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Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei
Chen, Ting1; Wong, Nai-Kei1; Jiang, Xiao1; Luo, Xing1; Zhang, Lvping1; Yang, Dan1; Ren, Chunhua1; Hu, Chaoqun1; chan1010@scsio.ac.cn; wongnk@gmail.com; jiangxiao4367@126.com; luoxingzky@163.com; lpzhang@scsio.ac.cn; yangdan@hku.hk; rosemary166@sina.com; hucq@scsio.ac.cn
2015
Source PublicationFISH & SHELLFISH IMMUNOLOGY
Volume42Issue:1Pages:114-120
AbstractNitric oxide (NO) is a key effector molecule produced in the innate immune systems of many species for antimicrobial defense. However, how NO production is regulated during bacterial infection in invertebrates, especially crustaceans, remains poorly understood. Vibrio harveyi, a Gram-negative marine pathogen, is among the most prevalent and serious threats to the world's shrimp culture industry. Its virulence typically manifests itself through shrimp hepatopancreas destruction. In the current study, we found that NO generated by an in vitro donor system (NOC-18) could rapidly and effectively kill V harveyi. In addition, injection of heat-killed V. harveyi increased the concentration of NO/nitrite and the mRNA expression of nitric oxide synthase (NOS) in the hepatopancreas of Pacific white shrimp (Litopenaeus vannamei), the commercially most significant shrimp species. Live V. harveyi challenge also induced NO/nitrite production and NOS gene expression in primary L. vannamei hepatopancreatic cells in a time- and dose-dependent manner. Co-incubation of L-NAME, an inhibitor selective for mammalian constitutive NOSs, dose-dependently blocked V harveyi-induced NO/nitrite production, without affecting V harveyi-induced NOS mRNA expression. Furthermore, L-NAME treatment significantly increased the survival rate of infecting V harveyi in cultured primary hepatopancreatic cells of L. vannamei. As a whole, we have demonstrated that endogenous NO produced by L. vannamei hepatopancreatic cells occurs in enzymatically regulated manners and is sufficient to act as a bactericidal molecule for V. harveyi clearance. (C) 2014 Elsevier Ltd. All rights reserved.
DepartmentLMB
KeywordNitric Oxide Antimicrobial Molecule Vibrio Harveyi Litopenaeus Vannamei Hepatopanfcreas
Subject AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14899
Collection中科院海洋生物资源可持续利用重点实验室
Corresponding Authorchan1010@scsio.ac.cn; wongnk@gmail.com; jiangxiao4367@126.com; luoxingzky@163.com; lpzhang@scsio.ac.cn; yangdan@hku.hk; rosemary166@sina.com; hucq@scsio.ac.cn
Affiliation1.[Chen, Ting
2.Jiang, Xiao
3.Luo, Xing
4.Zhang, Lvping
5.Ren, Chunhua
6.Hu, Chaoqun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Key Lab Appl Marine Biol Guangdong Prov, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
7.[Wong, Nai-Kei
8.Yang, Dan] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
9.[Luo, Xing] Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Chen, Ting,Wong, Nai-Kei,Jiang, Xiao,et al. Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(1):114-120.
APA Chen, Ting.,Wong, Nai-Kei.,Jiang, Xiao.,Luo, Xing.,Zhang, Lvping.,...&hucq@scsio.ac.cn.(2015).Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,42(1),114-120.
MLA Chen, Ting,et al."Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 42.1(2015):114-120.
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