Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility
Guo, C. Y.1; Huang, Y. H.1; Wei, S. N.1; Ouyang, Z. L.1; Yan, Y.1; Huang, X. H.1; Qin, Q. W.1; huangxh@scsio.ac.cn; qinqw@scsio.ac.cn
2015
发表期刊JOURNAL OF FISH DISEASES
卷号38期号:2页码:175-186
摘要A new marine fish cell line, derived from the heart of giant grouper, Epinephelus lanceolatus (Bloch), was established and characterized. The cell line was designated as ELGH and subcultured with more than 60 passages. The ELGH cells were mainly composed of fibroblast-like cells and multiplied well in Leibovitz's L-15 medium supplemented with 10% foetal bovine serum (FBS) at 28 degrees C. Chromosome analysis indicated that the modal chromosome number was 48. The fluorescent signals were detected in ELGH when transfected with green fluorescent protein reporter plasmids. The 50% cytotoxic concentration (CC50) of the extracellular products (ECPs) from Streptococcus iniae and Vibrio alginolyticus E333 on ELGH cells was 60.02 and 12.49gmL(-1), respectively. Moreover, the ELGH cells showed susceptibility to Singapore grouper iridovirus (SGIV), but not to soft-shelled turtle iridovirus (STIV), red-spotted grouper nervous necrosis virus (RGNNV) and spring viremia of carp virus (SVCV), which was demonstrated by the presence of a severe cytopathic effect (CPE) and increased viral titres. In addition, electron microscopy observation showed that abundant virus particles were present in the infected cells. Taken together, our data above provided the potential utility of ELGH cells for transgenic and genetic manipulation, as well as cytotoxicity testing and virus pathogenesis.
部门归属LMB
关键词Cytotoxicity Elgh Epinephelus Lanceolatus Transgenic Manipulation Virus Sensitivity
学科领域Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
URL查看原文
文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14876
专题中科院海洋生物资源可持续利用重点实验室
通讯作者huangxh@scsio.ac.cn; qinqw@scsio.ac.cn
作者单位1.[Guo, C. Y.
2.Huang, Y. H.
3.Wei, S. N.
4.Ouyang, Z. L.
5.Yan, Y.
6.Huang, X. H.
7.Qin, Q. W.] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
8.[Guo, C. Y.] Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Guo, C. Y.,Huang, Y. H.,Wei, S. N.,et al. Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility[J]. JOURNAL OF FISH DISEASES,2015,38(2):175-186.
APA Guo, C. Y..,Huang, Y. H..,Wei, S. N..,Ouyang, Z. L..,Yan, Y..,...&qinqw@scsio.ac.cn.(2015).Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility.JOURNAL OF FISH DISEASES,38(2),175-186.
MLA Guo, C. Y.,et al."Establishment of a new cell line from the heart of giant grouper, Epinephelus lanceolatus (Bloch), and its application in toxicology and virus susceptibility".JOURNAL OF FISH DISEASES 38.2(2015):175-186.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。