Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei
Chen, Ting1; Wong, Nai-Kei1; Jiang, Xiao1; Luo, Xing1; Zhang, Lvping1; Yang, Dan1; Ren, Chunhua1; Hu, Chaoqun1; chan1010@scsio.ac.cn; wongnk@gmail.com; jiangxiao4367@126.com; luoxingzky@163.com; lpzhang@scsio.ac.cn; yangdan@hku.hk; rosemary166@sina.com; hucq@scsio.ac.cn
2015
发表期刊FISH & SHELLFISH IMMUNOLOGY
卷号42期号:1页码:114-120
摘要Nitric oxide (NO) is a key effector molecule produced in the innate immune systems of many species for antimicrobial defense. However, how NO production is regulated during bacterial infection in invertebrates, especially crustaceans, remains poorly understood. Vibrio harveyi, a Gram-negative marine pathogen, is among the most prevalent and serious threats to the world's shrimp culture industry. Its virulence typically manifests itself through shrimp hepatopancreas destruction. In the current study, we found that NO generated by an in vitro donor system (NOC-18) could rapidly and effectively kill V harveyi. In addition, injection of heat-killed V. harveyi increased the concentration of NO/nitrite and the mRNA expression of nitric oxide synthase (NOS) in the hepatopancreas of Pacific white shrimp (Litopenaeus vannamei), the commercially most significant shrimp species. Live V. harveyi challenge also induced NO/nitrite production and NOS gene expression in primary L. vannamei hepatopancreatic cells in a time- and dose-dependent manner. Co-incubation of L-NAME, an inhibitor selective for mammalian constitutive NOSs, dose-dependently blocked V harveyi-induced NO/nitrite production, without affecting V harveyi-induced NOS mRNA expression. Furthermore, L-NAME treatment significantly increased the survival rate of infecting V harveyi in cultured primary hepatopancreatic cells of L. vannamei. As a whole, we have demonstrated that endogenous NO produced by L. vannamei hepatopancreatic cells occurs in enzymatically regulated manners and is sufficient to act as a bactericidal molecule for V. harveyi clearance. (C) 2014 Elsevier Ltd. All rights reserved.
部门归属LMB
关键词Nitric Oxide Antimicrobial Molecule Vibrio Harveyi Litopenaeus Vannamei Hepatopanfcreas
学科领域Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14899
专题中科院海洋生物资源可持续利用重点实验室
通讯作者chan1010@scsio.ac.cn; wongnk@gmail.com; jiangxiao4367@126.com; luoxingzky@163.com; lpzhang@scsio.ac.cn; yangdan@hku.hk; rosemary166@sina.com; hucq@scsio.ac.cn
作者单位1.[Chen, Ting
2.Jiang, Xiao
3.Luo, Xing
4.Zhang, Lvping
5.Ren, Chunhua
6.Hu, Chaoqun] Chinese Acad Sci, CAS Key Lab Trop Marine Bioresources & Ecol LMB, Key Lab Appl Marine Biol Guangdong Prov, South China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China
7.[Wong, Nai-Kei
8.Yang, Dan] Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China
9.[Luo, Xing] Univ Chinese Acad Sci, Beijing, Peoples R China
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Chen, Ting,Wong, Nai-Kei,Jiang, Xiao,et al. Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2015,42(1):114-120.
APA Chen, Ting.,Wong, Nai-Kei.,Jiang, Xiao.,Luo, Xing.,Zhang, Lvping.,...&hucq@scsio.ac.cn.(2015).Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,42(1),114-120.
MLA Chen, Ting,et al."Nitric oxide as an antimicrobial molecule against Vibrio harveyi infection in the hepatopancreas of Pacific white shrimp, Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 42.1(2015):114-120.
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