Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production
Yang, Jian1; Li, Jie1; Hu, Yunfeng1; Li, Lizhen1; Long, Lijuan1; Wang, Fazuo1; Zhang, Si1; zhsimd@scsio.ac.cn
2015
发表期刊BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷号20期号:1页码:79-90
摘要High value utilization of byproduct of slaughterhouses is regarded as beneficial to both economy and environment. A hemoglobin degrading protease PA720 was purified from fermentation broth of a Porites lutea associated strain Streptomyces rutgersensis SCSIO 11720 (SCSIO 11720) and exhibited maximal activity at 70A degrees C, pH 10.5. The isolated strain was identified by genotypic method and 16 s RNA gene sequence was deposited in GenBank under accession No. KC414842. The enzyme exhibited excellent thermostability since it was stable at 70A degrees C with t (1/2) value of 1,386.3 min. Kinetic and thermodynamic study showed the activation energy (E (a) ), Delta G (*) (free energy), Delta H (*) (enthalpy) and Delta S (*) (entropy) for protease deactivation were 62.71 kJ/mol, 74.26 kJ/mol, 59.86 kJ/mol, and -41.97 J/mol/K, respectively. Highest specific activity of protease PA720 was observed when using casein (9,953 U/mg) and hemoglobin (9,854 U/mg) as substrates. Hemoglobin hydrolysate prepared by protease PA720 showed significant antibacterial activity towards Escherichia coli, Staphylococcus aureus and Bacillus subtilis, indicating this protease could be used as an instrumental enzyme for production of hemoglobin-based antibacterial peptides. Based on partial amino acid sequences of the enzyme PA720, the full gene encoding this protease was obtained by degenerate primer PCR and has been deposited in GenBank under accession No. KC414842. The deduced amino acid sequence exhibits homology with other microbial thermophilic proteases in subtilisin family.
部门归属LMB
关键词Hemoglobin Protease Thermophilic Enzyme Enzyme Purification Antibacterial Peptides Streptomyces Isolation And Identification
学科领域Biotechnology & Applied Microbiology
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14867
专题中科院海洋生物资源可持续利用重点实验室
通讯作者zhsimd@scsio.ac.cn
作者单位1.[Yang, Jian
2.Li, Jie
3.Hu, Yunfeng
4.Li, Lizhen
5.Long, Lijuan
6.Wang, Fazuo
7.Zhang, Si] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, RNAM Ctr Marine Microbiol,Guangdong Key Lab Marin, Guangzhou 510301, Guangdong, Peoples R China
8.[Li, Lizhen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Yang, Jian,Li, Jie,Hu, Yunfeng,et al. Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production[J]. BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,2015,20(1):79-90.
APA Yang, Jian.,Li, Jie.,Hu, Yunfeng.,Li, Lizhen.,Long, Lijuan.,...&zhsimd@scsio.ac.cn.(2015).Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production.BIOTECHNOLOGY AND BIOPROCESS ENGINEERING,20(1),79-90.
MLA Yang, Jian,et al."Characterization of a thermophilic hemoglobin-degrading protease from Streptomyces rutgersensis SCSIO 11720 and its application in antibacterial peptides production".BIOTECHNOLOGY AND BIOPROCESS ENGINEERING 20.1(2015):79-90.
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