ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis
Xiang, Zhiming1; Qu, Fufa1; Wang, Fuxuan1; Xiao, Shu1; Jun, Li1; Zhang, Yang1; Yu, Ziniu1; carlzyu@scsio.ac.cn
2015
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号42期号:2页码:225-232
摘要Apoptosis has been primarily investigated in mammals, and little is known about apoptosis in mollusks. The proteins Bax and Bak play critical roles in the mitochondria] apoptosis pathway and in determining cell fate. In this study, ChBax and ChBak, homologs of the well-known Bax and Bak proteins, were identified from the oyster Crassostrea hongkongensis. The ChBax/Bak proteins consist of 207/232 amino acids with the typical domains found in BCL-2 family members. ChBax and ChBak mRNA expression were detected in all 8 of the selected oyster tissues and at the different stages of development. Fluorescence microscopy revealed that the full-length proteins of ChBax/Bak were located in the cytoplasm and mitochondrial outer membrane, of HEK293T cells, respectively. Furthermore, both of the genes' expression levels were found to increase in the hemocytes of oysters challenged with pathogens. The over-expression of ChBax or ChBak activates the p53-Luc reporter gene in HEK293T cells in a dose-dependent manner. These results indicate that ChBax and ChBak may play important roles in the mitochondrial apoptotic pathway in oysters. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Crassostrea Hongkongensis Mitochondrial Pathway Chbax Chbak
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14872
专题中科院海洋生物资源可持续利用重点实验室
通讯作者carlzyu@scsio.ac.cn
作者单位1.[Xiang, Zhiming
2.Qu, Fufa
3.Wang, Fuxuan
4.Xiao, Shu
5.Jun, Li
6.Zhang, Yang
7.Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
8.[Qu, Fufa
9.Wang, Fuxuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Xiang, Zhiming,Qu, Fufa,Wang, Fuxuan,et al. ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(2):225-232.
APA Xiang, Zhiming.,Qu, Fufa.,Wang, Fuxuan.,Xiao, Shu.,Jun, Li.,...&carlzyu@scsio.ac.cn.(2015).ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis.FISH & SHELLFISH IMMUNOLOGY,42(2),225-232.
MLA Xiang, Zhiming,et al."ChBax/Bak as key regulators of the mitochondrial apoptotic pathway: Cloned and characterized in Crassostrea hongkongensis".FISH & SHELLFISH IMMUNOLOGY 42.2(2015):225-232.
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