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Molecular Characterization of dax1 and SF-1 and Their Expression Analysis During Sex Reversal in Spotted Scat, Scatophagus argus
Chen, Jianhua1; He, Maoxian1; Yan, Binlun1; Zhang, Junbin1; Jin, Sichen1; Liu, Lu1; jbzhang30@163.com
2015
Source PublicationJOURNAL OF THE WORLD AQUACULTURE SOCIETY
Volume46Issue:1Pages:1-19
AbstractThe nuclear receptors dax1 and SF-1 play important and antagonistic roles in gonad differentiation in mammals. To study their potential functions in teleost fish, the full-length cDNAs of dax1 and SF-1 were cloned and characterized from spotted scat. Their expression patterns in adult tissues and juveniles treated with 17-methyltestosterone (MT) and their gonadal histological changes were investigated, respectively. The tissue-specific expression results showed that dax1 was abundantly expressed in the gonads, spleen, brain, and intestine. SF-1 was preferentially expressed in the gonad and spleen. Histological results showed that sex reversal could be induced by MT, and the degenerating oocytes and proliferations of spermatogonia were observed in gonad. At the beginning of sex reversal (40d after treatment), the mRNA level of dax1 significantly increased, and the transcript level of SF-1mRNA was inhibited at the point of 20 d following treatment with MT. Our results suggested that masculinization could be induced by MT treatment in spotted scat, and MT could upregulate the dax1 expression, whereas it downregulates the expression of SF-1, which implied that dax1 and SF-1 might be involved in the gonad development from female to male and there was an antagonistic effect between dax1 and SF-1.
DepartmentLMB
Subject AreaFisheries
URL查看原文
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14869
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorjbzhang30@163.com
Affiliation1.[Chen, Jianhua
2.Zhang, Junbin] Shanghai Ocean Univ, Coll Aquaculture & Life Sci, Shanghai 201306, Peoples R China
3.[Chen, Jianhua
4.He, Maoxian] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
5.[Chen, Jianhua
6.Yan, Binlun
7.Jin, Sichen
8.Liu, Lu] Huaihai Inst Technol, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China
9.[Chen, Jianhua] Huaihai Inst Technol, Jiangsu Prov R&D Inst Marine Resources, Lianyungang 222005, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jianhua,He, Maoxian,Yan, Binlun,et al. Molecular Characterization of dax1 and SF-1 and Their Expression Analysis During Sex Reversal in Spotted Scat, Scatophagus argus[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2015,46(1):1-19.
APA Chen, Jianhua.,He, Maoxian.,Yan, Binlun.,Zhang, Junbin.,Jin, Sichen.,...&jbzhang30@163.com.(2015).Molecular Characterization of dax1 and SF-1 and Their Expression Analysis During Sex Reversal in Spotted Scat, Scatophagus argus.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,46(1),1-19.
MLA Chen, Jianhua,et al."Molecular Characterization of dax1 and SF-1 and Their Expression Analysis During Sex Reversal in Spotted Scat, Scatophagus argus".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 46.1(2015):1-19.
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