Proteomic Basis of Stress Responses in the Gills of the Pacific Oyster Crassostrea gigas
Zhang, Yang1; Sun, Jin1; Mu, Huawei1; Li, Jun1; Zhang, Yuehuan1; Xu, Fengjiao1; Xiang, Zhiming1; Qian, Pei-Yuan1; Qiu, Jian-Wen1; Yu, Ziniu1; qiujw@hkbu.edu.hk; carlzyu@scsio.ac.cn
2015
发表期刊JOURNAL OF PROTEOME RESEARCH
卷号14期号:1页码:304-317
摘要The Pacific oyster Crassostrea gigas is one of the dominant sessile inhabitants of the estuarine intertidal zone, which is a physically harsh environment due to the presence of a number of stressors. Oysters have adapted to highly dynamic and stressful environments, but the molecular mechanisms underlying such stress adaptation are largely unknown. In the present study, we examined the proteomic responses in the gills of C. gigas exposed to three stressors (high temperature, low salinity, and aerial exposure) they often encounter in the field. We quantitatively compared the gill proteome profiles using iTRAQ-coupled 2-D LC-MS/MS. There were 3165 identified proteins among which 2379 proteins could be quantified. Heat shock, hyposalinity, and aerial exposure resulted in 50, 15, and 33 differentially expressed gill proteins, respectively. Venn diagram analysis revealed substantial different responses to the three stressors. Only xanthine dehydrogenase/oxidase showed a similar expression pattern across the three stress treatments, suggesting that reduction of ROS accumulation may be a conserved response to these stressors. Heat shock caused significant overexpression of molecular chaperones and production of S-adenosyl-l-methionine, indicating their crucial protective roles against protein denature. In addition, heat shock also activated immune responses, Ca2+ binding protein expression. By contrast, hyposalinity and aerial exposure resulted in the up-regulation of 3-demethylubiquinone-9 3-methyltransferase, indicating that increase in ubiquinone synthesis may contribute to withstanding both the osmotic and desiccation stress. Strikingly, the majority of desiccation-responsive proteins, including those involved in metabolism, ion transportation, immune responses, DNA duplication, and protein synthesis, were down-regulated, indicating conservation of energy as an important strategy to cope with desiccation stress. There was a high consistency between the expression levels determined by iTRAQ and Western blotting, highlighting the high reproducibility of our proteomic approach and its great value in revealing molecular mechanisms of stress responses.
部门归属LMB
关键词Oyster Gills Itraq Crassostrea Temperature Salinity Aerial Exposure
学科领域Biochemistry & Molecular Biology
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文献类型期刊论文
条目标识符http://ir.scsio.ac.cn/handle/344004/14900
专题中科院海洋生物资源可持续利用重点实验室
通讯作者qiujw@hkbu.edu.hk; carlzyu@scsio.ac.cn
作者单位1.[Zhang, Yang
2.Li, Jun
3.Zhang, Yuehuan
4.Xu, Fengjiao
5.Xiang, Zhiming
6.Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China
7.[Sun, Jin
8.Mu, Huawei
9.Qiu, Jian-Wen] Hong Kong Baptist Univ, Dept Biol, Kowloon Tong, Hong Kong, Peoples R China
10.[Qian, Pei-Yuan] Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Hong Kong, Peoples R China
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Zhang, Yang,Sun, Jin,Mu, Huawei,et al. Proteomic Basis of Stress Responses in the Gills of the Pacific Oyster Crassostrea gigas[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(1):304-317.
APA Zhang, Yang.,Sun, Jin.,Mu, Huawei.,Li, Jun.,Zhang, Yuehuan.,...&carlzyu@scsio.ac.cn.(2015).Proteomic Basis of Stress Responses in the Gills of the Pacific Oyster Crassostrea gigas.JOURNAL OF PROTEOME RESEARCH,14(1),304-317.
MLA Zhang, Yang,et al."Proteomic Basis of Stress Responses in the Gills of the Pacific Oyster Crassostrea gigas".JOURNAL OF PROTEOME RESEARCH 14.1(2015):304-317.
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