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Cloning and differential expression of Na,K-ATPase in Penaeus vannamei
Sun, Hongyan1; Dong, Junde1; Ren, Chunhua1; Zhang, Lvping1; Dan, Xueming1; Chen, Chang1; Zhang, Yuanzhou1; Hu, Chaoqun1;
AbstractTo better understand the adaptive strategies of osmotic and ionic regulation in Penaeus vannamei, a full-length cDNA sequence encoding the Na,K-ATPase alpha subunit was isolated and characterized by rapid amplification of the cDNA ends (RACE). The nucleotide sequence was 3953 bp in length and included a 300 bp 5 untranslated region (UTR), 536 bp 3 UTR with an AATAA canonical polyadenylation signal sequence and poly (A) tail, and a 3117 bp open reading frame (ORF) encoding a 1038 amino acid protein. The calculated molecular weight of the protein was 115.4 kDa with an estimated pI of 5.29. This sequence was submitted to the National Center for Biotechnology Information (NCBI) GenBank under accession number KF765670. A phenetic analysis showed that the Na,K-ATPase -subunit of P. vannamei shared a high degree of identity with other species. The relevant mRNA was detected in all of the tissues examined by fluorescent quantitative real-time polymerase chain reaction. The expression level was higher in the gills and hepatopancreas, and lower in the guts, muscles and epithelia. Increased expression of Na,K-ATPase mRNA levels was observed in all of the tissues examined in response to salinity exposure, and there was a significant difference in the tissues of the gills and hepatopancreas (P < 0.05); however, no statistical difference was observed in the epithelia, guts and muscles compared with the controls (P > 0.05). The findings demonstrated that the Na,K-ATPase -subunit is highly conserved in crustaceans, and differential expression in different tissues of P. vannamei was observed, indicating that the gene might have multiple functions in the shrimp.
KeywordDifferential Expression K-atpase Molecular Cloning Na Penaeus Vannamei Race
Subject AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
Document Type期刊论文
Affiliation1.[Sun, Hongyan
2.Dong, Junde
3.Ren, Chunhua
4.Zhang, Lvping
5.Chen, Chang
6.Zhang, Yuanzhou
7.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol LMB, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou, Guangdong, Peoples R China
8.[Sun, Hongyan
9.Dong, Junde
10.Ren, Chunhua
11.Zhang, Lvping
12.Chen, Chang
13.Zhang, Yuanzhou
14.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, LAMB, Guangzhou, Guangdong, Peoples R China
15.[Sun, Hongyan] Chinese Acad Sci, Natl Expt Stn Trop Marine Biol, Sanya, Peoples R China
16.[Sun, Hongyan
17.Dan, Xueming] South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Sun, Hongyan,Dong, Junde,Ren, Chunhua,et al. Cloning and differential expression of Na,K-ATPase in Penaeus vannamei[J]. MARINE BIOLOGY RESEARCH,2015,11(9):983-989.
APA Sun, Hongyan.,Dong, Junde.,Ren, Chunhua.,Zhang, Lvping.,Dan, Xueming.,...& and differential expression of Na,K-ATPase in Penaeus vannamei.MARINE BIOLOGY RESEARCH,11(9),983-989.
MLA Sun, Hongyan,et al."Cloning and differential expression of Na,K-ATPase in Penaeus vannamei".MARINE BIOLOGY RESEARCH 11.9(2015):983-989.
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