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The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus)
Ren, Chunhua1; Chen, Ting1; Jiang, Xiao1; Luo, Xing1; Wang, Yanhong1; Hu, Chaoqun1; rosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; luoxingzky@163.com; yhwang@scsio.ac.cn; hucq@scsio.ac.cn
2015
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume42Issue:1Pages:41-49
AbstractGamma-interferon-inducible lysosomal thiol reductase (GILT) has been described as a key enzyme that facilitating the processing and presentation of major histocompatibility complex class II-restricted antigen in mammals. In this study, the first echinoderm GILT named StmGILT was identified from sea cucumber (Stichopus monotuberculatus). The StmGILT cDNA is 1529 bp in length, containing a 5'-un-translated region (UTR) of 87 bp, a 3'-UTR of 674 bp and an open reading frame (ORF) of 768 bp that encoding a protein of 255 amino acids with a deduced molecular weight of 27.82 kDa and a predicted isoelectric point of 4.73. The putative StmGILT protein possesses all the main characteristics of known GILT proteins, including a signature sequence, a reductase active site CXXC, twelve conserved cysteines, and two potential N-linked glycosylation sites. For the gene structure, StmGILT contains four exons separated by three introns. In the promoter region of StmGILT gene, an NF-kappa B binding site and an IFN-gamma activation site were found. The thiol reductase activity of recombinant StmGILT protein was also demonstrated in this study. In addition, the highest level of mRNA expression was noticed in coelomocytes of S. monotuberculatus. In in vitro experiments performed in coelomocytes, the expression of StmGILT mRNA was significantly up-regulated by lipopolysaccharides (LPS), inactivated bacteria or polyriboinosinic polyribocytidylic acid [poly (LC)] challenge, suggested that the sea cucumber GILT might play critical roles in the innate immune defending against bacterial and viral infections. (C) 2014 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordGamma-interferon-inducible Lysosomal Thiol Reductase Stichopus Monotuberculatus Gene Structure Thiol Reductase Activity Innate Immune
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14897
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorrosemary166@sina.com; chan1010@scsio.ac.cn; jiangxiao4367@126.com; luoxingzky@163.com; yhwang@scsio.ac.cn; hucq@scsio.ac.cn
Affiliation1.[Ren, Chunhua
2.Chen, Ting
3.Jiang, Xiao
4.Luo, Xing
5.Wang, Yanhong
6.Hu, Chaoqun] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Appl Marine Biol Guangdong Prov, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Ren, Chunhua,Chen, Ting,Jiang, Xiao,et al. The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus)[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(1):41-49.
APA Ren, Chunhua.,Chen, Ting.,Jiang, Xiao.,Luo, Xing.,Wang, Yanhong.,...&hucq@scsio.ac.cn.(2015).The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus).FISH & SHELLFISH IMMUNOLOGY,42(1),41-49.
MLA Ren, Chunhua,et al."The first echinoderm gamma-interferon-inducible lysosomal thiol reductase (GILT) identified from sea cucumber (Stichopus monotuberculatus)".FISH & SHELLFISH IMMUNOLOGY 42.1(2015):41-49.
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