Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper
Wei, Jingguang1; Gao, Pin1; Zhang, Ping1; Guo, Minglan1; Xu, Meng1; Wei, Shina1; Yan, Yang1; Qin, Qiwei1; qinqw@scsio.ac.cn
2015
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号43期号:2页码:396-404
摘要Apolipoproteins, synthesized mainly in liver and intestine and bounded to lipids, play important roles in lipid transport and uptake through the circulation system. In this study, an apolipoprotein A-I gene homologue was cloned from orange-spotted grouper Epinephelus coioides (designed as Ec-ApoA-I) by rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of Ec-ApoA-I was comprised of 1278 bp with a 792 bp open reading frame (ORF) that encodes a putative protein of 264 amino acids. Quantitative real-time PCR (qPCR) analysis revealed that Ec-ApoA-I was abundant in liver and intestine, and the expression in liver was significantly (P < 0.01) up-regulated after the stimulation of LPS, Poly(I:C), Vibrio alginolyticus, and Singapore grouper iridovirus (SGIV). Recombinant Ec-ApoA-I (rEc-ApoA-I) was produced in Escherichia coli BL21 (DE3) expression system exhibited bacteriolyticactivity against Microcococcus lysodeikticus and Aeromonas hydrophila. Intracellular localization revealed that Ec-ApoA-I distributed in both cytoplasm and nucleus, and predominantly in the cytoplasm. Overexpression of Ec-ApoA-I in grouper Brain (GB) cells could inhibit the replication of SGIV. These results together indicated that Ec-ApoA-I perhaps is involved in the responses to bacterial and viral challenge. (C) 2015 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Epinephelus Coioides Apolipoprotein A-i Innate Immunity Intracellular Localization
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14846
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
作者单位1.[Wei, Jingguang
2.Guo, Minglan
3.Wei, Shina
4.Yan, Yang
5.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
6.[Wei, Jingguang
7.Guo, Minglan
8.Wei, Shina
9.Yan, Yang
10.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Guangdong, Peoples R China
11.[Gao, Pin
12.Xu, Meng] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
13.[Zhang, Ping] Sun Yat Sen Univ, Sch Life Sci, Teaching Ctr Biol Expt, Guangzhou 510275, Guangdong, Peoples R China
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Wei, Jingguang,Gao, Pin,Zhang, Ping,et al. Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper[J]. FISH & SHELLFISH IMMUNOLOGY,2015,43(2):396-404.
APA Wei, Jingguang.,Gao, Pin.,Zhang, Ping.,Guo, Minglan.,Xu, Meng.,...&qinqw@scsio.ac.cn.(2015).Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper.FISH & SHELLFISH IMMUNOLOGY,43(2),396-404.
MLA Wei, Jingguang,et al."Isolation and function analysis of apolipoprotein A-I gene response to virus infection in grouper".FISH & SHELLFISH IMMUNOLOGY 43.2(2015):396-404.
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