Analysis of DNA methylation in tissues and development stages of pearl oyster Pinctada fucata
Li, Yao-guo1; Guan, Yun-yan1; Li, Qin1; He, Mao-xian1; 13729887146@163.com; hmx@scsio.ac.cn
2015
发表期刊GENES & GENOMICS
卷号37期号:3页码:263-270
摘要In this study, we developed a methylation-sensitive amplification polymorphism technique to investigate DNA methylation profiles in different tissues and in the early-development stages of the pearl oyster Pinctada fucata (P. fucata). Methylation levels in adductor muscle, digestive gland, axe foot, heart, and gill ranged from 11.71 to 14.71 %, and significant differences (P < 0.05) between methylation levels in different tissues were observed. The DNA methylation levels of sperm, egg cells, two-cell embryos, morula embryos, trochophore larvae and D-shaped larvae were 13.51, 11.80, 12.14, 12.60, 14.65 and 13.18 %, respectively. Development stages of two-cell embryos, morula embryos, trochophore larvae and D-shaped larvae indicated a higher number of identical DNA methylation status loci in the egg, compared to that in the sperm. It is probable that DNA methylation patterns of the progeny are mainly influenced by the egg, while the sperm may become increasingly important during the process of early embryo development. The observed differences in methylation levels in the tissues and the development stages of P. fucata suggest that DNA methylation may act as an epigenetic regulator during tissue differentiation, individual growth, and development.
部门归属LMB
关键词Dna Methylation Msap Tissue Development Stage Pinctada Fucata
学科领域Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
URL查看原文
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14860
专题中科院海洋生物资源可持续利用重点实验室
通讯作者13729887146@163.com; hmx@scsio.ac.cn
作者单位1.[Li, Yao-guo
2.Guan, Yun-yan
3.Li, Qin
4.He, Mao-xian] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
5.[Li, Yao-guo
6.Li, Qin] Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Li, Yao-guo,Guan, Yun-yan,Li, Qin,et al. Analysis of DNA methylation in tissues and development stages of pearl oyster Pinctada fucata[J]. GENES & GENOMICS,2015,37(3):263-270.
APA Li, Yao-guo,Guan, Yun-yan,Li, Qin,He, Mao-xian,13729887146@163.com,&hmx@scsio.ac.cn.(2015).Analysis of DNA methylation in tissues and development stages of pearl oyster Pinctada fucata.GENES & GENOMICS,37(3),263-270.
MLA Li, Yao-guo,et al."Analysis of DNA methylation in tissues and development stages of pearl oyster Pinctada fucata".GENES & GENOMICS 37.3(2015):263-270.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Analysis of DNA meth(913KB)期刊论文作者接受稿开放获取CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。