SCSIO OpenIR  > 中科院海洋生物资源可持续利用重点实验室
Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization
Huang, Xian-De1; Wei, Guo-jian1; Zhang, Hua1; He, Mao-Xian1; hmx@scsio.ac.cn
2015
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume42Issue:1Pages:108-113
AbstractNuclear factor of activated T cells (NFAT) plays an important role in nonimmune cells and also in T cells and many other cells of the immune system, by regulating the expression of a variety of genes involved in the immune response, organ development, developmental apoptosis and angiogenesis. In the present study, the NFAT homology gene, PfNFAT, from the pearl oyster Pinctada fucata was cloned and its genomic structure and promoter were analyzed. PfNFAT encodes a putative protein of 1226 amino acids, and contains a highly conserved Rel homology region (RHR) with DNA-binding specificity, and a regulatory domain (NFAT homology region, NHR) containing a potent transactivation domain (TAD). The PfNFAT gene consists of 12 exons and 11 introns, and its promoter contains potential binding sites for transcription factors such as NF-kappa B (Nuclear factor kappa B), STATx (signal transducer and activator of transcription), AP-1 (activator protein-1) and Sox-5/9 (SRY type HMG box-5/9), MyoD (Myogenic Differentiation Antigen) and IRF (Interferon regulatory factor). Comparison and phylogenetic analysis revealed that PfNFAT shows high identity with other invertebrate NFAT, and clusters with the NFAT5 subgroup. Furthermore, gene expression analysis revealed that PfNFAT is involved in the immune response to lipopolysaccharide (LPS) and Polyinosinic-polycytidylic acid (poly I:C) stimulation and in the nucleus inserting operation. The study of PfNFAT may increase understanding of molluscan innate immunity. (C) 2014 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordPearl Oyster Pfnfat Gene Cloning Gene Expression
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
URL查看原文
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14898
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorhmx@scsio.ac.cn
Affiliation1.[Huang, Xian-De
2.Wei, Guo-jian
3.Zhang, Hua
4.He, Mao-Xian] Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, South China Sea Inst Oceanol, Guangzhou 510301, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Huang, Xian-De,Wei, Guo-jian,Zhang, Hua,et al. Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(1):108-113.
APA Huang, Xian-De,Wei, Guo-jian,Zhang, Hua,He, Mao-Xian,&hmx@scsio.ac.cn.(2015).Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization.FISH & SHELLFISH IMMUNOLOGY,42(1),108-113.
MLA Huang, Xian-De,et al."Nuclear factor of activated T cells (NFAT) in pearl oyster Pinctada fucata: Molecular cloning and functional characterization".FISH & SHELLFISH IMMUNOLOGY 42.1(2015):108-113.
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