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cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei
Li, Guang-li; Deng, Si-ping; Jiang, Shu-na; Ye, Man; Chen, Hua-pu; Chan, Siuming F.; Zhu, Chun-hua; zhu860025@163.com
2015
Source PublicationGENES & GENOMICS
Volume37Issue:10Pages:883-892
AbstractIn this study, the full-length GIH cDNA sequence from Litopenaeus vannamei was cloned from the eyestalk by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends. The full-length GIH cDNA was 865 bp with a 288 bp open-reading frame, which encoded a 96 amino acid prepro-GIH with 17 amino acid signal peptide. L. vannamei GIH (LvGIH) can be classified as a member of type-II crustacean hyperglycemic hormone polypeptide family. LvGIH shares 93.8 and 66.7 % amino acid sequence identity with GIH from Penaeus monodon, and the molt-inhibiting hormone from Marsupenaeus japonicas, respectively. By quantitative real-time PCR (qPCR), LvGIH mRNA transcripts were detected in fertilized eggs, nauplius, zoea, mysis and juveniles of 25, 35 and 40 days old. LvGIH transcript levels increased significantly with the development from fertilized eggs to juveniles. LvGIH transcript levels were highest in juveniles at 35 days old. By RT-PCR, LvGIH mRNA transcripts were detected only in the eyestalks and brains but not in the muscles, intestines, gills, heart, hepatopancreas, ovaries and testes of adults, and there was no difference in the expression level of LvGIH between males and females. Using the P. monodon anti-GIH antibody, we showed that LvGIH was located mainly in the XO-SG and slightly in axon, with similar fluorescence intensity found in XO and SG. To summarize, we have cloned and characterized the GIH of the shrimp L. vannamei. In addition to the GIH properties described in other crustaceans, a peak of LvGIH expression was identified at the time of sexual differentiation (i.e., day 35 larvae) suggesting that LvGIH may also be involved in the control of this process.
Department[Li, Guang-li; Deng, Si-ping; Jiang, Shu-na; Ye, Man; Chen, Hua-pu; Chan, Siuming F.; Zhu, Chun-hua] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China; [Li, Guang-li; Deng, Si-ping; Jiang, Shu-na; Ye, Man; Chen, Hua-pu; Chan, Siuming F.; Zhu, Chun-hua] Guangdong Ocean Univ, Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ An, Zhanjiang 524088, Peoples R China ; LTO
KeywordLitopenaeus Vannamei Gonad-inhibiting Hormone Cloning Expression
Subject AreaBiochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
Document Type期刊论文
Identifierhttp://ir.scsio.ac.cn/handle/344004/14912
Collection热带海洋环境国家重点实验室(LTO)
Corresponding Authorzhu860025@163.com
Recommended Citation
GB/T 7714
Li, Guang-li,Deng, Si-ping,Jiang, Shu-na,et al. cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei[J]. GENES & GENOMICS,2015,37(10):883-892.
APA Li, Guang-li.,Deng, Si-ping.,Jiang, Shu-na.,Ye, Man.,Chen, Hua-pu.,...&zhu860025@163.com.(2015).cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei.GENES & GENOMICS,37(10),883-892.
MLA Li, Guang-li,et al."cDNA cloning, expression, and immunolocalization of gonad-inhibiting hormone (GIH) in Litopenaeus vannamei".GENES & GENOMICS 37.10(2015):883-892.
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