Knowledge Management System Of South China Sea Institute of Oceanology,CAS
| Cloning and differential expression of Na,K-ATPase in Penaeus vannamei | |
| Sun, Hongyan1; Dong, Junde1; Ren, Chunhua1; Zhang, Lvping1; Dan, Xueming1; Chen, Chang1; Zhang, Yuanzhou1; Hu, Chaoqun1; cqhu@scsio.ac.cn | |
| 2015 | |
| Source Publication | MARINE BIOLOGY RESEARCH
 |
| Volume | 11Issue:9Pages:983-989 |
| Abstract | To better understand the adaptive strategies of osmotic and ionic regulation in Penaeus vannamei, a full-length cDNA sequence encoding the Na,K-ATPase alpha subunit was isolated and characterized by rapid amplification of the cDNA ends (RACE). The nucleotide sequence was 3953 bp in length and included a 300 bp 5 untranslated region (UTR), 536 bp 3 UTR with an AATAA canonical polyadenylation signal sequence and poly (A) tail, and a 3117 bp open reading frame (ORF) encoding a 1038 amino acid protein. The calculated molecular weight of the protein was 115.4 kDa with an estimated pI of 5.29. This sequence was submitted to the National Center for Biotechnology Information (NCBI) GenBank under accession number KF765670. A phenetic analysis showed that the Na,K-ATPase -subunit of P. vannamei shared a high degree of identity with other species. The relevant mRNA was detected in all of the tissues examined by fluorescent quantitative real-time polymerase chain reaction. The expression level was higher in the gills and hepatopancreas, and lower in the guts, muscles and epithelia. Increased expression of Na,K-ATPase mRNA levels was observed in all of the tissues examined in response to salinity exposure, and there was a significant difference in the tissues of the gills and hepatopancreas (P < 0.05); however, no statistical difference was observed in the epithelia, guts and muscles compared with the controls (P > 0.05). The findings demonstrated that the Na,K-ATPase -subunit is highly conserved in crustaceans, and differential expression in different tissues of P. vannamei was observed, indicating that the gene might have multiple functions in the shrimp. |
| Department | LMB |
| Keyword | Differential Expression K-atpase Molecular Cloning Na Penaeus Vannamei Race |
| Subject Area | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| URL | 查看原文 |
| Document Type | 期刊论文 |
| Identifier | http://ir.scsio.ac.cn/handle/344004/14888 |
| Collection | 中科院海洋生物资源可持续利用重点实验室 |
| Corresponding Author | cqhu@scsio.ac.cn |
| Affiliation | 1.[Sun, Hongyan 2.Dong, Junde 3.Ren, Chunhua 4.Zhang, Lvping 5.Chen, Chang 6.Zhang, Yuanzhou 7.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol LMB, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou, Guangdong, Peoples R China 8.[Sun, Hongyan 9.Dong, Junde 10.Ren, Chunhua 11.Zhang, Lvping 12.Chen, Chang 13.Zhang, Yuanzhou 14.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, LAMB, Guangzhou, Guangdong, Peoples R China 15.[Sun, Hongyan] Chinese Acad Sci, Natl Expt Stn Trop Marine Biol, Sanya, Peoples R China 16.[Sun, Hongyan 17.Dan, Xueming] South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China |
| Recommended Citation GB/T 7714 | Sun, Hongyan,Dong, Junde,Ren, Chunhua,et al. Cloning and differential expression of Na,K-ATPase in Penaeus vannamei[J]. MARINE BIOLOGY RESEARCH,2015,11(9):983-989. |
| APA | Sun, Hongyan.,Dong, Junde.,Ren, Chunhua.,Zhang, Lvping.,Dan, Xueming.,...&cqhu@scsio.ac.cn.(2015).Cloning and differential expression of Na,K-ATPase in Penaeus vannamei.MARINE BIOLOGY RESEARCH,11(9),983-989. |
| MLA | Sun, Hongyan,et al."Cloning and differential expression of Na,K-ATPase in Penaeus vannamei".MARINE BIOLOGY RESEARCH 11.9(2015):983-989. |
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