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Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode
Gu, Binbin1; Zhang, Yanying1; Ding, Lijian1; He, Shan1; Wu, Bin1; Dong, Junde1; Zhu, Peng1; Chen, Juanjuan1; Zhang, Jinrong1; Yan, Xiaojun1; gubin21314@gmail.com; zyy@scsio.ac.cn; huahua20062008@126.com; heshan@nbu.edu.cn; wubin@zju.edu.cn; dongjunde@vip.163.com; zhupeng@nbu.edu.cn; chenjuanjuan@nbu.edu.cn; zhangjinrong@nbu.edu.cn; yanxiaojun@nbu.edu.cn
2015
Source PublicationMARINE DRUGS
Volume13Issue:1Pages:354-365
AbstractHigh-speed counter-current chromatography (HSCCC) was successively applied to the separation of three sulfur-containing diketopiperazines (DKPs) (including two new compounds cladosporin A (1) and cladosporin B (3), and a known compound haematocin (2)) from a marine fungus Cladosporium sp. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water at (1:1:1:1, v/v) and (2:1:2:1, v/v), in stepwise elution mode, was used for HSCCC. The preparative HSCCC separation was performed on 300 mg of crude sample yielding 26.7 mg of compound 3 at a purity of over 95%, 53.6 mg of a mixture of compounds 1 and 2, which was further separated by preparative-HPLC yielding 14.3 mg of compound 1 and 25.4 mg of compound 2 each at a purity of over 95%. Their structures were established by spectroscopic methods. The sulfur-containing DKPs suppressed the proliferation of hepatocellular carcinoma cell line HepG2. The present work represents the first application of HSCCC in the efficient preparation of marine fungal natural products.
DepartmentLMB
KeywordCladosporium High-speed Counter-current Chromatography Marine Fungus Sulfur-containing Diketopiperazines
Subject AreaPharmacology & Pharmacy
URL查看原文
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14895
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorgubin21314@gmail.com; zyy@scsio.ac.cn; huahua20062008@126.com; heshan@nbu.edu.cn; wubin@zju.edu.cn; dongjunde@vip.163.com; zhupeng@nbu.edu.cn; chenjuanjuan@nbu.edu.cn; zhangjinrong@nbu.edu.cn; yanxiaojun@nbu.edu.cn
Affiliation1.[Gu, Binbin
2.He, Shan
3.Chen, Juanjuan
4.Zhang, Jinrong] Ningbo Univ, Sch Marine Sci, Lab Marine Nat Prod, Ningbo 315211, Zhejiang, Peoples R China
5.[Zhang, Yanying
6.Dong, Junde] Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
7.[Ding, Lijian] Jinan Univ, Coll Pharm, Guangzhou 510632, Guangdong, Peoples R China
8.[Wu, Bin] Zhejiang Univ, Ocean Coll, Hangzhou 310058, Zhejiang, Peoples R China
9.[Zhu, Peng
10.Yan, Xiaojun] Ningbo Univ, Minist Educ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
Recommended Citation
GB/T 7714
Gu, Binbin,Zhang, Yanying,Ding, Lijian,et al. Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode[J]. MARINE DRUGS,2015,13(1):354-365.
APA Gu, Binbin.,Zhang, Yanying.,Ding, Lijian.,He, Shan.,Wu, Bin.,...&yanxiaojun@nbu.edu.cn.(2015).Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode.MARINE DRUGS,13(1),354-365.
MLA Gu, Binbin,et al."Preparative Separation of Sulfur-Containing Diketopiperazines from Marine Fungus Cladosporium sp Using High-Speed Counter-Current Chromatography in Stepwise Elution Mode".MARINE DRUGS 13.1(2015):354-365.
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