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ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis
Xiang, Zhiming1; Qu, Fufa1; Wang, Fuxuan1; Xiao, Shu1; Jun, Li1; Zhang, Yang1; Yu, Ziniu1; carlzyu@scsio.ac.cn
2015
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume42Issue:2Pages:225-232
AbstractApoptosis has been primarily investigated in mammals, and little is known about apoptosis in mollusks. The proteins Bax and Bak play critical roles in the mitochondria] apoptosis pathway and in determining cell fate. In this study, ChBax and ChBak, homologs of the well-known Bax and Bak proteins, were identified from the oyster Crassostrea hongkongensis. The ChBax/Bak proteins consist of 207/232 amino acids with the typical domains found in BCL-2 family members. ChBax and ChBak mRNA expression were detected in all 8 of the selected oyster tissues and at the different stages of development. Fluorescence microscopy revealed that the full-length proteins of ChBax/Bak were located in the cytoplasm and mitochondrial outer membrane, of HEK293T cells, respectively. Furthermore, both of the genes' expression levels were found to increase in the hemocytes of oysters challenged with pathogens. The over-expression of ChBax or ChBak activates the p53-Luc reporter gene in HEK293T cells in a dose-dependent manner. These results indicate that ChBax and ChBak may play important roles in the mitochondrial apoptotic pathway in oysters. (C) 2014 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordCrassostrea Hongkongensis Mitochondrial Pathway Chbax Chbak
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14872
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorcarlzyu@scsio.ac.cn
Affiliation1.[Xiang, Zhiming
2.Qu, Fufa
3.Wang, Fuxuan
4.Xiao, Shu
5.Jun, Li
6.Zhang, Yang
7.Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
8.[Qu, Fufa
9.Wang, Fuxuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Xiang, Zhiming,Qu, Fufa,Wang, Fuxuan,et al. ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(2):225-232.
APA Xiang, Zhiming.,Qu, Fufa.,Wang, Fuxuan.,Xiao, Shu.,Jun, Li.,...&carlzyu@scsio.ac.cn.(2015).ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,42(2),225-232.
MLA Xiang, Zhiming,et al."ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 42.2(2015):225-232.
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