addition, the administration of PPAR gamma antagonist, GW9662, could up-regulate the expression of pro-inflammatory genes, including ILlb, IL6, TNF1 and TNF2. Together, these results indicated that EcPPAR gamma serving as a negative regulator of pro-inflammatory cytokines may play an important role in the immune defense against vibrio-induced inflammation in grouper. (C) 2015 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Epinephelus Coioides Ppar Gamma Gene Expression Vibrio Alginolyticus
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14845
专题中科院海洋生物资源可持续利用重点实验室
通讯作者wangwn@scnu.edu.cn; qinqw@scsio.ac.cn
作者单位1.[Luo, Shengwei
2.Xie, Fuxing
3.Liu, Yuan
4.Wang, Weina] S China Normal Univ, Coll Life Sci, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Key Lab Ecol & Environm Sci Guangdong Higher Educ, Guangzhou 510631, Guangdong, Peoples R China
5.[Huang, Youhua
6.Huang, Xiaohong
7.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
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Luo, Shengwei,Huang, Youhua,Xie, Fuxing,et al. Molecular cloning, characterization and expression analysis of PPAR gamma in the orange-spotted grouper (Epinephelus coioides) after the Vibrio algirtolyticus challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):310-324.
APA Luo, Shengwei.,Huang, Youhua.,Xie, Fuxing.,Huang, Xiaohong.,Liu, Yuan.,...&qinqw@scsio.ac.cn.(2015).Molecular cloning, characterization and expression analysis of PPAR gamma in the orange-spotted grouper (Epinephelus coioides) after the Vibrio algirtolyticus challenge.FISH & SHELLFISH IMMUNOLOGY,43(2),310-324.
MLA Luo, Shengwei,et al."Molecular cloning, characterization and expression analysis of PPAR gamma in the orange-spotted grouper (Epinephelus coioides) after the Vibrio algirtolyticus challenge".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):310-324.
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