Identification of orange-spotted grouper (Epinephelus coioides) interferon regulatory factor 3 involved in antiviral immune response against fish RNA virus
Huang, Youhua1; Huang, Xiaohong1; Cai, Jia1; OuYang, Zhengliang1; Wei, Shina1; Wei, Jingguang1; Qin, Qiwei1; qinqw@scsio.ac.cn
2015
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号42期号:2页码:345-352
摘要Interferon regulatory factor 3 (IRF3) is an important transcription factor which regulates the expression of interferon (IFN) and IFN-stimulated genes (ISGs) following virus recognition. In this study, a novel IRF3 gene was cloned from grouper Epinephelus coioides (EcIRF3) and its effects against Singapore grouper iridovirus (SGIV) and red spotted grouper nervous necrosis virus (RGNNV) was investigated. The full-length of EcIRF3 cDNA was composed of 2513 bp and encoded a polypeptide of 458 amino acids which shared 82% identity with European seabass (Dicentrarchus labrax). EcIRF3 contained three conserved domains including a DNA-binding domain (DBD), an IRF associated domain (IAD) and a serine-rich domain. Expression profile analysis revealed that EcIRF3 was abundant in head kidney, kidney, spleen and gill. Upon different stimuli in vitro, the transcript of EcIRF3 was significantly up-regulated after RGNNV infection or treatment with polyinosin-polycytidylic acid (poly I:C). During SGIV infection, the increase of the EcIRF3 transcription was only detected at the late stage, suggesting that EcIRF3 was differently regulated by different stimuli. Immune fluorescence assay indicated that the fluorescence signal of EcIRF3 was increased significantly after infection with RGNNV or treatment with poly I:C, but moderately at the late stage of SGIV infection. Reporter gene assay showed that EcIRF3 activated zebrafish type I IFN and type III IFN promoter in vitro. The viral gene transcription and virus production of RGNNV were significantly decreased in EcIRF3 overexpressing cells. However, the ectopic expression of EcIRF3 did not affect the gene transcription and virus production of SGIV. Moreover, the mRNA expression levels of type I IFN and IFN-inducible genes (MxI, ISG15 and ISG56) were increased in RGNNV infected EcIRF3 overexpressing cells compared to empty vector transfected cells. Together, our results demonstrated that IFN immune response mediated by grouper IRF3 was exerted crucial roles for fish RNA virus, but not for DNA virus replication. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Irf3 Grouper Rgnnv Sgiv Virus Replication
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14874
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qinqw@scsio.ac.cn
作者单位1.[Huang, Youhua
2.Huang, Xiaohong
3.Cai, Jia
4.OuYang, Zhengliang
5.Wei, Shina
6.Wei, Jingguang
7.Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
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Huang, Youhua,Huang, Xiaohong,Cai, Jia,et al. Identification of orange-spotted grouper (Epinephelus coioides) interferon regulatory factor 3 involved in antiviral immune response against fish RNA virus[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(2):345-352.
APA Huang, Youhua.,Huang, Xiaohong.,Cai, Jia.,OuYang, Zhengliang.,Wei, Shina.,...&qinqw@scsio.ac.cn.(2015).Identification of orange-spotted grouper (Epinephelus coioides) interferon regulatory factor 3 involved in antiviral immune response against fish RNA virus.FISH & SHELLFISH IMMUNOLOGY,42(2),345-352.
MLA Huang, Youhua,et al."Identification of orange-spotted grouper (Epinephelus coioides) interferon regulatory factor 3 involved in antiviral immune response against fish RNA virus".FISH & SHELLFISH IMMUNOLOGY 42.2(2015):345-352.
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